Mycobacterium fortuitum

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Mycobacterium fortuitum is a Gram-variable, non-spore-forming, aerobic, non-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 66.4%. Mycobacterium fortuitum is probably a rare gut coloniser. (Tsukamura1966; Magee2012Bergey)



  • This organism has been recovered from human faeces and clinical sources (sputum, blood, abscess - CCUG). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Tsukamura1966); (Magee2012Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • urea;
  • 🧂
  • Salt tolerance:
  • tolerates 5(d)% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 43℃;
  • H+
  • Acid from carbohydrates usually produced:
  • glucose; mannose;
  • ⥅
  • Substrates assimilated or utilised:
  • cellubiose; fructose; gluconate; glucose; inositol; mannitol; rhamnose; sorbitol; trehalose; xylose; γ-aminobutyrate; leucine; ornithine; acetate; fumarate; L-malate; phenylacetate; propionate; pyruvate; putrescine;
  • Active enzymes:
  • esterase C4; esterase lipase C8; β-glucosidase; Leu arylamidase; Pro arylamidase; urease;
  • ±
  • Strain-dependent active enzymes:
  • catalase;
    • SPECIAL FEATURES (Tsukamura1966);
    Character Response
  • ✓
  • Nitrate:
  • reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Penicillins:
  • penicillin G;
  • Cephalosporins:
  • ceftazidime;
  • Macrolides:
  • azithromycin; clarithromycin; josamycin; roxithromycin; telithromycin;
  • Tetracyclines:
  • doxycycline; tigecycline;
  • Quinolines:
  • ciprofloxacin; gatifloxacin; levofloxacin;
  • Aminoglycosides:
  • amikacin;
  • Heterocycles:
  • chloramphenicol;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • telithromycin;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Mycobacteriales Family:  Mycobacteriaceae Genus:  Mycobacterium Gram stain:  vr O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Rod Pigment:  neg
    Health:  Unknown
    Source:  human faeces and clinical sources (sputum, blood, abscess - CCUG)
    DNA G+C(%):  66.4
    Lower T(℃):  25(+)
    High T(℃):  43(+)
    NaCl 3-5%:  5(d(+))
    		Aesculin:  vr Urea:  + Gelatin:  neg Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Galactose:  neg Glucose:  + Mannose:  d(+) Rhamnose:  neg Ribose:  neg Xylose:  neg Lactose:  neg Maltose:  neg Sucrose:  neg Trehalose:  neg Glycogen:  neg Dulcitol:  neg Inositol:  neg Mannitol:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  + Galactose:  neg Glucose:  + Mannose:  neg Rhamnose:  + Ribose:  neg Xylose:  + Cellubiose:  + Maltose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  + N_Acetyl_glucosamine:  neg Adonitol:  neg Arbutin:  neg Gluconate:  + Inositol:  + Mannitol:  + Salicin:  neg Sorbitol:  + Ala:  neg β-Ala:  neg 4-Aminobutyrate:  + Asp:  neg His:  neg Leu:  + Orn:  + Phe:  neg Pro:  neg Ser:  neg Tyr:  neg Acetate:  + Aconitate:  cis(neg) Adipate:  neg Azelate:  neg Citrate:  neg Fumarate:  + Glutarate:  neg 3-Hydroxybutyrate:  neg 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Itaconate:  neg DL-Lactate:  neg L-Malate:  + Mesaconate:  neg Phenylacetate:  + Propionate:  + Pyruvate:  + Suberate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  d Urease:  + Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  vr β-Glucosidase:  + β-Glucuronidase:  neg α-Mannosidase:  neg GluDC:  neg LeuAA:  + ProAA:  + PyrrolidAA:  neg AlkalineP:  neg AcidP:  vr Esterase(C4):  + EstLip(C8):  + Lipase(C14):  vr
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    penicillin_G:  R(>32)
    ceftazidime:  R(>256)
    amikacin:  S(1)
    azithromycin:  R(MIC50): >64, MIC90: >64, RNG: (1->64)
    clarithromycin:  R(MIC50): 16, MIC90: 64, RNG: (≥0.03->64)
    roxithromycin:  R(MIC50): >64, MIC90: >64, RNG: (0.12->64)
    telithromycin:  R(MIC50): >64, MIC90: >64, RNG: (0.12->64)
    josamycin:  R(MIC50): >64, MIC90: >64, RNG: (4->64)
    ciprofloxacin:  S(MIC50): 0.023), MIC90: S(0.023, RNG: (1-64)
    gatifloxacin:  S(MIC50): ≤0.12, MIC90: ≤0.12, RNG: (≤0.12-0.5)
    levofloxacin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (≤0.03-1)
    sparfloxacin:  RNG: (0.06-)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(MIC50): 0.19), MIC90: S(0.19, RNG: (≤0.25->128)
    minocycline:  RNG: (≤0.12->64)
    tigecycline:  SensRNG: (<0.06-≤0.25)
    vancomycin:  R(32)
    rifampicin:  RNG: (8-)
    chloramphenicol:  R(>256)
    isoniazid:  RNG: (1-2)

    References

    SPECIFIC REFERENCES FOR MYCOBACTERIUM FORTUITUM
  • Tsukamura1966 - Adansonian Classification of Mycobacteria.
  • Magee2012Bergey - Bergey's manual of systematic bacteriology. Vol. 5, The Actinobacteria. Part A & B. Mycobacteriaceae, Genus I. Bifidobacterium
  • Schinsky2004 - Taxonomic variation in the Mycobacterium fortuitum third biovariant complex: description of Mycobacterium boenickei sp. nov., Mycobacterium houstonense sp. nov., Mycobacterium neworleansense sp. nov. and Mycobacterium brisbanense sp. nov. and recognition of Mycobacterium porcinum from human clinical isolates.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR MYCOBACTERIUM FORTUITUM
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021