Methyloversatilis universalis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Methyloversatilis universalis is a Gram-negative, non-spore-forming, aerobic, non-motile, rod-shaped bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 64-65%. Methyloversatilis universalis is probably a rare gut coloniser. (Kalyuzhnaya2006)



  • This organism has been recovered from human faeces, and contaminated soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Kalyuzhnaya2006);
    Character Response
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0-8.0; tolerates pH 8.5-9.0; Grows optimally at pH 7.5-8.
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 37℃; Grows optimally at 30-37℃.
  • ⥅
  • Substrates assimilated or utilised:
  • ethanol; fructose; glucose; glycerol; maltose; methanol; arginine; betaine; glycine; methionine; serine; citrate; formate; glycolate; malate; succinate; L-asparagine; methyl succinate; urea;
  • ±
  • Strain-dependent substrate utilisation:
  • mannitol; alanine; aspartate; cysteine; glutamate; histidine; phenylalanine; proline; sarcosine; threonine; tryptophan; acetate; oxalate; pyruvate; glutamine;
  • Active enzymes:
  • catalase; esterase C4; esterase lipase C8; Leu arylamidase; oxidase;
    • SPECIAL FEATURES (Kalyuzhnaya2006);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Kalyuzhnaya2006);
    Class Active Resistant
  • Penicillins:
  • amoxicillin;
  • ampicillin; penicillin;
  • Cephalosporins:
  • cephalothin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; kanamycin; neomycin; streptomycin;
  • Heterocycles:
  • chloramphenicol; trimethoprim-sulfamethoxazole;
  • Vancomycins:
  • vancomycin;
  • NOTES

    Able to utilise dimethylamine, formaldehyde, methanol and methylamine as 1-carbon substrates.

    The taxonomic positions and phylogenetic relationships of two new methylotrophic isolates from Lake Washington (USA) sediment, FAM5T and 500, and the previously described methylotrophic strain EHg5 isolated from contaminated soil in Estarreja (Portugal) were investigated. All three strains were facultative methylotrophs capable of growth on a variety of C1 and multicarbon compounds. Optimal growth occurred at pH 7.5-8 and 30-37 degrees C. The major fatty acids were C16:1omega7c and C16:0. The major quinone was ubiquinone Q8. Neither methanol dehydrogenase nor methanol oxidase activities were detectable in cells grown on methanol, suggesting an alternative, as-yet unknown, mechanism for methanol oxidation. The isolates assimilated C1 units at the level of formaldehyde, via the serine cycle. The DNA G+C content of the strains ranged between 64 and 65 mol%. 16S rRNA gene sequence similarity between the three new isolates was 99.85-100%, but was below 94% with other members of the Betaproteobacteria, indicating that the isolates represent a novel taxon. Based on physiological, phenotypic and genomic characteristics of the three isolates, a new genus, Methyloversatilis gen. nov., is proposed within the family Rhodocyclaceae. The type strain of Methyloversatilis universalis gen. nov., sp. nov. is FAM5T (=CCUG 52030T=JCM 13912T). 

  • McLaughlin, S. D., Walker, A. W., Churcher, C., Clark, S. K., Tekkis, P. P., Johnson, M. W., Parkhill, J., Ciclitira, P. J., Dougan, G., Nicholls, R. J., & Petrovska, L. (2010). The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing. Annals of Surgery, 252(1), 90–98.

    https://pubmed.ncbi.nlm.nih.gov/17082383/

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Rhodocyclales Family:  Rhodocyclaceae Genus:  Methyloversatilis Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, and contaminated soil
    DNA G+C(%):  64-65
    Opt. T:  30-37℃
    Lower T(℃):  30(+)
    Mid T(℃):  37(+)
    Opt. pH:  7.5-8
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  8.5-9.0(+)
    		Aesculin:  neg Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Fructose:  + Glucose:  + Maltose:  + Ethanol:  + Glycerol:  + Mannitol:  d Methanol:  + Ala:  d Arg:  + Asp:  d Betaine:  + Cys:  d Glu:  d Gly:  + His:  d Met:  + Phe:  d Pro:  d Sarcosine:  d Ser:  + Thr:  d Trp:  d Acetate:  d Citrate:  + Formate:  + Glycolate:  + Malate:  + Pyruvate:  d Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg LeuAA:  + AlkalineP:  neg AcidP:  neg Esterase(C4):  + EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  S(25; disc)
    ampicillin:  R(10; disc)
    penicillin:  R(10; disc)
    cephalothin:  S(30; disc)
    gentamicin:  S(10; disc)
    kanamycin:  S(30; disc)
    neomycin:  S(30; disc)
    streptomycin:  S(10; disc)
    erythromycin:  R(15; disc)
    nalidixic-acid:  S(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(30; disc)
    vancomycin:  R(5; disc)
    chloramphenicol:  R(30; disc)
    SXT:  R(29; disc)

    References

    SPECIFIC REFERENCES FOR METHYLOVERSATILIS UNIVERSALIS
  • Kalyuzhnaya2006 - Methyloversatilis universalis gen. nov., sp. nov., a novel taxon within the Betaproteobacteria represented by three methylotrophic isolates.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR METHYLOVERSATILIS UNIVERSALIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • Added: April 08, 2021