Negativibacillus massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Negativibacillus massiliensis is a Gram-negative, spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 45.4%. Negativibacillus massiliensis is probably a common, although minor, coloniser of the gut. (Ricaboni2016b; Valles2021)



  • This organism has been recovered from human left colon and human faeces (Bilen2018e). Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • In 53% of Europeans (unseenbio.com). Minor coloniser.
    • GENERAL CHARACTERISTICS (Ricaboni2016b); (Valles2021);
    Character Response
  • pH
  • Acidity tolerance:
  • tolerates pH 5.0; tolerates pH 8.0; Grows optimally at pH 7.5.
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 28℃; grows at 37℃; grows at 45℃;
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; glucose; ribose; D-tagatose; gentiobiose; lactose; D-lyxose; trehalose; D-arabitol; L-arabitol; dulcitol; mannitol; xylitol; arbutin; 5-ketogluconate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; chymotrypsin; α-galactosidase; β-galactosidase; Leu arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Valles2021);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
    • RESPONSE TO ANTIBIOTICS (Valles2021);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; amoxicillin-clavulanic acid; imipenem; oxacillin; penicillin G;
  • Cephalosporins:
  • cefotaxime;
  • cefalexin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline;
  • Aminoglycosides:
  • tobramycin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • fosfomycin; metronidazole; trimethoprim-sulfamethoxazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • daptomycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Negativicutes Family:  Firmicutes sensu stricto incertae sedis Genus:  Negativibacillus Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  Endospore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human left colon and human faeces (Bilen2018e)
    DNA G+C(%):  45.4
    Lower T(℃):  28(neg)
    Mid T(℃):  37(+)
    High T(℃):  45(+)
    Opt. pH:  7.5
    pH 4.2-5.9:  5.0(+)
    pH 6.0-8.0:  8.0(+)
    		Urea:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  + Glucose:  + D-Lyxose:  + Ribose:  + D-Tagatose:  + Gentiobiose:  + Lactose:  + Trehalose:  + D-Arabitol:  + L-Arabitol:  + Dulcitol:  + Mannitol:  + Xylitol:  + Arbutin:  + 5-Ketogluconate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  + β-Galactosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg Chymotrypsin:  + Trypsin:  neg CystineAA:  neg LeuAA:  + ValAA:  neg AlkalineP:  + AcidP:  + Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  R(30; disc)
    Augmentin:  R(30; disc)
    oxacillin:  R(5; disc)
    penicillin_G:  R(1U)
    imipenem:  R(10; disc)
    cefalexin:  R(15)
    cefotaxime:  S(50; disc)
    tobramycin:  R(10; disc)
    erythromycin:  R(15; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  R(30; disc)
    vancomycin:  R(30; disc)
    rifampicin:  R(30; disc)
    fosfomycin:  R(50; disc)
    metronidazole:  R(4; disc)
    SXT:  R(25; disc)
    daptomycin:  R(15; disc)

    References

    SPECIFIC REFERENCES FOR NEGATIVIBACILLUS MASSILIENSIS
  • Ricaboni2016b - "Negativibacillus massiliensis" gen. nov., sp. nov., isolated from human left colon.
  • Valles2021 - Negativibacillus massiliensis gen. nov., sp. nov., a New Bacterial Genus Isolated from a Human Left Colon Sample
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR NEGATIVIBACILLUS MASSILIENSIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • ...............................
    • GENERAL REFERENCES FOR NEGATIVIBACILLUS MASSILIENSIS
  • Bilen2018e - Description of the human gut microbiota by culturomics
    • Added: January 06, 2022