Massilia aurea

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Massilia aurea is a Gram-negative, non-spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 66.0%. Massilia aurea is likely to be transient and not a long-term gut coloniser. (Gallego2006; Editorial2005Bergey)



  • This organism has been recovered from human faeces, and drinking water. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Borderline cold mesophile, with optimum growth temp of 28C. Unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Gallego2006); (Editorial2005Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; casein; DNA; starch;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 2% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 4.2; tolerates pH 6.0-8.0; tolerates pH 9; Grows optimally at pH 6.0-7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 4℃; grows at 30℃; Grows optimally at 28℃.
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; amygdalin; cellubiose; aesculin; fructose; gentiobiose; glucose; glycogen; maltose; mannose; starch; xylose; adipate; citrate; malate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; esterase C4; esterase lipase C8; β-galactosidase; α-glucosidase; Leu arylamidase; Val arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Gallego2006);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • yellow
    • RESPONSE TO ANTIBIOTICS (Gallego2006);
    Class Active Resistant
  • Penicillins:
  • penicillin;
  • Cephalosporins:
  • cephalothin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • kanamycin; neomycin; streptomycin;
  • Polypep/ketides:
  • rifampicin;
  • bacitracin;
  • Heterocycles:
  • chloramphenicol;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • novobiocin;

  • McLaughlin, S. D., Walker, A. W., Churcher, C., Clark, S. K., Tekkis, P. P., Johnson, M. W., Parkhill, J., Ciclitira, P. J., Dougan, G., Nicholls, R. J., & Petrovska, L. (2010). The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing. Annals of Surgery, 252(1), 90–98.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Oxalobacteraceae Genus:  Massilia Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  yellow
    Health:  Unknown
    Source:  human faeces, and drinking water
    DNA G+C(%):  66.0
    Opt. T:  28℃
    Low T(℃):  4(+)
    Lower T(℃):  30(+)
    NaCl 0.5-2%:  2(neg)
    Opt. pH:  6.0-7.0
    pH 4.2-5.9:  4.2(+)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  9(+)
    		Aesculin:  + Urea:  neg Gelatin:  vr Starch:  + Casein:  + DNA:  + Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + D-Lyxose:  neg Mannose:  + Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  + Cellubiose:  + Gentiobiose:  + Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  neg D-Turanose:  neg Trehalose:  neg N_Acetyl_glucosamine:  + Adonitol:  neg Amygdalin:  + D-Arabitol:  neg L-Arabitol:  neg Arbutin:  neg Dulcitol:  neg Erythritol:  neg Aesculin:  + Gluconate:  neg Glycerol:  neg Glycogen:  + Inositol:  neg Inulin:  neg Mannitol:  neg Me-α-D-Glc:  neg Salicin:  neg Sorbitol:  neg Starch:  + Xylitol:  neg Adipate:  + Caprate:  neg Citrate:  + 2-Ketogluconate:  neg 5-Ketogluconate:  neg Malate:  + Phenylacetate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  w Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  neg LysDC:  neg OrnDC:  neg Trypsin:  neg TrpAA:  neg CystineAA:  neg LeuAA:  + ValAA:  + AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  + Lipase:  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  yellow
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    penicillin:  R(10U)
    cephalothin:  R(30; disc)
    kanamycin:  S(30; disc)
    neomycin:  S(10; disc)
    streptomycin:  S(10; disc)
    erythromycin:  S(15; disc)
    nalidixic-acid:  S(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(30; disc)
    vancomycin:  S(30; disc)
    bacitracin:  R(10U)
    rifampicin:  S(30; disc)
    chloramphenicol:  S(30; disc)
    novobiocin:  S(30; disc)

    References

    SPECIFIC REFERENCES FOR MASSILIA AUREA
  • Gallego2006 - Massilia aurea sp. nov., isolated from drinking water.
  • Editorial2005Bergey - Bergey's manual of systematic bacteriology. Vol. 2, The Alpha-, Beta-, Delta-, and Epsilonproteobacteria Part C. Family Oxalobacteraceae, Genus V. Massilia
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MASSILIA AUREA
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
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    • Added: April 08, 2021