Methylobacterium jeotgali

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Methylobacterium jeotgali is a Gram-negative, non-spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 64.9%. Methylobacterium jeotgali is unlikely to be a gut coloniser. (Aslam2007)



  • This organism has been recovered from human faeces, and fermented seafood. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe, no spore formation and doesn't grow at 37C, so probably a gut non-coloniser.
    • GENERAL CHARACTERISTICS (Aslam2007);
    Character Response
  • Substrates hydrolysed or digested:
  • casein; urea;
  • 🧂
  • Salt tolerance:
  • tolerates 1% salt; doesn't tolerate 3% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0-8.0; tolerates pH 10; Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 20-30℃; doesn't grow at 37℃; Grows optimally at 30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; L-xylose; D-lyxose; glycerol;
  • ⥅
  • Substrates assimilated or utilised:
  • ethanol; methanol; rhamnose; xylose; betaine; proline; serine; acetate; 3-hydroxybutyrate; 3-hydroxybenzoate; 4-hydroxybenzoate; DL-lactate; malonate;
  • Active enzymes:
  • arginine dihydrolase; catalase; oxidase; urease;
    • SPECIAL FEATURES (Aslam2007);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S; indole;
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS (Aslam2007);
    Class Active Resistant
  • Penicillins:
  • ampicillin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • kanamycin;
  • Polypep/ketides:
  • rifampicin;

  • McLaughlin, S. D., Walker, A. W., Churcher, C., Clark, S. K., Tekkis, P. P., Johnson, M. W., Parkhill, J., Ciclitira, P. J., Dougan, G., Nicholls, R. J., & Petrovska, L. (2010). The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing. Annals of Surgery, 252(1), 90–98.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Methylobacteriaceae Genus:  Methylobacterium Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  neg
    Health:  Unknown
    Source:  human faeces, and fermented seafood
    DNA G+C(%):  64.9
    Opt. T:  30℃
    Lower T(℃):  20-30(+)
    Mid T(℃):  37(neg)
    NaCl 0.5-2%:  1(+)
    NaCl 3-5%:  3(neg)
    Opt. pH:  7.0
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  10(+)
    		Aesculin:  w Urea:  + Gelatin:  w Starch:  neg Casein:  + Chitin:  neg DNA:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  + L-Arabinose:  neg Fructose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg D-Lyxose:  + Rhamnose:  neg Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  + Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  + Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  neg Fucose:  neg Glucose:  neg Rhamnose:  + Ribose:  neg Xylose:  + Maltose:  neg Melibiose:  neg Sucrose:  neg N_Acetyl_glucosamine:  neg Ethanol:  + Glycogen:  neg Inositol:  neg Mannitol:  neg Methanol:  + Salicin:  neg Sorbitol:  neg Ala:  neg Betaine:  + His:  neg Pro:  + Ser:  + Acetate:  + Caprate:  neg Citrate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  + 4-Hydroxybenzoate:  + Itaconate:  neg 5-Ketogluconate:  neg DL-Lactate:  + Malonate:  + Propionate:  neg Suberate:  neg Valerate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  + β-Glucosidase:  neg ArgDH:  + LysDC:  neg OrnDC:  neg DNAse:  neg Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  R(100; disc)
    kanamycin:  S(50; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  R(100; disc)
    rifampicin:  R(100; disc)

    References

    SPECIFIC REFERENCES FOR METHYLOBACTERIUM JEOTGALI
  • Aslam2007 - Methylobacterium jeotgali sp. nov., a non-pigmented, facultatively methylotrophic bacterium isolated from jeotgal, a traditional Korean fermented seafood.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR METHYLOBACTERIUM JEOTGALI
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • Added: April 08, 2021