Massilia timonae

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Massilia timonae is a Gram-negative, non-spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 64.6%. Massilia timonae is unlikely to be a gut coloniser. (Kampfer2011; Lindquist2003; LaScola1998)



  • This organism has been recovered from clinical sources (blood). The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). It is an opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Significant variation reported for assimilation of some substrates between references. Kampfer2011 used to override discrepancies. Likely a gut non-coloniser.
    • GENERAL CHARACTERISTICS (Kampfer2011); (Lindquist2003); (LaScola1998);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; gelatin; pectin; starch;
  • 🧂
  • Salt tolerance:
  • tolerates 0% salt; doesn't tolerate 3% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 35℃; doesn't grow at 42℃;
  • H+
  • Acid from carbohydrates usually produced:
  • fructose;
  • ⥅
  • Substrates assimilated or utilised:
  • L-arabinose; cellubiose; gluconate; glucose; glycogen; lactose; maltose; mannose; rhamnose; D-tagatose; xylose; alanine; histidine; proline; serine; citrate; fumarate; 3-hydroxybutyrate; 2-ketoglutarate; lactate; L-malate; malonate; succinate; D-glucuronate; D-malate; gentisate;
  • Active enzymes:
  • arginine dihydrolase; catalase; oxidase;
    • SPECIAL FEATURES (Lindquist2003);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S;
  • ±
  • Nitrate:
  • reduced by few strains
    • RESPONSE TO ANTIBIOTICS (LaScola1998);
    Class Active Resistant
  • Penicillins:
  • imipenem; ticarcillin;
  • Cephalosporins:
  • cefotaxime; ceftazidime;
  • cephalothin;
  • Quinolines:
  • ciprofloxacin; ofloxacin; pefloxacin;
  • Aminoglycosides:
  • amikacin; gentamicin; netilmycin; tobramycin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;
  • fosfomycin;
  • Miscellaneous antibiotics:
  • colistin; polymyxin B;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Oxalobacteraceae Genus:  Massilia Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  clinical sources (blood)
    DNA G+C(%):  64.6
    Lower T(℃):  25(+)
    Mid T(℃):  35(+)
    High T(℃):  42(neg)
    NaCl 0.5-2%:  0(+)
    NaCl 3-5%:  3(neg)
    		Aesculin:  + Urea:  neg Gelatin:  + Starch:  + Pectin:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Glucose:  w Mannose:  neg Rhamnose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Sucrose:  neg Glycogen:  neg Mannitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  + Fructose:  neg Galactose:  neg Glucose:  + Mannose:  + Rhamnose:  + D-Tagatose:  + Xylose:  + Cellubiose:  + Lactose:  + Maltose:  + Melezitose:  neg Sucrose:  neg Trehalose:  neg Gluconate:  + Glycogen:  + Ala:  + D-Ala:  neg Asp:  neg Glu:  neg His:  + Pro:  + Ser:  + Adipate:  neg Citrate:  + Fumarate:  + DL-Glycerate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Lactate:  + DL-Lactate:  neg L-Malate:  + Malonate:  + Mucate:  neg Propionate:  neg Saccharate:  neg Suberate:  neg Succinate:  + L-Tartrate:  neg Valerate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg ArgDH:  + LysDC:  neg OrnDC:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  Var(MIC50): 4), MIC90: Var(4
    Augmentin:  Var(MIC50): 4), MIC90: Var(4
    penicillin_G:  Var(MIC50): 8), MIC90: Var(8
    piperacillin:  Var(MIC50): 4), MIC90: Var(4
    ticarcillin:  S(2)
    imipenem:  S(0.5)
    cefotaxime:  S(0.125)
    ceftazidime:  S(1)
    cephalothin:  R(16)
    amikacin:  S(1)
    gentamicin:  S(0.5)
    netilmycin:  S(0.25)
    tobramycin:  S(2)
    ciprofloxacin:  S(<0.125)
    ofloxacin:  S(<0.125)
    pefloxacin:  S(<0.125)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    rifampicin:  S(0.25)
    fosfomycin:  R(32)
    SXT:  S(<0.03)
    colistin:  S(<2)
    polymyxin_B:  Sens

    References

    SPECIFIC REFERENCES FOR MASSILIA TIMONAE
  • Kampfer2011 - Revision of the genus Massilia La Scola et al. 2000, with an emended description of the genus and inclusion of all species of the genus Naxibacter as new combinations, and proposal of Massilia consociata sp. nov.
  • Lindquist2003 - Characteristics of Massilia timonae and Massilia timonae-like isolates from human patients, with an emended description of the species.
  • LaScola1998 - Massilia timonae gen. nov., sp. nov., isolated from blood of an immunocompromised patient with cerebellar lesions.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MASSILIA TIMONAE
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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    • Added: January 14, 2022