Prevotella dentalis

(aka Mitsuokella dentalis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Prevotella dentalis, (aka Mitsuokella dentalis), is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, oval-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. The DNA G+C content is 56-60%. Prevotella dentalis is probably a common, although minor, coloniser of the gut. (Willems1995; Shah2010Bergeys)



  • This organism has been recovered from human faeces and dental disease. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Willems1995); (Shah2010Bergeys);
    Character Response
  • 💧
  • Bile tolerance:
  • Doesn't tolerate 20% bile
  • H+
  • Acid from carbohydrates usually produced:
  • arabinose; D-arabinose; fructose; galactose; glucose; mannose; cellubiose; lactose; maltose; raffinose;
  • Active enzymes:
  • Ala arylamidase; alkaline phosphatase; arabinosidase; N-Ac β-glucosaminidase; α-galactosidase; β-galactosidase; α-glucosidase; Leu-Gly arylamidase;
    • SPECIAL FEATURES (Willems1995); (Shah2010Bergeys);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; succinate;
  • ✗
  • Metabolites not produced:
  • indole;
  • Haemolysis:
  • alpha
  • ✗
  • Nitrate:
  • not reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid;
  • Macrolides:
  • azithromycin; erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Quinolines:
  • ciprofloxacin; levofloxacin;
  • Heterocycles:
  • metronidazole;
  • Miscellaneous antibiotics:
  • clindamycin;

  • N/A

  • Prevotella species are anaerobic Gram_negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Only a few strains have been reported to give rise to opportunistic endogenous infections, including chronic infections, abscesses and anaerobic pneumonia. Emerging studies in humans have linked the increased abundance of Prevotella species at mucosal sites to localized and systemic disease, including periodontitis, bacterial vaginosis, rheumatoid arthritis, metabolic disorders and low_grade systemic inflammation. Intriguingly, Prevotella abundance is reduced within the lung microbiota of patients with asthma and chronic obstructive pulmonary disease. Increased Prevotella abundance is associated with augmented T helper type 17 (Th17) _mediated mucosal inflammation, which is in line with the marked capacity of Prevotella in driving Th17 immune responses in vitro. [PMID: 28542929]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    Prevotella dentalis DSM 3688 (formerly Mitsuokella dentalis), a human oral bacterium often associated with root canal infections was isolated from a human dental root canal. They are singly-occurring Gram-negative, obligately anaerobic, non-spore-forming, nonmotile, blunt-ended, oval rods that measure 0.7 by 1.0 to 2 um. Peritrichous fimbrae and a thick capsulelike structure are present. It is the type strain (adapted from PMID 7547307). In addition to the fully sequenced genome there is another wgs project which is a reference genome for the Human Microbiome Project. [UP000010862]

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Bacteroidia Order:  Bacteroidales Family:  Prevotellaceae Genus:  Prevotella Alt. name:  Mitsuokella dentalis Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Oval-shaped Pigment:  neg
    Health:  Unknown
    Source:  human faeces and dental disease
    DNA G+C(%):  56-60
    Bile reaction(%):  20(neg)
    		Aesculin:  vr Urea:  neg Gelatin:  neg Starch:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  + D-Arabinose:  + Fructose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  neg Xylose:  neg Cellubiose:  + Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  w Sucrose:  w Erythritol:  neg Mannitol:  neg Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Urease:  neg G6PDH6PGDH:  neg α-Arab:  + Ac-β-glcamnd:  + α-Fucosidase:  neg α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  + ArgAA:  neg GluGluAA:  neg GlyAA:  neg HisAA:  neg LeuAA:  neg LeuGlyAA:  + ProAA:  neg PyrrolidAA:  neg PheAA:  neg TyrAA:  neg AlkalineP:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Succinate:  + Indole:  neg Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 0.125, MIC90: 1, RNG: (≤0.015-1)
    penicillin_G:  Var(MIC50): 0.125, MIC90: >32, RNG: (≤0.03->32)
    cefalexin:  Var(MIC50): 2, MIC90: >32, RNG: (0.06->32)
    azithromycin:  S(MIC50): 0.125, MIC90: 1, RNG: (0.06->32)
    erythromycin:  S(MIC50): 0.125, MIC90: 0.5, RNG: (≤0.03->32)
    ciprofloxacin:  S(MIC50): 1, MIC90: 4, RNG: (≤0.5->8)
    levofloxacin:  S(MIC50): 0.5, MIC90: 2, RNG: (≤0.06->8)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  R(MIC50): 8, MIC90: 32, RNG: (0.125->32)
    metronidazole:  S(MIC50): 0.5, MIC90: 1, RNG: (0.06-2)
    clindamycin:  S(MIC50): ≤0.03, MIC90: ≤0.03, RNG: (≤0.03->32)

    References

    SPECIFIC REFERENCES FOR PREVOTELLA DENTALIS
  • Willems1995 - Notes: 16S rRNA Gene Similarities Indicate that Hallella seregens (Moore and Moore) and Mitsuokella dentalis (Haapasalo et al.) Are Genealogically Highly Related and Are Members of the Genus Prevotella: Emended Description of the Genus Prevotella (Shah and Collins) and Description of Prevotella dentalis comb. nov.
  • Shah2010Bergeys - Bergey's manual of systematic bacteriology. Vol. 4, The Bacteroidetes. Family Prevotellaceae, Genus I. Prevotella
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PREVOTELLA DENTALIS
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • King2019 - Baseline human gut microbiota profile in healthy people and standard reporting template.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • Urban2020 - Altered Fecal Microbiome Years after Traumatic Brain Injury
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • Added: February 08, 2021