General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview


  • Mixta calida is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, coccobacillus bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 57.4%. Mixta calida is probably a rare gut coloniser. (Palmer2018; Popp2010)



  • This organism has been recovered from infant formula, clinical samples (wound, blood - CCUG) and human faeces (infant - CCUG). Could be a possible pathogen in humans, but unknown at this stage. A possible gut commensal.

  • GENERAL CHARACTERISTICS (Palmer2018); (Popp2010);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 10℃; grows at 37℃; grows at 44℃;
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; fructose; galactose; glucose; mannose; rhamnose; ribose; xylose; cellubiose; gentiobiose; lactose; melibiose; trehalose; glycerol; inositol; mannitol; sorbitol; arbutin; gluconate; 2-ketogluconate; 5-ketogluconate; N-Ac glucosamine; salicin;
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; L-arabinose; cellubiose; aesculin; fructose; galactose; gluconate; glucosamine; gentiobiose; glucose; glycerol; inositol; lactose; maltose; maltotriose; mannitol; mannose; melibiose; rhamnose; ribose; raffinose; sorbitol; sucrose; trehalose; xylose; alanine; D-alanine; aspartate; glutamate; proline; serine; aconitate; citrate; fumarate; DL-glycerate; 2-ketogluconate; 5-ketogluconate; 2-ketoglutarate; DL-lactate; L-malate; mucate; saccharate; succinate; methyl-β-glucoside; methyl-β-galactoside; lactulose; glutamine; D-glucuronate;
  • Active enzymes:
  • catalase; β-galactosidase; β-glucosidase;

  • SPECIAL FEATURES (Palmer2018); (Popp2010);
    Character Response
  • Metabolites not produced:
  • H₂S; indole;
  • VP test:
  • active
  • Pigments:
  • not produced

  • Details


    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Enterobacterales Family:  Erwiniaceae Genus:  Mixta Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Coccobacillus Pigment:  neg
    Health:  Unknown
    Source:  infant formula, clinical samples (wound, blood - CCUG) and human faeces (infant - CCUG)
    DNA G+C(%):  57.4
    Low T(℃):  10(neg)
    Mid T(℃):  37(+)
    High T(℃):  44(+)
    Aesculin:  + Gelatin:  neg

    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  + Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  neg Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  + Gentiobiose:  + Lactose:  + Melezitose:  neg Melibiose:  + Trehalose:  + Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  + Inositol:  + Mannitol:  + Sorbitol:  + Xylitol:  neg Arbutin:  + Gluconate:  + 2-Ketogluconate:  + 5-Ketogluconate:  + Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  + Salicin:  +

    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  + Fructose:  + Fucose:  neg Galactose:  + Glucose:  + D-Lyxose:  neg Mannose:  + Rhamnose:  + Ribose:  + Sorbose:  neg D-Tagatose:  neg Xylose:  + Cellubiose:  + Gentiobiose:  + Lactose:  + Maltose:  + Maltotriose:  + Melezitose:  neg Melibiose:  + Raffinose:  + Sucrose:  + D-Turanose:  neg Trehalose:  + N_Acetyl_glucosamine:  + Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Aesculin:  + Ethanolamine:  neg Gluconate:  + Glucosamine:  + Glycerol:  + Inositol:  + Mannitol:  + Me-α-D-Glc:  neg Sorbitol:  + Xylitol:  neg Ala:  + D-Ala:  + 4-Aminobutyrate:  neg 5-Aminovalerate:  neg Asp:  + Betaine:  neg Glu:  + Histamine:  neg His:  neg Pro:  + Ser:  + Trigonelline:  neg Tryptamine:  neg Trp:  neg Tyr:  neg Aconitate:  + Benzoate:  neg Caprate:  neg Caprylate:  neg Citrate:  + Fumarate:  + Glutarate:  neg DL-Glycerate:  + 3-Hydroxybutyrate:  neg 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Itaconate:  neg 2-Ketogluconate:  + 5-Ketogluconate:  + DL-Lactate:  + L-Malate:  + Malonate:  neg Mucate:  + Phenylacetate:  neg Phenylpropionate:  neg Propionate:  neg Quinate:  neg Saccharate:  + Succinate:  + D-Tartrate:  neg L-Tartrate:  neg

    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg β-Galactosidase:  + β-Glucosidase:  + β-Glucuronidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg TrpAA:  neg AspAA:  neg Lipase:  neg

    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    H2S:  neg Indole:  neg Pigment:  neg

    References


    SPECIFIC REFERENCES FOR MIXTA CALIDA
  • Palmer2018 - Mixta gen. nov., a new genus in the Erwiniaceae.
  • Popp2010 - Pantoea gaviniae sp. nov. and Pantoea calida sp. nov., isolated from infant formula and an infant formula production environment.
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  • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MIXTA CALIDA
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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  • GENERAL REFERENCES FOR MIXTA CALIDA
  • CCUG - Culture Collection University of Gothenburg - Entire Collection