Megasphaera massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Megasphaera massiliensis is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, coccobacillus bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 50.2%. Megasphaera massiliensis is probably a rare gut coloniser. (Padmanabhan2013)



  • This organism has been recovered from human faeces (HIV patient - Padmanabhan2013). Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Padmanabhan2013);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin;
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 45℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; fructose; fucose; galactose; glucose; mannose; rhamnose; ribose; xylose; aesculin; cellubiose; gentiobiose; lactose; maltose; trehalose; D-arabitol; mannitol; sorbitol; arbutin; gluconate; 5-ketogluconate; N-Ac glucosamine; salicin;
  • Active enzymes:
  • α-glucosidase; β-glucosidase; oxidase;
    • SPECIAL FEATURES (Padmanabhan2013);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
    • RESPONSE TO ANTIBIOTICS (Padmanabhan2013);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; amoxicillin-clavulanic acid; imipenem; ticarcillin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline;
  • Quinolines:
  • ciprofloxacin;
  • Aminoglycosides:
  • amikacin; gentamicin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • trimethoprim-sulfamethoxazole;
  • fosfomycin; metronidazole; nitrofurantoin;
  • Vancomycins:
  • vancomycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Negativicutes Order:  Veillonellales Family:  Veillonellaceae Genus:  Megasphaera Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccobacillus
    Health:  Unknown
    Source:  human faeces (HIV patient - Padmanabhan2013)
    DNA G+C(%):  50.2
    Opt. T:  37℃
    Lower T(℃):  30(+)
    High T(℃):  45(+)
    		Gelatin:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  w L-Arabinose:  + Fructose:  + Fucose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  + Ribose:  + Xylose:  + Cellubiose:  + Gentiobiose:  + Lactose:  + Maltose:  + Sucrose:  neg Trehalose:  + Amygdalin:  w Aesculin:  + D-Arabitol:  + Glycerol:  w Mannitol:  + Sorbitol:  + Arbutin:  + Gluconate:  + 2-Ketogluconate:  w 5-Ketogluconate:  + NAc-α-GA:  + Salicin:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  neg Urease:  neg α-Arab:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg AlanineAA:  neg ArgAA:  neg GluGluAA:  neg GlyAA:  neg HisAA:  neg LeuAA:  neg LeuGlyAA:  neg ProAA:  neg PheAA:  neg PyrogluAA:  neg SerAA:  neg TyrAA:  neg AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  Sens
    Augmentin:  Sens
    ticarcillin:  Sens
    imipenem:  Sens
    amikacin:  Sens
    gentamicin:  Sens
    erythromycin:  Res
    ciprofloxacin:  Res
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  Sens
    vancomycin:  Res
    rifampicin:  Res
    fosfomycin:  Res
    metronidazole:  Res
    nitrofurantoin:  Res
    SXT:  Sens

    References

    SPECIFIC REFERENCES FOR MEGASPHAERA MASSILIENSIS
  • Padmanabhan2013 - Non-contiguous finished genome sequence and description of Megasphaera massiliensis sp. nov.
  • Dong2020 - A Microbial Signature Identifies Advanced Fibrosis in Patients with Chronic Liver Disease Mainly Due to NAFLD
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MEGASPHAERA MASSILIENSIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • Urban2020 - Altered Fecal Microbiome Years after Traumatic Brain Injury
  • ...............................
    • GENERAL REFERENCES FOR MEGASPHAERA MASSILIENSIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: July 12, 2021