Pseudomonas massiliensis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Pseudomonas massiliensis is a Gram-negative, non-spore-forming, aerobic, motile, short rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 62.3%. Pseudomonas massiliensis is unlikely to be a gut coloniser. (Bardet2018)



  • This organism has been recovered from faeces of a healthy volunteer, Brazil. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • GENERAL CHARACTERISTICS (Bardet2018);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 0-2% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 5.3; tolerates pH 8.4; Grows optimally at pH 7.5.
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 45℃; Grows optimally at 37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; aesculin; cellubiose; lactose; maltose; trehalose; glycerol; inositol; mannitol;
  • ⥅
  • Substrates assimilated or utilised:
  • aesculin; gluconate; glucose; maltose; mannitol; mannose; citrate; malate;
  • Active enzymes:
  • alkaline phosphatase; esterase C4; esterase lipase C8; Leu arylamidase; naphthol-ASBI-P;
    • SPECIAL FEATURES (Bardet2018);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • Haemolysis:
  • alpha
  • ✓
  • Nitrate:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Bardet2018);
    Class Active Resistant
  • Penicillins:
  • ertapenem; imipenem; piperacillin-tazobactam;
  • ticarcillin; ticarcillin-clavulanic acid;
  • Cephalosporins:
  • ceftazidime;
  • cefepime;
  • Quinolines:
  • ciprofloxacin; nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; tobramycin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • fosfomycin; trimethoprim-sulfamethoxazole;
  • Miscellaneous antibiotics:
  • colistin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Pseudomonadales Family:  Pseudomonadaceae Genus:  Pseudomonas Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Short Rod
    Health:  Unknown
    Source:  faeces of a healthy volunteer, Brazil
    DNA G+C(%):  62.3
    Opt. T:  37℃
    Lower T(℃):  25(+)
    High T(℃):  45(+)
    NaCl 0.5-2%:  0-2(+)
    Opt. pH:  7.5
    pH 4.2-5.9:  5.3(+)
    pH >8:  8.4(+)
    		Urea:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  + Glucose:  + Cellubiose:  + Lactose:  + Maltose:  + Trehalose:  + Aesculin:  + Glycerol:  + Inositol:  + Mannitol:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  neg Glucose:  + Mannose:  + Maltose:  + Aesculin:  + Gluconate:  + Mannitol:  + Citrate:  + Malate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg Chymotrypsin:  neg Trypsin:  neg CystineAA:  neg LeuAA:  + ValAA:  neg AlkalineP:  + AcidP:  neg Esterase(C4):  + EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    aztreonam:  Var
    piper-taz:  Sens
    ticarcillin:  Res
    tica-clav:  Res
    ertapenem:  Sens
    imipenem:  Sens
    cefepime:  Res
    ceftazidime:  Sens
    gentamicin:  Sens
    tobramycin:  Sens
    ciprofloxacin:  Sens
    nalidixic-acid:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    rifampicin:  Res
    fosfomycin:  Res
    SXT:  Res
    colistin:  Sens

    References

    SPECIFIC REFERENCES FOR PSEUDOMONAS MASSILIENSIS
  • Bardet2018 - Microbial Culturomics Application for Global Health: Noncontiguous Finished Genome Sequence and Description of Pseudomonas massiliensis Strain CB-1T sp. nov. in Brazil.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PSEUDOMONAS MASSILIENSIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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    • Added: January 14, 2022