Methylobacterium oryzae

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Methylobacterium oryzae is a Gram-negative, non-spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 70.6%. Methylobacterium oryzae is likely to be transient and not a long-term gut coloniser. (Madhaiyan2007)



  • This organism has been recovered from rice and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Borderline cold mesophile, with optimum growth temp of 28C. Unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Madhaiyan2007);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 2% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 5.0; tolerates pH 10; Grows optimally at pH 6.8.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 4℃; grows at 20-30℃; doesn't grow at 40℃; Grows optimally at 28℃.
  • ⥅
  • Substrates assimilated or utilised:
  • arabinose; L-arabinose; ethanol; galactose; gluconate; glycerol; methanol; xylose; aspartate; glutamate; formate; fumarate; 3-hydroxybutyrate; 2-ketoglutarate; DL-lactate; malate; malonate; propionate; saccharate; succinate; DL-glycerol phosphate; glutamine; L-asparagine; alaninamide; pyroglutamate; α-ketovalerate; α-ketobutyrate; 2-hydroxybutyrate; 4-hydroxybutyrate; succinamate; methyl pyruvate; urea;
  • ±
  • Strain-dependent substrate utilisation:
  • fructose; acetate; adipate; citrate; sebacate;
  • Active enzymes:
  • catalase; esterase C4; Leu arylamidase; oxidase;
    • SPECIAL FEATURES (Madhaiyan2007);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✓
  • NO3➔NO2:
  • reduced
  • Pigments:
  • pink
    • RESPONSE TO ANTIBIOTICS (Madhaiyan2007);
    Class Active Resistant
  • Penicillins:
  • ampicillin; carbenicillin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; kanamycin; spectinomycin;
  • streptomycin;
  • Heterocycles:
  • chloramphenicol;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Methylobacteriaceae Genus:  Methylobacterium Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  pink
    Health:  Unknown
    Source:  rice and human faeces
    DNA G+C(%):  70.6
    Opt. T:  28℃
    Low T(℃):  4(neg)
    Lower T(℃):  20-30(+)
    Mid T(℃):  40(neg)
    NaCl 0.5-2%:  2(+)
    Opt. pH:  6.8
    pH 4.2-5.9:  5.0(+)
    pH >8:  10(+)
    		Aesculin:  neg Gelatin:  neg Starch:  neg Casein:  neg Pectin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  + Fructose:  d Fucose:  neg Galactose:  + Glucose:  neg Xylose:  + Sucrose:  neg Ethanol:  + Gluconate:  + Glycerol:  + Methanol:  + Asp:  + Betaine:  neg Glu:  + Lys:  neg Acetate:  d Adipate:  d Citrate:  d Formate:  + Fumarate:  + 3-Hydroxybutyrate:  + DL-Lactate:  + Malate:  + Malonate:  + Propionate:  + Saccharate:  + Sebacate:  d Succinate:  + D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Trypsin:  w LeuAA:  + ValAA:  w AcidP:  w Esterase(C4):  + EstLip(C8):  w Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  pink
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  Res
    carbenicil:  Res
    gentamicin:  Sens
    kanamycin:  Sens
    spectinomycin:  Sens
    streptomycin:  Res
    nalidixic-acid:  Res
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  Sens
    chloramphenicol:  Res

    References

    SPECIFIC REFERENCES FOR METHYLOBACTERIUM ORYZAE
  • Madhaiyan2007 - Methylobacterium oryzae sp. nov., an aerobic, pink-pigmented, facultatively methylotrophic, 1-aminocyclopropane-1-carboxylate deaminase-producing bacterium isolated from rice.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR METHYLOBACTERIUM ORYZAE
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • ...............................
    • Added: December 15, 2021