Phocaeicola massiliensis

(aka Bacteroides massiliensis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Phocaeicola massiliensis, (aka Bacteroides massiliensis), is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 12 gut microbiome compilation studies or metastudies. The DNA G+C content is 49%. Phocaeicola massiliensis is probably a common, although minor, coloniser of the gut. (Song2010Bergeys; Fenner2005; Terekhov2018; Bellali2019a)



  • This organism has been recovered from clinical sources (blood, brain abscess - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Song2010Bergeys); (Fenner2005); (Bellali2019a);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • 💧
  • Bile tolerance:
  • Grows in the presence of bile
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 42℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; aesculin; starch; lactose; maltose; melibiose; raffinose; sucrose; N-Ac glucosamine;
  • Active enzymes:
  • Ala arylamidase; alkaline phosphatase; N-Ac β-glucosaminidase; fucosidase; α-galactosidase; β-galactosidase; α-glucosidase; Leu arylamidase; Leu-Gly arylamidase;
    • SPECIAL FEATURES (Song2010Bergeys); (Fenner2005); (Bellali2019a);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Haemolysis:
  • absent
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Song2010Bergeys); (Fenner2005); (Goldstein2018a);
    Class Active Resistant
  • Aminoglycosides:
  • kanamycin;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • colistin;

  • Walker, A. W., Sanderson, J. D., Churcher, C., Parkes, G. C., Hudspith, B. N., Rayment, N., Brostoff, J., Parkhill, J., Dougan, G., & Petrovska, L. (2011). High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease. BMC Microbiology, 11, 7.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Bacteroidia Order:  Bacteroidales Family:  Bacteroidaceae Genus:  Phocaeicola Alt. name:  Bacteroides massiliensis Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  clinical sources (blood, brain abscess - CCUG) and human faeces
    DNA G+C(%):  49
    Opt. T:  37℃
    Lower T(℃):  25(+)
    High T(℃):  42(+)
    Bile reaction(%):  +
    		Aesculin:  + Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Fucose:  neg Galactose:  + Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  neg Sorbose:  neg Xylose:  neg Cellubiose:  neg Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  + Sucrose:  + Trehalose:  neg Amygdalin:  neg Aesculin:  + Glycogen:  neg Inulin:  neg Starch:  + Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  vr Xylitol:  neg Gluconate:  neg Me-α-D-Glc:  neg NAc-α-GA:  + Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg α-Arab:  neg Ac-β-glcamnd:  + α-Fucosidase:  + α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  + ArgAA:  neg GluGluAA:  vr GlyAA:  neg HisAA:  neg LeuAA:  + LeuGlyAA:  + ProAA:  neg PyrrolidAA:  neg PheAA:  neg PyrogluAA:  neg SerAA:  neg TyrAA:  neg AlkalineP:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    Acetate, Succinate

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    kanamycin:  R(1000; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    vancomycin:  R(5; disc)
    colistin:  R(10; disc)

    References

    SPECIFIC REFERENCES FOR PHOCAEICOLA MASSILIENSIS
  • Wexler2007 - Bacteroides : the Good, the Bad, and the Nitty-Gritty.
  • Song2010Bergeys - Bacteroides. In Bergey's manual of systematic bacteriology: Vol. 4. The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes
  • Gao2020 - Functional Microbiomics Reveals Alterations of the Gut Microbiome and Host Co-Metabolism in Patients With Alcoholic Hepatitis
  • DeAngelis2013 - Fecal microbiota and metabolome of children with autism and pervasive developmental disorder not otherwise specified
  • Gryp2020 - Isolation and Quantification of Uremic Toxin Precursor-Generating Gut Bacteria in Chronic Kidney Disease Patients
  • Li2019c - Gut Microbiota Differs Between Parkinson's Disease Patients and Healthy Controls in Northeast China
  • Malham2019 - The microbiome reflects diagnosis and predicts disease severity in paediatric onset inflammatory bowel disease
  • Shi2019 - Alterations in the intestinal microbiota of patients with severe and active Graves' orbitopathy: a cross-sectional study
  • Fenner2005 - Bacteroides massiliensis sp. nov., isolated from blood culture of a newborn.
  • Petrov2017 - Analysis of Gut Microbiota in Patients with Parkinson's Disease.
  • Terekhov2018 - Ultrahigh-throughput functional profiling of microbiota communities.
  • Bellali2019a - Parabacteroides massiliensis sp. nov., a new bacterium isolated from a fresh human stool specimen.
  • Feng2015 - Gut microbiome development along the colorectal adenoma-carcinoma sequence
  • Huang2019a - Metagenome-wide association study of the alterations in the intestinal microbiome composition of ankylosing spondylitis patients and the effect of traditional and herbal treatment
  • Goldstein2018a - Comparative In Vitro Activities of Relebactam, Imipenem, the Combination of the Two, and Six Comparator Antimicrobial Agents against 432 Strains of Anaerobic Organisms, Including Imipenem-Resistant Strains.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PHOCAEICOLA MASSILIENSIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Hu2019 - The Gut Microbiome Signatures Discriminate Healthy From Pulmonary Tuberculosis Patients
  • Jie2017 - The gut microbiome in atherosclerotic cardiovascular disease
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Rothschild2018 - Environment dominates over host genetics in shaping human gut microbiota.
  • Urban2020 - Altered Fecal Microbiome Years after Traumatic Brain Injury
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Zou2019 - 1,520 reference genomes from cultivated human gut bacteria enable functional microbiome analyses.
  • Zupancic2012 - Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome.
  • ...............................
    • GENERAL REFERENCES FOR PHOCAEICOLA MASSILIENSIS
  • Song2010Bergeys - Bacteroides. In Bergey's manual of systematic bacteriology: Vol. 4. The Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes
    • Added: April 08, 2021