Pseudomonas stutzeri

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Pseudomonas stutzeri is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 5 gut microbiome compilation studies or metastudies. The DNA G+C content is 60.6-66.3%. Pseudomonas stutzeri is likely to be transient and not a long-term gut coloniser. (Palleroni2005bBergey)



  • This organism has been recovered from soil, clinical sources (sputum, infection, wound - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: opportunistic in immunocompromised patients). It is an opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • GENERAL CHARACTERISTICS (Palleroni2005bBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • starch;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 8.5% salt;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 4℃; strain-variable at 42(d); Grows optimally at 35℃.
  • ⥅
  • Substrates assimilated or utilised:
  • glucose; glycerol; maltose; starch; alanine; D-alanine; glutamate; leucine; proline; acetate; azelate; butyrate; caprate; caprylate; citrate; fumarate; glycolate; 3-hydroxybutyrate; itaconate; lactate; L-malate; mesaconate; pyruvate; sebacate; succinate; glutamine;
  • ±
  • Strain-dependent substrate utilisation:
  • ethanolamine; fructose; gluconate; mannitol; propanol; δ-aminovalerate; aspartate; glycine; isoleucine; phenylalanine; serine; tyrosine; valine; aconitate; adipate; benzoate; caproate; glutarate; glycerate; heptanoate; 4-hydroxybenzoate; isovalerate; malate; malonate; mucate; nonanoate; propionate; saccharate; valerate;
  • Active enzymes:
  • catalase; esterase lipase C8; Leu arylamidase; lipase; oxidase;
    • SPECIAL FEATURES (Palleroni2005bBergey);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Haemolysis:
  • absent
  • ✓
  • Nitrate:
  • reduced
  • ±
  • Pigments:
  • reddish-brown (variable)
    • RESPONSE TO ANTIBIOTICS
    Class Active Resistant
  • Penicillins:
  • imipenem;
  • amoxicillin-clavulanic acid; ertapenem; ticarcillin-clavulanic acid;
  • Cephalosporins:
  • cefaclor; cefalexin; cefamandole; cefixime; cefoxitin; cefpodoxime; cefprozil; cefuroxime; cephalothin;
  • Quinolines:
  • ciprofloxacin;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Pseudomonadales Family:  Pseudomonadaceae Genus:  Pseudomonas Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  reddish-brown (variable)
    Health:  Unknown
    Source:  soil, clinical sources (sputum, infection, wound - CCUG) and human faeces
    DNA G+C(%):  60.6-66.3
    Opt. T:  35℃
    Low T(℃):  4(neg)
    High T(℃):  42(d)
    NaCl >6%:  8.5(neg)
    		Aesculin:  neg Urea:  neg Gelatin:  neg Starch:  + Arginine:  neg Tween:  80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Glucose:  neg Rhamnose:  neg Cellubiose:  neg Maltose:  neg Sucrose:  neg Trehalose:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg 5-Ketogluconate:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg L-Arabinose:  neg Fructose:  d Galactose:  neg Glucose:  + Mannose:  neg Rhamnose:  neg Ribose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  + Sucrose:  neg Trehalose:  neg Adonitol:  neg Erythritol:  neg Ethanolamine:  d Gluconate:  d Glycerol:  + Inositol:  neg Mannitol:  d Propanol:  d Salicin:  neg Sorbitol:  neg Starch:  + Ala:  + D-Ala:  + β-Ala:  neg 4-Aminobutyrate:  neg 5-Aminovalerate:  d Arg:  neg Asp:  d Betaine:  neg L-Citrulline:  neg Glu:  + Gly:  d Hippurate:  neg Histamine:  neg His:  neg Ile:  d DL-Kynurenine:  neg Leu:  + Lys:  neg Norleucine:  neg Orn:  neg Phe:  d Pro:  + Sarcosine:  neg Ser:  d Thr:  neg Trigonelline:  neg Tryptamine:  neg Tyr:  d Val:  d Acetate:  + Aconitate:  d Adipate:  d Azelate:  + Benzoate:  d Butyrate:  + Caprate:  + Caproate:  d Caprylate:  + Citraconate:  neg Citrate:  + Fumarate:  + Glutarate:  d Glycerate:  d Glycolate:  + Heptanoate:  d 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  d Isobutyrate:  neg Isovalerate:  d Itaconate:  + 2-Ketogluconate:  neg Lactate:  + Levulinate:  neg Malate:  d L-Malate:  + Maleate:  neg Malonate:  d L-Mandelate:  neg Mesaconate:  + Mucate:  d Nonanoate:  d Phenol:  neg Phenylacetate:  neg Pimelate:  neg Propionate:  d Pyruvate:  + Saccharate:  d Sebacate:  + Suberate:  neg Succinate:  + D-Tartrate:  neg L-Tartrate:  neg Valerate:  d
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg LysDC:  neg OrnDC:  neg LeuAA:  + AcidP:  neg Esterase(C4):  vr EstLip(C8):  + Lecithinase:  neg Lipase:  + Lipase(C14):  vr
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg Pigment:  reddish-brown (variable)
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  R(MIC50): >32, MIC90: >32, RNG: (1->32)
    carbenicil:  Var(MIC50): 2, MIC90: 64
    tica-clav:  R(MIC50): 8, MIC90: 64, RNG: (0.25->64)
    ertapenem:  R(MIC50): >8, MIC90: >16, RNG: (≤0.008->16)
    imipenem:  S(MIC50): 0.5, MIC90: 0.5, RNG: (0.06-1)
    cefaclor:  R(>64/>64)
    cefalexin:  R(32/-)
    cefamandole:  R(>64/-)
    cefepime:  Var(MIC50): 0.25, MIC90: >64, RNG: (≤0.06->64)
    cefixime:  R(MIC50): >64, MIC90: >64, RNG: (8->64)
    cefoperazone:  Var(MIC50): 1, MIC90: 8
    cefotaxime:  Var(MIC50): 1, MIC90: 8
    cefoxitin:  R(16/64)
    cefpodoxime:  R(MIC50): >64, MIC90: >64, RNG: (8->64)
    cefprozil:  R(>64/>64)
    ceftazidime:  Var(MIC50): 1, MIC90: >64, RNG: (≤0.06->64)
    cefuroxime:  R(MIC50): >64, MIC90: >64, RNG: (8-64)
    cephalothin:  R(>64/>64)
    moxalactam:  Var(MIC50): 2, MIC90: 4, RNG: (0.5-8)
    gentamicin:  Var(MIC50): 0.5, MIC90: >64
    ciprofloxacin:  S(≤0.06/≤0.06)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)

    References

    SPECIFIC REFERENCES FOR PSEUDOMONAS STUTZERI
  • Palleroni2005bBergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Pseudomonadaceae, Genus I. Pseudomonas
  • Cassir2015 - Clostridium butyricum Strains and Dysbiosis Linked to Necrotizing Enterocolitis in Preterm Neonates
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PSEUDOMONAS STUTZERI
  • Cassir2015 - Clostridium butyricum Strains and Dysbiosis Linked to Necrotizing Enterocolitis in Preterm Neonates
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
  • ...............................
    • GENERAL REFERENCES FOR PSEUDOMONAS STUTZERI
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: April 08, 2021