Microbacterium schleiferi

(aka Aureobacterium schleiferi)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Microbacterium schleiferi, (aka Aureobacterium schleiferi), is a Gram-positive, non-spore-forming, strictly aerobic, non-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 66.9%. Microbacterium schleiferi is likely to be transient and not a long-term gut coloniser. (Takeuchi1998; Suzuki2012Bergey; Yokota1993a)



  • This organism has been recovered from human faeces, clinical samples (blood - CCUG) and soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Is a rare opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Borderline cold mesophile, with optimum growth temp of 28C. Unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Takeuchi1998); (Suzuki2012Bergey); (Yokota1993a);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • tolerates 2% salt;
  • 🌡
  • Temperature tolerance:
  • grows weakly at 37℃; Grows optimally at 28℃.
  • Active enzymes:
  • catalase; esterase C4; esterase lipase C8; β-galactosidase; α-glucosidase; β-glucosidase; Leu arylamidase;
    • SPECIAL FEATURES (Takeuchi1998); (Suzuki2012Bergey); (Yokota1993a);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✗
  • Methyl red test:
  • not active
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • yellow

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Micrococcales Family:  Microbacteriaceae Genus:  Microbacterium Alt. name:  Aureobacterium schleiferi Gram stain:  + O2 Relation.:  strictly aerobic Spore:  No spore Motility:  Sessile Morphology:  Rod Pigment:  yellow
    Health:  Unknown
    Source:  human faeces, clinical samples (blood - CCUG) and soil
    DNA G+C(%):  66.9
    Opt. T:  28℃
    Mid T(℃):  37(w)
    NaCl 0.5-2%:  2(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Starch:  neg Tween:  20(d) 40(+) 60(d) 80(d)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg Ribose:  neg Xylose:  neg Lactose:  neg Maltose:  neg Glycogen:  neg Mannitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg Fructose:  neg Glucose:  neg Mannose:  neg Rhamnose:  neg Sorbose:  neg Lactose:  neg Sucrose:  neg Adonitol:  neg Dulcitol:  neg Erythritol:  neg Ethanol:  neg Inulin:  neg Methanol:  neg Salicin:  neg Sorbitol:  neg Hippurate:  neg Acetate:  neg Citrate:  neg Formate:  neg Fumarate:  neg DL-Lactate:  neg Malate:  neg Propionate:  neg Succinate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg LeuAA:  + PyrrolidAA:  neg AlkalineP:  neg AcidP:  neg Esterase(C4):  + EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  yellow

    References

    SPECIFIC REFERENCES FOR MICROBACTERIUM SCHLEIFERI
  • Takeuchi1998 - Union of the genera Microbacterium Orla-Jensen and Aureobacterium Collins et al. in a redefined genus Microbacterium.
  • Suzuki2012Bergey - Bergey's manual of systematic bacteriology. Vol. 5, The Actinobacteria. Part A & B. Microbacteriaceae, Genus I. Microbacteria
  • Yokota1993a - Proposal of six new species in the genus Aureobacterium and transfer of Flavobacterium esteraromaticum Omelianski to the genus Aureobacterium as Aureobacterium esteraromaticum comb. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MICROBACTERIUM SCHLEIFERI
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021