Peptoniphilus lacrimalis

(aka Peptostreptococcus lacrimalis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Peptoniphilus lacrimalis, (aka Peptostreptococcus lacrimalis), is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, coccus bacterium. It has been detected in at least 8 gut microbiome compilation studies or metastudies. The DNA G+C content is 30-31%. Peptoniphilus lacrimalis is probably a common, although minor, coloniser of the gut. (Ezaki2001; Ezaki2011eBergey; Li1992; Murdoch1998)



  • This organism has been recovered from clinical sources (abscess, blood, vagina, eye - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Ezaki2001); (Ezaki2011eBergey); (Li1992); (Murdoch1998);
    Character Response
  • Active enzymes:
  • Ala arylamidase; Gly arylamidase; His arylamidase; Leu arylamidase; Phe arylamidase;
    • SPECIAL FEATURES (Ezaki2001); (Ezaki2011eBergey); (Li1992); (Murdoch1998);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate (minor); butyrate; lactate (minor);
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Tyrrell2012);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin; ertapenem; imipenem; meropenem; penicillin; penicillin G; piperacillin-tazobactam;
  • Cephalosporins:
  • cefotetan; cefoxitin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline; tetracycline; tigecycline;
  • Quinolines:
  • moxifloxacin;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; daptomycin; linezolid;

  • Walker, A. W., Sanderson, J. D., Churcher, C., Parkes, G. C., Hudspith, B. N., Rayment, N., Brostoff, J., Parkhill, J., Dougan, G., & Petrovska, L. (2011). High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease. BMC Microbiology, 11, 7.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Tissierellia Order:  Tissierellales Family:  Peptoniphilaceae Genus:  Peptoniphilus Alt. name:  Peptostreptococcus lacrimalis Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus
    Health:  Unknown
    Source:  clinical sources (abscess, blood, vagina, eye - CCUG) and human faeces
    DNA G+C(%):  30-31
    		Urea:  neg Hippurate:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg L-Arabinose:  neg Glucose:  neg Mannose:  neg Ribose:  neg D-Tagatose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Sucrose:  neg Trehalose:  neg Dextrin:  neg Glycogen:  neg D-Arabitol:  neg Mannitol:  neg Sorbitol:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Urease:  neg Coagulase:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg β-Mannosidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  + AlaPheProAA:  neg GluGluAA:  neg GlyAA:  + HisAA:  + LeuAA:  + LeuGlyAA:  neg PyrrolidAA:  neg PheAA:  + AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Propionate:  minor(+) Butyrate:  + Lactate:  minor(+) Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  SensRNG: (0.016-0.25)
    ampicillin:  S(MIC50): 0.03, MIC90: 0.25, RNG: (0.03–>8)
    penicillin:  SensRNG: (0.016-0.125)
    penicillin_G:  Sens
    piper-taz:  S(MIC50): 0.03, MIC90: 0.5, RNG: (0.03–16)
    ertapenem:  SensRNG: (0.002-0.012)
    imipenem:  S(MIC50): 0.03, MIC90: 0.06, RNG: (0.03–2)
    meropenem:  SensRNG: (0.002-0.016)
    cefotetan:  SensRNG: (0.016-0.38)
    cefoxitin:  SensRNG: (0.016-0.25)
    erythromycin:  Sens
    linezolid:  S(MIC50): 0.5, MIC90: 1, RNG: (0.25–2)
    levofloxacin:  RNG: (3-8)
    moxifloxacin:  SensRNG: (0.002-0.38)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  SensRNG: (0.125-4)
    tetracycline:  Sens
    tigecycline:  SensRNG: (0.023-0.25)
    vancomycin:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.06–1)
    chloramphenicol:  SensRNG: (0.75-3)
    metronidazole:  S(MIC50): 0.25, MIC90: 1, RNG: (0.03–2)
    clindamycin:  SensRNG: (0.016-0.38)
    daptomycin:  S(MIC50): 0.125, MIC90: 0.5, RNG: (0.03–1)

    References

    SPECIFIC REFERENCES FOR PEPTONIPHILUS LACRIMALIS
  • Ezaki2001 - Proposal of the genera Anaerococcus gen. nov., Peptoniphilus gen. nov. and Gallicola gen. nov. for members of the genus Peptostreptococcus.
  • Ezaki2011eBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Clostridiales, Family XI. Incertae Sedis, Genus VI. Peptoniphilus
  • Tyrrell2012 - In vitro activity of TD-1792, a multivalent glycopeptide-cephalosporin antibiotic, against 377 strains of anaerobic bacteria and 34 strains of Corynebacterium species.
  • Li1992 - Three New Species of the Genus Peptostreptococcus Isolated from Humans: Peptostreptococcus vaginalis sp. nov., Peptostreptococcus lacrimalis sp. nov., and Peptostreptococcus lactolyticus sp. nov.
  • Murdoch1998 - Gram-Positive Anaerobic Cocci.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PEPTONIPHILUS LACRIMALIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Dubinkina2017 - Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR PEPTONIPHILUS LACRIMALIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021