Neobacillus fumarioli

(aka Bacillus fumarioli)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Neobacillus fumarioli, (aka Bacillus fumarioli), is a Gram-positive, spore-forming, strictly aerobic, motile, rod-shaped bacterium. The DNA G+C content is 40.7%. Neobacillus fumarioli is likely to be transient and not a long-term gut coloniser. (Logan2000a)



  • This organism has been recovered from geothermal soils and active and inactive fumaroles, Antarctica. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe and high optimum temperature suggest it is not a gut coloniser. Also requires low pH to grow.
    • GENERAL CHARACTERISTICS (Logan2000a);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; gelatin;
  • pH
  • Acidity tolerance:
  • tolerates pH 4.0; tolerates pH 6.5; Grows optimally at pH 5.5.
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 55℃; Grows optimally at 50℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; sucrose; mannitol;
  • ±
  • Strain-dependent acid from carbs:
  • galactose; ribose; lactose; maltose; melezitose; melibiose; raffinose; D-turanose; glycerol; α-methyl glucoside;
  • Active enzymes:
  • catalase;
    • SPECIAL FEATURES (Logan2000a);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Logan2000a);
    Class Active Resistant
  • Penicillins:
  • ampicillin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • kanamycin; streptomycin;
  • Heterocycles:
  • chloramphenicol; nitrofurantoin;
  • Miscellaneous antibiotics:
  • colistin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Neobacillus Alt. name:  Bacillus fumarioli Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  geothermal soils and active and inactive fumaroles, Antarctica
    DNA G+C(%):  40.7
    Opt. T:  50℃
    Lower T(℃):  30(+)
    High T(℃):  55(+)
    Opt. pH:  5.5
    pH 4.2-5.9:  4.0(+)
    pH 6.0-8.0:  6.5(+)
    		Aesculin:  + Gelatin:  + Casein:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  d Glucose:  + Mannose:  + D-Lyxose:  neg Rhamnose:  neg Ribose:  d Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  d Maltose:  d Melezitose:  d Melibiose:  d Sucrose:  + Trehalose:  w Turanose:  d Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  d Inositol:  neg Mannitol:  + Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  d Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  w Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  + Urease:  neg β-Galactosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg TrpAA:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(25; disc)
    kanamycin:  S(30; disc)
    streptomycin:  S(25; disc)
    nalidixic-acid:  R(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(100; disc)
    chloramphenicol:  S(50; disc)
    nitrofurantoin:  S(50; disc)
    colistin:  S(100; disc)

    References

    SPECIFIC REFERENCES FOR NEOBACILLUS FUMARIOLI
  • Zheng2020a - Specific gut microbiome signature predicts the early-stage lung cancer
  • Logan2000a - Aerobic endospore-forming bacteria from geothermal environments in northern Victoria Land, Antarctica, and Candlemas Island, South Sandwich archipelago, with the proposal of Bacillus fumarioli sp. nov.
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    • Added: September 19, 2022