Prevotella phocaeensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Prevotella phocaeensis is a Gram-negative, non-spore-forming, strictly anaerobic, non-motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 44.6%. Prevotella phocaeensis is probably a rare gut coloniser. (Afouda2017a)



  • This organism has been recovered from human faeces (Afouda2017a). Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Afouda2017a);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 0.5% salt;
  • 💧
  • Bile tolerance:
  • Doesn't tolerate 20% bile
  • pH
  • Acidity tolerance:
  • tolerates pH 6.0-8.0; tolerates pH 8.5;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 28℃; grows at 37℃; doesn't grow at 42℃;
  • H+
  • Acid from carbohydrates usually produced:
  • L-arabinose; ribose; D-tagatose; L-xylose; aesculin; D-lyxose; raffinose; sucrose; adonitol; 2-ketogluconate; 5-ketogluconate; salicin;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; N-Ac β-glucosaminidase; β-galactosidase; β-glucosidase; β-glucuronidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Afouda2017a);
    Character Response
  • ✓
  • Haemolysis:
  • present
    • RESPONSE TO ANTIBIOTICS (Afouda2017a);
    Class Active Resistant
  • Penicillins:
  • imipenem; oxacillin; penicillin G;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • doxycycline;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • metronidazole; trimethoprim-sulfamethoxazole;
  • Vancomycins:
  • teicoplanin;
  • Miscellaneous antibiotics:
  • clindamycin; colistin;

  • Prevotella species at mucosal sites to localized and systemic disease, including periodontitis, bacterial vaginosis, rheumatoid arthritis, metabolic disorders and low_grade systemic inflammation. Intriguingly, Prevotella abundance is reduced within the lung microbiota of patients with asthma and chronic obstructive pulmonary disease. Increased Prevotella abundance is associated with augmented T helper type 17 (Th17) _mediated mucosal inflammation, which is in line with the marked capacity of Prevotella in driving Th17 immune responses in vitro. Studies indicate that Prevotella predominantly activate Toll_like receptor 2, leading to production of Th17_polarizing cytokines by antigen_presenting cells, including interleukin_23 (IL_23) and IL_1. Furthermore, Prevotella stimulate epithelial cells to produce IL_8, IL_6 and CCL20, which can promote mucosal Th17 immune responses and neutrophil recruitment. Prevotella_mediated mucosal inflammation leads to systemic dissemination of inflammatory mediators, bacteria and bacterial products, which in turn may affect systemic disease outcomes. [PMID: 28542929]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    Prevotella species are anaerobic Gram_negative bacteria of the Bacteroidetes phylum, which also includes the clinically important genera Bacteroides and Porphyromonas. Prevotella strains are classically considered commensal bacteria due to their extensive presence in the healthy human body and their rare involvement in infections. Only a few strains have been reported to give rise to opportunistic endogenous infections, including chronic infections, abscesses and anaerobic pneumonia. [PMID: 28542929]

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Bacteroidetes Class:  Bacteroidia Order:  Bacteroidales Family:  Prevotellaceae Genus:  Prevotella Gram stain:  neg O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human faeces (Afouda2017a)
    DNA G+C(%):  44.6
    Lower T(℃):  28(neg)
    Mid T(℃):  37(+)
    High T(℃):  42(neg)
    NaCl 0.5-2%:  0.5(+)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  8.5(+)
    Bile reaction(%):  20(neg)
    		Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  + Fructose:  neg Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg D-Lyxose:  + Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  + Xylose:  neg L-Xylose:  + Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  + Trehalose:  neg Turanose:  neg Amygdalin:  neg Aesculin:  + Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  + D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  + 5-Ketogluconate:  + Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg NAc-α-GA:  neg Salicin:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Glucose:  neg Mannose:  neg Maltose:  neg N_Acetyl_glucosamine:  neg Gluconate:  neg Mannitol:  neg Trp:  neg Adipate:  neg Caprate:  neg Citrate:  neg Malate:  neg Phenylacetate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Ac-β-glcamnd:  + α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  neg β-Glucosidase:  + β-Glucuronidase:  + α-Mannosidase:  neg Chymotrypsin:  neg Trypsin:  neg LeuAA:  neg ValAA:  neg AlkalineP:  + AcidP:  + Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    oxacillin:  S(5; disc)
    penicillin_G:  S(10; disc)
    imipenem:  S(10; disc)
    erythromycin:  S(15; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(30; disc)
    teicoplanin:  S(30; disc)
    rifampicin:  S(30; disc)
    metronidazole:  S(4; disc)
    SXT:  S(25; disc)
    clindamycin:  S(15; disc)
    colistin:  S(50; disc)

    References

    SPECIFIC REFERENCES FOR PREVOTELLA PHOCAEENSIS
  • Afouda2017a - Non-contiguous finished genome sequence and description of Prevotella phocaeensis sp. nov., a new anaerobic species isolated from human gut infected by Clostridium difficile.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PREVOTELLA PHOCAEENSIS
  • Urban2020 - Altered Fecal Microbiome Years after Traumatic Brain Injury
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    • Added: July 12, 2021