Methylorubrum populi

(aka Methylobacterium populi)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Methylorubrum populi, (aka Methylobacterium populi), is a Gram-negative, non-spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 70.4%. Methylorubrum populi is likely to be transient and not a long-term gut coloniser. (Green2018; Aken2004)



  • This organism has been recovered from human faeces, and poplar tree rhizosphere. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe; unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Green2018); (Aken2004);
    Character Response
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 2% salt;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 4; doesn't tolerate pH 9; Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 15℃; doesn't grow at 40℃; Grows optimally at 20-30℃.
  • ⥅
  • Substrates assimilated or utilised:
  • ethanol; fructose; glycerol; methanol; betaine; acetate; formate; fumarate; lactate; pyruvate; succinate; D-tartrate; L-tartrate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; chymotrypsin; esterase C4; esterase lipase C8; oxidase; urease; Val arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Green2018); (Aken2004);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ±
  • Pigments:
  • pinkish-red

  • Turnbaugh, P. J., Hamady, M., Yatsunenko, T., Cantarel, B. L., Duncan, A., Ley, R. E., Sogin, M. L., Jones, W. J., Roe, B. A., Affourtit, J. P., Egholm, M., Henrissat, B., Heath, A. C., Knight, R., & Gordon, J. I. (2009). A core gut microbiome in obese and lean twins. Nature, 457(7228), 480–484.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Methylobacteriaceae Genus:  Methylorubrum Alt. name:  Methylobacterium populi Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  pinkish-red
    Health:  Unknown
    Source:  human faeces, and poplar tree rhizosphere
    DNA G+C(%):  70.4
    Opt. T:  20-30℃
    Low T(℃):  15(neg)
    Mid T(℃):  40(neg)
    NaCl 0.5-2%:  2(neg)
    Opt. pH:  7.0
    pH 4.2-5.9:  4(neg)
    pH >8:  9(neg)
    		Gelatin:  neg Starch:  neg Casein:  neg DNA:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  + D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg Xylose:  neg Lactose:  neg Sucrose:  neg Ethanol:  + Glycerol:  + Inositol:  neg Mannitol:  neg Methanol:  + Sorbitol:  neg Asp:  neg Betaine:  + Glu:  neg Gly:  neg Acetate:  + Citrate:  neg Formate:  + Fumarate:  + Lactate:  + Pyruvate:  + Sebacate:  neg Succinate:  + D-Tartrate:  + L-Tartrate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  + β-Galactosidase:  neg Chymotrypsin:  + ValAA:  + AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  + Lecithinase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Pigment:  pinkish-red

    References

    SPECIFIC REFERENCES FOR METHYLORUBRUM POPULI
  • Green2018 - Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov.
  • Aken2004 - Methylobacterium populi sp. nov., a novel aerobic, pink-pigmented, facultatively methylotrophic, methane-utilizing bacterium isolated from poplar trees (Populus deltoides\texttimesnigra DN34).
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR METHYLORUBRUM POPULI
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021