Prevotella denticola

(aka Bacteroides denticola)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Prevotella denticola, (aka Bacteroides denticola), is a Gram-negative, non-spore-forming, anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 11 gut microbiome compilation studies or metastudies. The DNA G+C content is 49-51%. Prevotella denticola is probably a common, although minor, coloniser of the gut. (Shah1990; Wu1992; Shah2010Bergeys; Shah1981; Watabe1983a)



  • This organism has been recovered from oral cavity and human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread (notes: human and animal pathogen). It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Shah1990); (Wu1992); (Shah2010Bergeys); (Shah1981); (Watabe1983a);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; milk; starch;
  • 💧
  • Bile tolerance:
  • Doesn't tolerate 20% bile
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; glycogen; inulin; starch; lactose; maltose; raffinose; sucrose;
  • ±
  • Strain-dependent acid from carbs:
  • ribose; aesculin;
  • Active enzymes:
  • Ala arylamidase; alkaline phosphatase; N-Ac β-glucosaminidase; fucosidase; α-galactosidase; β-galactosidase; α-glucosidase; Leu-Gly arylamidase;
    • SPECIAL FEATURES (Shah1990); (Wu1992); (Shah2010Bergeys); (Shah1981); (Watabe1983a);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; lactate (trace); isobutyrate (trace); succinate (major); isovalerate (trace); indole (variable);
  • ✗
  • Metabolites not produced:
  • H₂;
  • Haemolysis:
  • beta(absent)
  • ✗
  • Nitrate:
  • not reduced
  • ±
  • Pigments:
  • dark brown (variable)
    • RESPONSE TO ANTIBIOTICS (Wu1992); (Goldstein2018); (Goldstein2013); (Citron2012a); (Citron2011); (Goldstein2006); (Goldstein2000); (Goldstein1999a); (Goldstein1999b); (Shah1981);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin-sulbactam; doripenem; ertapenem; imipenem; meropenem; piperacillin-tazobactam;
  • Cephalosporins:
  • cefalexin; cefotaxime; cefoxitin;
  • ceftazidime;
  • Macrolides:
  • azithromycin; clarithromycin; erythromycin; telithromycin;
  • fidaxomicin;
  • Tetracyclines:
  • minocycline; tetracycline; tigecycline;
  • Quinolines:
  • ciprofloxacin; gatifloxacin; gemifloxacin; levofloxacin; moxifloxacin; trovafloxacin;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; telithromycin;

  • N/A

  • Prevotella denticola is isolated from the human mouth, where it is suspected to cause disease. [UP000008137]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    Cells are nonmotile, non-spore-form- ing, gram-negative, blunt-ended, oval rods that are 0.7 by 1 to 2 p,m. They occur singly. Peritrichous fimbrae and a thick capsulelike structure are present. Obligately anaerobic. Poor growth occurs in liquid media; better growth occurs on blood agar media supplemented with hemolyzed blood. No growth occurs on kanamycin-vancomycin laked blood agar. [PMID: 7547307] Prevotella denticola is isolated from the human mouth, where it is suspected to cause disease. This strain has been sequenced as part of the Human Microbiome Project (HMP). [UP000008137]

  • Bik, E. M., Eckburg, P. B., Gill, S. R., Nelson, K. E., Purdom, E. A., Francois, F., Perez-Perez, G., Blaser, M. J., & Relman, D. A. (2006). Molecular analysis of the bacterial microbiota in the human stomach. Proceedings of the National Academy of Sciences of the United States of America, 103(3), 732–737.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Bacteroidia Order:  Bacteroidales Family:  Prevotellaceae Genus:  Prevotella Alt. name:  Bacteroides denticola Gram stain:  neg O2 Relation.:  anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod Pigment:  dark brown (variable)
    Health:  Unknown
    Source:  oral cavity and human faeces
    DNA G+C(%):  49-51
    Bile reaction(%):  20(neg)
    		Aesculin:  + Urea:  neg Gelatin:  vr Starch:  + Milk:  + Meat:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  d Sorbose:  neg Xylose:  neg Cellubiose:  neg Lactose:  + Maltose:  + Melezitose:  neg Melibiose:  neg Sucrose:  + Trehalose:  neg Amygdalin:  neg Aesculin:  d Glycogen:  + Inulin:  + Starch:  + Pectin:  neg Xylan:  neg Erythritol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg G6PDH6PGDH:  neg Ac-β-glcamnd:  + α-Fucosidase:  + α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  neg GluDC:  neg GlyAP:  neg AlanineAA:  + GluGluAA:  vr GlyAA:  neg LeuAA:  neg LeuGlyAA:  + PyrrolidAA:  neg AlkalineP:  + Lecithinase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Lactate:  trace(+) Isobutyrate:  trace(+) Succinate:  Major(+) Isovalerate:  trace(+) H2:  neg Indole:  vr Pigment:  dark brown (variable)
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (≤0.015-1)
    ampicillin:  Var(MIC50): 1, MIC90: 32, RNG: (0.06–64)
    amp-sulb:  S(MIC50): 0.25, MIC90: 2, RNG: (0.12-4)
    penicillin_G:  Var(MIC50): 0.5, MIC90: 8, RNG: (≤0.03-8)
    piper-taz:  S(MIC50): 0.015, MIC90: 0.06, RNG: (0.015–1)
    doripenem:  S(MIC50): 0.03, MIC90: 0.125, RNG: (≤0.016-0.25)
    ertapenem:  S(MIC50): 0.125, MIC90: 0.5, RNG: (0.03-0.5)
    imipenem:  S(MIC50): 0.03, MIC90: 0.06, RNG: (≤0.016-0.125)
    meropenem:  S(MIC50): 0.06, MIC90: 0.25, RNG: (≤0.016-0.25)
    cefalexin:  S(MIC50): 1, MIC90: 4, RNG: (0.25-32)
    cefotaxime:  S(MIC50): 2, MIC90: 4, RNG: (0.03-16)
    cefoxitin:  S(MIC50): 1, MIC90: 4, RNG: (1-8)
    ceftazidime:  R(MIC50): 32, MIC90: >128, RNG: (1->128)
    azithromycin:  S(MIC50): 0.06, MIC90: 1, RNG: (≤0.03-8)
    erythromycin:  S(MIC50): 0.125, MIC90: 1, RNG: (≤0.03-16)
    fidaxomicin:  R(MIC50): >32, MIC90: >32, RNG: (>32)
    clarithromycin:  S(MIC50): 0.125, MIC90: 4, RNG: (0.03-4)
    roxithromycin:  Var(MIC50): 0.5, MIC90: 8, RNG: (0.125-16)
    telithromycin:  S(MIC50): 0.25, MIC90: 2, RNG: (0.06-16)
    ciprofloxacin:  S(MIC50): 1, MIC90: 2, RNG: (≤0.5-4)
    gatifloxacin:  S(MIC50): 0.25, MIC90: 2, RNG: (0.125-4)
    gemifloxacin:  S(MIC50): 1, MIC90: 2, RNG: (0.03-8)
    levofloxacin:  S(MIC50): 0.5, MIC90: 1, RNG: (0.125-4)
    moxifloxacin:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.032-4)
    ofloxacin:  Var(MIC50): 1, MIC90: 8, RNG: (1-32)
    sparfloxacin:  Var(MIC50): 2, MIC90: 8, RNG: (1–8)
    trovafloxacin:  S(MIC50): 1, MIC90: 4, RNG: (0.06-8)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  Var(MIC50): 0.25, MIC90: 8, RNG: (0.032-16)
    minocycline:  S(MIC50): 0.03, MIC90: 4, RNG: (0.03–8)
    tetracycline:  S(MIC50): 0.25, MIC90: 16, RNG: (0.06–16)
    tigecycline:  S(MIC50): 0.25, MIC90: 0.25, RNG: (0.03–1)
    vancomycin:  R(MIC50): >64, MIC90: >64, RNG: (>64)
    chloramphenicol:  S(12)
    metronidazole:  S(MIC50): 0.25, MIC90: 1, RNG: (≤0.03-2)
    clindamycin:  S(MIC50): ≤0.03, MIC90: 0.25, RNG: (≤0.03->32)

    References

    SPECIFIC REFERENCES FOR PREVOTELLA DENTICOLA
  • Shah1990 - Notes: Prevotella, a New Genus To Include Bacteroides melaninogenicus and Related Species Formerly Classified in the Genus Bacteroides.
  • Wu1992 - Emended Descriptions of Prevotella denticola, Prevotella loescheii, Prevotella veroralis, and Prevotella melaninogenica.
  • Shah2010Bergeys - Bergey's manual of systematic bacteriology. Vol. 4, The Bacteroidetes. Family Prevotellaceae, Genus I. Prevotella
  • Kishikawa2020 - Metagenome-wide association study of gut microbiome revealed novel aetiology of rheumatoid arthritis in the Japanese population
  • Goldstein2018 - Comparative In Vitro Activity of Omadacycline against Dog and Cat Bite Wound Isolates.
  • Goldstein2013 - In vitro activity of Biapenem plus RPX7009, a carbapenem combined with a serine β-lactamase inhibitor, against anaerobic bacteria.
  • Citron2012a - Comparative in vitro activities of LFF571 against Clostridium difficile and 630 other intestinal strains of aerobic and anaerobic bacteria.
  • Citron2011 - In vitro activity of ceftazidime-NXL104 against 396 strains of beta-lactamase-producing anaerobes.
  • Goldstein2006 - In vitro activity of moxifloxacin against 923 anaerobes isolated from human intra-abdominal infections.
  • Goldstein2000 - Comparative in vitro activities of GAR-936 against aerobic and anaerobic animal and human bite wound pathogens.
  • Goldstein1999a - Activity of gatifloxacin compared to those of five other quinolones versus aerobic and anaerobic isolates from skin and soft tissue samples of human and animal bite wound infections.
  • Goldstein1999b - Activities of telithromycin (HMR 3647, RU 66647) compared to those of erythromycin, azithromycin, clarithromycin, roxithromycin, and other antimicrobial agents against unusual anaerobes.
  • Shah1981 - Bacteroides buccalis, sp. nov., Bacteroides denticola, sp. nov., and Bacteroides pentosaceus, sp. nov., new species of the genus Bacteroides from the oral cavity.
  • Watabe1983a - Taxonomic Study of Bacteroides oralis and Related Organisms and Proposal of Bacteroides veroralis sp. nov.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PREVOTELLA DENTICOLA
  • Almeida2019 - A new genomic blueprint of the human gut microbiota.
  • Bik2006 - Molecular analysis of the bacterial microbiota in the human stomach.
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Chung2016 - Modulation of the human gut microbiota by dietary fibres occurs at the species level.
  • King2019 - Baseline human gut microbiota profile in healthy people and standard reporting template.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
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    • Added: February 08, 2021