Peptoniphilus harei

(aka Peptostreptococcus harei)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Peptoniphilus harei, (aka Peptostreptococcus harei), is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, coccus bacterium. It has been detected in at least 9 gut microbiome compilation studies or metastudies. The DNA G+C content is 25%. Peptoniphilus harei is probably a common, although minor, coloniser of the gut. (Ezaki2001; Murdoch1997; Ezaki2011eBergey)



  • This organism has been recovered from clinical sources (sacral ulcer) and human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Ezaki2001); (Murdoch1997); (Ezaki2011eBergey);
    Character Response
  • Active enzymes:
  • acid phosphatase; Arg arylamidase; catalase;
    • SPECIAL FEATURES (Ezaki2001); (Murdoch1997); (Ezaki2011eBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate (minor); butyrate; valerate (minor); isovalerate (minor);
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Goldstein2018a); (Goldstein2013b); (Tyrrell2012); (Goldstein2006a); (Goldstein2006b);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin; ampicillin-sulbactam; ertapenem; imipenem; meropenem; penicillin; penicillin G; piperacillin-tazobactam;
  • Cephalosporins:
  • cefepime; cefotaxime; cefotetan; cefoxitin; ceftazidime;
  • Tetracyclines:
  • tigecycline;
  • tetracycline;
  • Quinolines:
  • moxifloxacin;
  • Heterocycles:
  • metronidazole;
  • Vancomycins:
  • vancomycin; dalbavancin;
  • Miscellaneous antibiotics:
  • clindamycin; daptomycin; linezolid;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Tissierellia Order:  Tissierellales Family:  Peptoniphilaceae Genus:  Peptoniphilus Alt. name:  Peptostreptococcus harei Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus
    Health:  Unknown
    Source:  clinical sources (sacral ulcer) and human faeces
    DNA G+C(%):  25
    		Urea:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  vr Glucose:  neg Mannose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Sucrose:  neg Trehalose:  neg Mannitol:  neg Sorbitol:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  + Urease:  neg Coagulase:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  neg ArgAA:  + GluGluAA:  neg GlyAA:  neg HisAA:  vr LeuAA:  neg LeuGlyAA:  neg PyrrolidAA:  neg TyrAA:  vr AlkalineP:  neg AcidP:  + Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Propionate:  minor(+) Butyrate:  + Valerate:  minor(+) Isovalerate:  minor(+) Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 0.023, MIC90: 0.25, RNG: (0.016-0.38)
    ampicillin:  S(MIC50): 0.03, MIC90: 0.25, RNG: (0.03–>8)
    amp-sulb:  S(MIC50): 0.125, MIC90: 0.25, RNG: (0.06–0.25)
    penicillin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.03–0.5)
    penicillin_G:  S(MIC50): 0.023, MIC90: 0.032, RNG: (0.016-0.19)
    piper-taz:  S(MIC50): 0.03, MIC90: 0.125, RNG: (0.015–0.25)
    ertapenem:  S(MIC50): 0.012, MIC90: 0.016, RNG: (0.006-0.023)
    imipenem:  S(MIC50): 0.06, MIC90: 0.125, RNG: (0.015–0.25)
    meropenem:  S(MIC50): 0.008, MIC90: 0.032, RNG: (0.004-0.032)
    cefepime:  S(MIC50): 0.5, MIC90: 4, RNG: (0.25–8)
    cefotaxime:  S(MIC50): 0.25, MIC90: 2, RNG: (0.06–2)
    cefotetan:  S(MIC50): 0.5, MIC90: 1, RNG: (0.38-8)
    cefoxitin:  S(MIC50): 0.094, MIC90: 0.5, RNG: (0.023-1.5)
    ceftazidime:  S(MIC50): 1, MIC90: 1, RNG: (0.5–1)
    erythromycin:  Var
    linezolid:  S(MIC50): 0.75, MIC90: 1.5, RNG: (0.5-2)
    ciprofloxacin:  RNG: (0.5-8)
    levofloxacin:  Var(MIC50): 2, MIC90: >8, RNG: (0.125–>8)
    moxifloxacin:  S(MIC50): 0.19, MIC90: 0.38, RNG: (0.125-1.5)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  Var(MIC50): 8, MIC90: 16, RNG: (0.064-24)
    tetracycline:  Res
    tigecycline:  S(MIC50): 0.094, MIC90: 0.25, RNG: (0.023-0.25)
    dalbavancin:  S(MIC50): 0.03, MIC90: 0.125, RNG: (0.015–0.25)
    vancomycin:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.06–0.5)
    chloramphenicol:  Var(MIC50): 3, MIC90: 3, RNG: (1.5-4)
    metronidazole:  S(MIC50): 0.5, MIC90: 1, RNG: (0.25–2)
    clindamycin:  S(MIC50): 0.25, MIC90: 1.5, RNG: (0.094-1.5)
    daptomycin:  S(MIC50): 0.25, MIC90: 1, RNG: (0.03–1)

    References

    SPECIFIC REFERENCES FOR PEPTONIPHILUS HAREI
  • Ezaki2001 - Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus.
  • Murdoch1997 - Description of Three New Species of the Genus Peptostreptococcus from Human Clinical Specimens: Peptostreptococcus harei sp. nov., Peptostreptococcus ivorii sp. nov., and Peptostreptococcus octavius sp. nov.
  • Ezaki2011eBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Clostridiales, Family XI. Incertae Sedis, Genus VI. Peptoniphilus
  • Goldstein2018a - Comparative In Vitro Activities of Relebactam, Imipenem, the Combination of the Two, and Six Comparator Antimicrobial Agents against 432 Strains of Anaerobic Organisms, Including Imipenem-Resistant Strains.
  • Goldstein2013b - Comparative in vitro activities of GSK2251052, a novel boron-containing leucyl-tRNA synthetase inhibitor, against 916 anaerobic organisms.
  • Tyrrell2012 - In vitro activity of TD-1792, a multivalent glycopeptide-cephalosporin antibiotic, against 377 strains of anaerobic bacteria and 34 strains of Corynebacterium species.
  • Goldstein2006a - In vitro activity of ceftobiprole against aerobic and anaerobic strains isolated from diabetic foot infections.
  • Goldstein2006b - In vitro activities of dalbavancin and 12 other agents against 329 aerobic and anaerobic gram-positive isolates recovered from diabetic foot infections.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PEPTONIPHILUS HAREI
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Jie2017 - The gut microbiome in atherosclerotic cardiovascular disease
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR PEPTONIPHILUS HAREI
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021