Pectobacterium wasabiae

(aka Pectobacterium carotovorum subsp. wasabiae)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Pectobacterium wasabiae, (aka Pectobacterium carotovorum subsp. wasabiae), is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 51.4-51.7%. Pectobacterium wasabiae is likely to be transient and not a long-term gut coloniser. (Gardan2003; Hauben1998)



  • This organism has been recovered from rotting horseradish, Japan. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: plant pathogen). Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Low optimum temperatures (27-30C) and inability to grow at 37C makes this an unlikely gut coloniser.
    • GENERAL CHARACTERISTICS (Gardan2003); (Hauben1998);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; casein; gelatin;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 5% salt;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 36℃; Grows optimally at 27-30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; ribose; gentiobiose; lactose; sucrose; trehalose; mannitol; N-Ac glucosamine; salicin;
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; arabinose; arbutin; cellubiose; fructose; galactose; gluconate; glucosamine; glucose; glycerol; inositol; mannitol; mannose; rhamnose; ribose; raffinose; salicin; γ-aminobutyrate; arginine; L-citrulline; glycine; histidine; leucine; methionine; phenylalanine; serine; tryptophan; tyrosine; citrate; fumarate; 2-ketogluconate; malate; succinate; meso-tartrate; methyl-β-glucoside; L-asparagine; D-glucuronate; NH4+ consumed;
  • Active enzymes:
  • catalase;
    • SPECIAL FEATURES (Gardan2003); (Hauben1998);
    Character Response
  • ✓
  • Metabolites produced:
  • H₂S;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✓
  • Methyl red test:
  • active
  • ✗
  • VP test:
  • not active

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Enterobacterales Family:  Pectobacteriaceae Genus:  Pectobacterium Alt. name:  Pectobacterium carotovorum subsp. wasabiae Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  rotting horseradish, Japan
    DNA G+C(%):  51.4-51.7
    Opt. T:  27-30℃
    Mid T(℃):  36(neg)
    NaCl 3-5%:  5(neg)
    		Aesculin:  + Gelatin:  + Starch:  neg Casein:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  + Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  neg Gentiobiose:  + Lactose:  + Maltose:  neg Melibiose:  neg Sucrose:  + Trehalose:  + Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg Inositol:  neg Mannitol:  + Sorbitol:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-Xyloside:  neg NAc-α-GA:  + Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + Fructose:  + Galactose:  + Glucose:  + D-Lyxose:  neg Mannose:  + Rhamnose:  + Ribose:  + Sorbose:  neg Cellubiose:  + Raffinose:  + Sucrose:  neg N_Acetyl_glucosamine:  + Adonitol:  neg D-Arabitol:  neg Arbutin:  + Gluconate:  + Glucosamine:  + Glycerol:  + Inositol:  + Mannitol:  + Methanol:  neg Salicin:  + Sorbitol:  neg Xylitol:  neg Ala:  neg 4-Aminobutyrate:  + Arg:  + Betaine:  neg L-Citrulline:  + Glu:  neg Gly:  + His:  + DL-Kynurenine:  neg Leu:  + Met:  + Phe:  + Sarcosine:  neg Ser:  + Trigonelline:  neg Tryptamine:  neg Trp:  + Tyr:  + Adipate:  neg Benzoate:  neg Citrate:  + Fumarate:  + 2-Ketogluconate:  + Malate:  + Malonate:  neg Propionate:  neg Succinate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  neg β-Galactosidase:  neg ArgDH:  neg ArgDC:  neg LysDC:  neg OrnDC:  neg Phe deaminase:  neg TrpAA:  neg Lecithinase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  + Indole:  neg

    References

    SPECIFIC REFERENCES FOR PECTOBACTERIUM WASABIAE
  • Gardan2003 - Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov.
  • Hauben1998 - Phylogenetic position of phytopathogens within the Enterobacteriaceae.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PECTOBACTERIUM WASABIAE
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
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    • Added: May 15, 2022