Methylorubrum thiocyanatum

(aka Methylobacterium thiocyanatum)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Methylorubrum thiocyanatum, (aka Methylobacterium thiocyanatum), is a Gram-negative, non-spore-forming, strictly aerobic, non-motile, short rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 68.5%. Methylorubrum thiocyanatum is likely to be transient and not a long-term gut coloniser. (Green2018; Kato2005; Wood1998)



  • This organism has been recovered from soil from around tree roots. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Can utilise potassium thiocyanate as a nitrogen source and this compound as well as thiosulfate or tetrathionate as chemolithotrophic energy sources. Obligate aerobe so unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Green2018); (Kato2005); (Wood1998);
    Character Response
  • Substrates hydrolysed or digested:
  • urea;
  • pH
  • Acidity tolerance:
  • tolerates pH 7.5; tolerates pH 9.4;
  • 🌡
  • Temperature tolerance:
  • grows at 15℃; grows at 37℃; doesn't grow at 44℃; Grows optimally at 30-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; L-arabinose; fucose; ribose; xylose; L-xylose; D-lyxose; glycerol;
  • ⥅
  • Substrates assimilated or utilised:
  • fructose; glucose; methanol; betaine; glutamate; acetate; formate; fumarate; L-malate; pyruvate; succinate; glutamine; D-malate;
  • Active enzymes:
  • catalase; urease;
  • ±
  • Strain-dependent active enzymes:
  • oxidase;
    • SPECIAL FEATURES (Green2018); (Kato2005); (Wood1998);
    Character Response
  • ✓
  • Metabolites produced:
  • indole;
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S;
  • ✗
  • Methyl red test:
  • not active
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrite:
  • not reduced
  • ✗
  • NO3➔NO2:
  • not reduced
  • Pigments:
  • pink

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Alphaproteobacteria Order:  Hyphomicrobiales Family:  Methylobacteriaceae Genus:  Methylorubrum Alt. name:  Methylobacterium thiocyanatum Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Sessile Morphology:  Short Rod Pigment:  pink
    Health:  Unknown
    Source:  soil from around tree roots
    DNA G+C(%):  68.5
    Opt. T:  30-37℃
    Low T(℃):  15(+)
    Mid T(℃):  37(+)
    High T(℃):  44(neg)
    pH 6.0-8.0:  7.5(+)
    pH >8:  9.4(+)
    		Urea:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  + L-Arabinose:  + Fructose:  neg Fucose:  + D-Fucose:  w Galactose:  w Glucose:  w Mannose:  neg D-Lyxose:  + Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  w Xylose:  + L-Xylose:  + Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Aesculin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  + Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  w Fructose:  + Glucose:  + Mannose:  neg Maltose:  neg Sucrose:  neg N_Acetyl_glucosamine:  neg Gluconate:  neg Mannitol:  neg Methanol:  + Betaine:  + Glu:  + Acetate:  + Adipate:  neg Caprate:  neg Citrate:  neg Formate:  + Fumarate:  + L-Malate:  + Phenylacetate:  neg Pyruvate:  + Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  d Catalase:  + Urease:  + β-Galactosidase:  neg β-Glucosidase:  neg ArgDH:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  + Pigment:  pink

    References

    SPECIFIC REFERENCES FOR METHYLORUBRUM THIOCYANATUM
  • Green2018 - Review of the genus Methylobacterium and closely related organisms: a proposal that some Methylobacterium species be reclassified into a new genus, Methylorubrum gen. nov.
  • Kato2005 - Reclassification of Methylobacterium chloromethanicum and Methylobacterium dichloromethanicum as later subjective synonyms of Methylobacterium extorquens and of Methylobacterium lusitanum as a later subjective synonym of Methylobacterium rhodesianum.
  • Wood1998 - A novel pink-pigmented facultative methylotroph, Methylobacterium thiocyanatum sp. nov., capable of growth on thiocyanate or cyanate as sole nitrogen sources.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR METHYLORUBRUM THIOCYANATUM
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
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    • Added: February 09, 2022