Neobacillus niacini

(aka Bacillus niacini)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Neobacillus niacini, (aka Bacillus niacini), is a Gram-positive, spore-forming, aerobic, variably-motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 37-39%. Neobacillus niacini is probably a rare gut coloniser. (Logan2011Bergey; Nagel1991)



  • This organism has been recovered from human faeces, and soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal. Robust growth can have positive consequences for gut health.
    • GENERAL CHARACTERISTICS (Logan2011Bergey); (Nagel1991);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • starch; urea;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 2.0% salt; doesn't tolerate 5.0% salt; doesn't tolerate 7.0% salt;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.0; tolerates pH 6.0-8.0; doesn't tolerate pH 9.0; Grows optimally at pH 7.
  • 🌡
  • Temperature tolerance:
  • grows at 10℃; grows at 40℃; doesn't grow at 50℃;
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; rhamnose; ribose; xylose; amygdalin; glycogen; inulin; starch; cellubiose; gentiobiose; lactose; maltose; melezitose; melibiose; raffinose; sucrose; trehalose; dulcitol; inositol; sorbitol; xylitol; arbutin; methyl mannoside; methyl xyloside; N-Ac glucosamine; salicin;
  • ⥅
  • Substrates assimilated or utilised:
  • glycerol; melibiose; glutamate; acetate; fumarate; lactate; malate; pyruvate; succinate; glutamine;
  • ±
  • Strain-dependent substrate utilisation:
  • aspartate; citrate; formate;
  • Active enzymes:
  • catalase; β-galactosidase; oxidase;
    • SPECIAL FEATURES (Logan2011Bergey); (Nagel1991);
    Character Response
  • ✓
  • Metabolites produced:
  • indole (strain-dependent);
  • ✗
  • Metabolites not produced:
  • H₂S;
  • ✗
  • VP test:
  • not active
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Caryophanales Family:  Bacillaceae Genus:  Neobacillus Alt. name:  Bacillus niacini Gram stain:  + O2 Relation.:  aerobic Spore:  Endospore Motility:  vr Morphology:  Rod
    Health:   Positive
    Source:  human faeces, and soil
    DNA G+C(%):  37-39
    Low T(℃):  10(+)
    Mid T(℃):  40(+)
    High T(℃):  50(neg)
    NaCl 0.5-2%:  2.0(neg)
    NaCl 3-5%:  5.0(neg)
    NaCl >6%:  7.0(neg)
    Opt. pH:  7
    pH 4.2-5.9:  5.0(neg)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  9.0(neg)
    		Urea:  d Gelatin:  + Starch:  d Casein:  neg Tyrosine:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  neg Rhamnose:  d(+) Ribose:  d(+) D-Tagatose:  neg Xylose:  + L-Xylose:  neg Cellubiose:  d(+) Gentiobiose:  d(+) Lactose:  d(+) Maltose:  + Melezitose:  d(+) Melibiose:  + Sucrose:  + Trehalose:  + Amygdalin:  + Glycogen:  d(+) Inulin:  d(+) Starch:  + Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  d(+) Erythritol:  neg Glycerol:  neg Inositol:  d(+) Mannitol:  neg Sorbitol:  d(+) Xylitol:  d(+) Arbutin:  d(+) Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Mann:  d(+) Me-Xyloside:  d(+) NAc-α-GA:  + Salicin:  d(+)
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Melibiose:  + Glycerol:  + Asp:  d Glu:  + Acetate:  + Citrate:  d Formate:  d Fumarate:  + Lactate:  + Malate:  + Pyruvate:  + Succinate:  + D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg β-Galactosidase:  + ArgDH:  neg LysDC:  neg OrnDC:  neg TrpAA:  neg Lecithinase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  (d)

    References

    SPECIFIC REFERENCES FOR NEOBACILLUS NIACINI
  • Logan2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Bacillaceae Genus I. Bacillus
  • Nagel1991 - Bacillus niacini sp. nov., a Nicotinate-Metabolizing Mesophile Isolated from Soil.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR NEOBACILLUS NIACINI
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR NEOBACILLUS NIACINI
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021