Megasphaera indica

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Megasphaera indica is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, coccus bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 54.9-57.7%. Megasphaera indica is probably a rare gut coloniser. (Lanjekar2014)



  • This organism has been recovered from human faeces (Lanjekar2014). Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal. Robust growth can have positive consequences for gut health.
    • GENERAL CHARACTERISTICS (Lanjekar2014);
    Character Response
  • 🌡
  • Temperature tolerance:
  • grows at 15℃; grows at 37℃; doesn't grow at 45℃; Grows optimally at 37℃.
  • ⥅
  • Substrates assimilated or utilised:
  • amygdalin; arbutin; cellubiose; dextrin; fructose; galactose; gluconate; glucose; lactose; maltose; mannitol; mannose; rhamnose; trehalose; methionine; phenylalanine; DL-lactate; pyruvate; lactulose; L-asparagine; uridine; inosine;
  • ±
  • Strain-dependent substrate utilisation:
  • adonitol; maltotriose; alaninamide; α-ketovalerate;
    • SPECIAL FEATURES (Lanjekar2014);
    Character Response
  • ✓
  • Metabolites produced:
  • formate (minor); acetate; butyrate; valerate; isobutyrate; succinate (minor); isovalerate; H₂S; CO₂; H₂;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Lanjekar2014);
    Class Active Resistant
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • colistin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Negativicutes Order:  Veillonellales Family:  Veillonellaceae Genus:  Megasphaera Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus
    Health:   Positive
    Source:  human faeces (Lanjekar2014)
    DNA G+C(%):  54.9-57.7
    Opt. T:  37℃
    Low T(℃):  15(+)
    Mid T(℃):  37(+)
    High T(℃):  45(neg)
    		Aesculin:  neg Gelatin:  neg Arginine:  neg Milk:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  + Cellubiose:  + Lactose:  + Maltose:  + Maltotriose:  d Trehalose:  + Adonitol:  d Amygdalin:  + Arbutin:  + Dextrin:  + Gluconate:  + Mannitol:  + Met:  + Phe:  + Thr:  neg 3-Hydroxybutyrate:  neg DL-Lactate:  + L-Malate:  neg Pyruvate:  + Succinate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  minor(+) Acetate:  + Butyrate:  + Valerate:  + Isobutyrate:  + Succinate:  minor(+) Isovalerate:  + H2S:  + CO2:  + H2:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    vancomycin:  R(5; disc)
    colistin:  R(10; disc)

    References

    SPECIFIC REFERENCES FOR MEGASPHAERA INDICA
  • Lanjekar2014 - Megasphaera indica sp. nov., an obligate anaerobic bacteria isolated from human faeces.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MEGASPHAERA INDICA
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • Zou2019 - 1,520 reference genomes from cultivated human gut bacteria enable functional microbiome analyses.
  • ...............................
    • GENERAL REFERENCES FOR MEGASPHAERA INDICA
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: July 12, 2021