Oceanobacillus manasiensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Oceanobacillus manasiensis is a Gram-positive, spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 39.7%. Oceanobacillus manasiensis is probably a rare gut coloniser. (Wang2010a)



  • This organism has been recovered from salt lakes, China. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • Apparently aerobic but lacks catalase and oxidase activities.
    • GENERAL CHARACTERISTICS (Wang2010a);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 2% salt; tolerates 2.5% salt; tolerates 15% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0; tolerates pH 11; Grows optimally at pH 8-10.
  • 🌡
  • Temperature tolerance:
  • grows at 10℃; grows at 42℃; Grows optimally at 30-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; rhamnose;
  • Active enzymes:
  • β-galactosidase;
    • SPECIAL FEATURES (Wang2010a);
    Character Response
  • ✗
  • Metabolites not produced:
  • H₂S;
  • ✗
  • VP test:
  • not active
  • ✓
  • Nitrate:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Wang2010a);
    Class Active Resistant
  • Penicillins:
  • ampicillin;
  • Macrolides:
  • erythromycin;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • spectinomycin; streptomycin;
  • gentamicin; kanamycin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • chloramphenicol;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Caryophanales Family:  Bacillaceae Genus:  Oceanobacillus Gram stain:  + O2 Relation.:  aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  salt lakes, China
    DNA G+C(%):  39.7
    Opt. T:  30-37℃
    Low T(℃):  10(+)
    High T(℃):  42(+)
    NaCl 0.5-2%:  2(neg)
    NaCl 3-5%:  2.5(+)
    NaCl >6%:  15(+)
    Opt. pH:  8-10
    pH 6.0-8.0:  6.0(+)
    pH >8:  11(+)
    		Aesculin:  + Urea:  neg Gelatin:  neg Casein:  neg Tyrosine:  neg Tween:  20(+) 80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + Mannose:  + Rhamnose:  + Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Citrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg β-Galactosidase:  + ArgDH:  neg LysDC:  neg OrnDC:  neg TrpAA:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(100; disc)
    gentamicin:  R(40; disc)
    kanamycin:  R(50; disc)
    spectinomycin:  S(50; disc)
    streptomycin:  S(200; disc)
    erythromycin:  S(30; disc)
    nalidixic-acid:  R(20; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    rifampicin:  S(5; disc)
    chloramphenicol:  S(20; disc)

    References

    SPECIFIC REFERENCES FOR OCEANOBACILLUS MANASIENSIS
  • Wang2010a - Oceanobacillus manasiensis sp. nov., a moderately halophilic bacterium isolated from the salt lakes of Xinjiang, China.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR OCEANOBACILLUS MANASIENSIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • ...............................
    • Added: January 14, 2022