Noviherbaspirillum massiliense

(aka Herbaspirillum massiliense)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Noviherbaspirillum massiliense, (aka Herbaspirillum massiliense), is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped - curved bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 59.7%. Noviherbaspirillum massiliense is probably a rare gut coloniser. (Chaudhary2017; Ishii2017; Lagier2012d)



  • This organism has been recovered from human faeces. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal. Robust growth can have positive consequences for gut health.
    • GENERAL CHARACTERISTICS (Chaudhary2017); (Ishii2017); (Lagier2012d);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • tolerates 1% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0-8.0; tolerates pH 9; Grows optimally at pH 7.5-8.0.
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 37℃; doesn't grow at 45℃; Grows optimally at 28-37℃.
  • ⥅
  • Substrates assimilated or utilised:
  • acetate; butyrate; 3-hydroxybutyrate; lactate; suberate; succinate;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; arginine dihydrolase; catalase; esterase C4; esterase lipase C8; Leu arylamidase; oxidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Chaudhary2017); (Ishii2017); (Lagier2012d);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S; indole;
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • light brown
    • RESPONSE TO ANTIBIOTICS (Lagier2012d);
    Class Active Resistant
  • Penicillins:
  • imipenem; ticarcillin;
  • Aminoglycosides:
  • amikacin; gentamicin;
  • Heterocycles:
  • metronidazole; trimethoprim-sulfamethoxazole;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Oxalobacteraceae Genus:  Noviherbaspirillum Alt. name:  Herbaspirillum massiliense Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod - curved Pigment:  light brown
    Health:   Positive
    Source:  human faeces
    DNA G+C(%):  59.7
    Opt. T:  28-37℃
    Lower T(℃):  30(+)
    Mid T(℃):  37(+)
    High T(℃):  45(neg)
    NaCl 0.5-2%:  1(+)
    Opt. pH:  7.5-8.0
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  9(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Casein:  neg Chitin:  neg DNA:  neg Tyrosine:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Fructose:  neg Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg Rhamnose:  neg Ribose:  neg Xylose:  neg Lactose:  neg Maltose:  neg Melibiose:  neg Sucrose:  neg N_Acetyl_glucosamine:  neg Ethanol:  neg Gluconate:  neg Glycerol:  neg Glycogen:  neg Inositol:  neg Mannitol:  neg Salicin:  neg Sorbitol:  neg Ala:  neg Arg:  neg His:  neg Pro:  neg Ser:  neg Trp:  neg Acetate:  + Adipate:  neg Butyrate:  + Caprate:  neg Citrate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg Itaconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Lactate:  + Malate:  neg Malonate:  neg Phenylacetate:  neg Propionate:  neg Pyruvate:  neg Suberate:  + Succinate:  + Valerate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  + Chymotrypsin:  neg Trypsin:  neg CystineAA:  neg LeuAA:  + ValAA:  neg AlkalineP:  + AcidP:  + Esterase(C4):  + EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg Pigment:  light brown
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ticarcillin:  Sens
    imipenem:  Sens
    amikacin:  Sens
    gentamicin:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    metronidazole:  Sens
    SXT:  Sens

    References

    SPECIFIC REFERENCES FOR NOVIHERBASPIRILLUM MASSILIENSE
  • Chaudhary2017 - Noviherbaspirillum agri sp. nov., isolated from reclaimed grassland soil, and reclassification of Herbaspirillum massiliense (Lagier et al., 2014) as Noviherbaspirillum massiliense comb. nov.
  • Ishii2017 - Noviherbaspirillum denitrificans sp. nov., a denitrifying bacterium isolated from rice paddy soil and Noviherbaspirillum autotrophicum sp. nov., a denitrifying, facultatively autotrophic bacterium isolated from rice paddy soil and proposal to reclassify Herbaspirillum massiliense as Noviherbaspirillum massiliense comb. nov.
  • Lagier2012d - Non-contiguous finished genome sequence and description of Herbaspirillum massiliense sp. nov.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR NOVIHERBASPIRILLUM MASSILIENSE
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021