Lysinibacillus boronitolerans

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Lysinibacillus boronitolerans is a Gram-positive, spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 36.5%. Lysinibacillus boronitolerans is probably a rare gut coloniser. (Ahmed2007; Lee2010)



  • This organism has been recovered from contaminated soil from Japan. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • Tolerates 100 mM boron, with some strains able to grow with 150 mM boron.
    • GENERAL CHARACTERISTICS (Ahmed2007); (Lee2010);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; DNA; gelatin; urea;
  • 🧂
  • Salt tolerance:
  • tolerates 5% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 5.5; tolerates pH 9.5; Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 16℃; grows at 45℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • xylose; N-Ac glucosamine;
  • ⥅
  • Substrates assimilated or utilised:
  • cellubiose; erythritol; aesculin; fructose; gluconate; glucose; inositol; ribose; D-tagatose; alanine; D-alanine; aspartate; glutamate; histidine; leucine; ornithine; proline; serine; threonine; acetate; aconitate; citrate; formate; 2-ketogluconate; 5-ketogluconate; DL-lactate; L-malate; propionate; pyruvate; fructose-6-phosphate; glutamine; L-asparagine; alaninamide; α-ketovalerate; α-ketobutyrate; 2-hydroxybutyrate; methyl succinate; methyl pyruvate; lactamide; thymidine; uridine; inosine; L-lactate; AMP; TMP; UMP; N-acetylmannosamine;
  • Active enzymes:
  • alkaline phosphatase; arginine dihydrolase; catalase; esterase C4; esterase lipase C8; lipase C14; oxidase; pyroGlu arylamidase; urease;
    • SPECIAL FEATURES (Ahmed2007); (Lee2010);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Ahmed2007); (Lee2010);
    Class Active Resistant
  • Penicillins:
  • carbenicillin; penicillin G;
  • ampicillin;
  • Cephalosporins:
  • cephalothin;
  • Macrolides:
  • erythromycin; oleandomycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • kanamycin; neomycin; streptomycin;
  • gentamicin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • chloramphenicol;
  • fusidic-acid; metronidazole;
  • Miscellaneous antibiotics:
  • lincomycin; colistin; novobiocin; polymyxin B;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Caryophanales Family:  Caryophanaceae Genus:  Lysinibacillus Gram stain:  + O2 Relation.:  aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  contaminated soil from Japan
    DNA G+C(%):  36.5
    Opt. T:  37℃
    Low T(℃):  16(+)
    High T(℃):  45(+)
    NaCl 3-5%:  5(+)
    Opt. pH:  7.0
    pH 4.2-5.9:  5.5(+)
    pH >8:  9.5(+)
    		Aesculin:  + Urea:  + Gelatin:  + Starch:  neg Casein:  neg DNA:  + Tyrosine:  neg Tween:  20(+) 40(+) 60(+) 80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg D-Lyxose:  neg Rhamnose:  neg Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Aesculin:  w Glycogen:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  + Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + D-Lyxose:  neg Mannose:  neg Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  + Xylose:  neg Cellubiose:  + Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg N_Acetyl_glucosamine:  neg Adonitol:  neg Amygdalin:  neg D-Arabitol:  neg L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  + Aesculin:  + Gluconate:  + Glycerol:  neg Glycogen:  neg Inositol:  + Inulin:  neg Mannitol:  neg Me-α-D-Glc:  neg Salicin:  neg Sorbitol:  neg Starch:  neg Xylitol:  neg Ala:  + D-Ala:  + Asp:  + Glu:  + His:  + Leu:  + Orn:  + Pro:  + Ser:  + Thr:  + Acetate:  + Aconitate:  cis(+) Citrate:  + Formate:  + 3-Hydroxybutyrate:  neg 2-Ketogluconate:  + 5-Ketogluconate:  + DL-Lactate:  + L-Malate:  + Propionate:  + Pyruvate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  + Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  + Chymotrypsin:  w LysDC:  neg OrnDC:  neg TrpAA:  neg LeuAA:  w PyrogluAA:  + ValAA:  w AlkalineP:  + AcidP:  w Esterase(C4):  + EstLip(C8):  + Lecithinase:  neg Lipase(C14):  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  Res
    carbenicil:  Sens
    penicillin_G:  Sens
    cephalothin:  Res
    gentamicin:  Res
    kanamycin:  Sens
    neomycin:  Sens
    streptomycin:  S(8)
    erythromycin:  S(1)
    oleandomycin:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(2)
    rifampicin:  S(0.25)
    chloramphenicol:  S(8)
    metronidazole:  Res
    lincomycin:  Res
    novobiocin:  Res
    colistin:  Res
    polymyxin_B:  Res
    fusidic-acid:  Res

    References

    SPECIFIC REFERENCES FOR LYSINIBACILLUS BORONITOLERANS
  • Ahmed2007 - Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.
  • Lee2010 - Lysinibacillus xylanilyticus sp. nov., a xylan-degrading bacterium isolated from forest humus.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR LYSINIBACILLUS BORONITOLERANS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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    • Added: January 14, 2022