Pseudomonas tolaasii

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Pseudomonas tolaasii is a Gram-negative, non-spore-forming, aerobic, motile, rod-shaped bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 61%. Pseudomonas tolaasii is likely to be transient and not a long-term gut coloniser. (Cutri1984; Lelliott1966; Paine1919; Stanier1966; Palleroni2005bBergey)



  • This organism has been recovered from mushroom disease and has been detected in human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread (notes: plant pathogen). Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Borderline cold mesophile, so unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Cutri1984); (Lelliott1966); (Paine1919); (Stanier1966); (Palleroni2005bBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • casein; gelatin; tyrosine;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • milk;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 6℃; Grows optimally at 25℃.
  • H+
  • Acid from carbohydrates usually produced:
  • sucrose;
  • ⥅
  • Substrates assimilated or utilised:
  • glucose; inositol; sorbitol; trehalose; β-alanine; arginine; valine; 2-ketogluconate;
  • ±
  • Strain-dependent substrate utilisation:
  • adonitol; L-arabinose; ethanol;
  • Active enzymes:
  • arginine dihydrolase; catalase; lipase; oxidase;
    • SPECIAL FEATURES (Paine1919); (Stanier1966); (Palleroni2005bBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • indole (weak);
  • ✓
  • Nitrate:
  • reduced
  • Pigments:
  • fluorescent

  • Pseudomonas tolaasii is a spoilage pathogen of mushroom crops. [PMID: 24946855]

  • GutFeeling KnowledgeBase COMMENTS [Website]

    Pseudomonas tolaasii is a Gram-negative, naturally soil-dwelling bacterial pathogen that causes brown blotch disease in several varieties of cultivated mushrooms [1-3]. The disease is characterised by brown lesions on the outer layers (2-3 mm depth) of the mushroom pileus and stipe, which range from small, light brown spots to larger, dark, sunken and wet lesions, depending on disease severity. This brown discolouration results from mushroom production of melanin, which is a defence response induced in this case by P. tolaasii producing the toxin tolaasin. Tolaasin is an 18-amino acid lipodepsipeptidide that forms ion channels and also acts as a biosurfactant to disrupt the plasma membrane of mushroom cells, allowing P. tolaasii access to cell-nutrients. [PMID: 24946855]

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Pseudomonadales Family:  Pseudomonadaceae Genus:  Pseudomonas Gram stain:  neg O2 Relation.:  aerobic Spore:  No spore Motility:  Swimming Morphology:  Rod Pigment:  fluorescent
    Health:  Unknown
    Source:  mushroom disease and has been detected in human faeces
    DNA G+C(%):  61
    Opt. T:  25℃
    Low T(℃):  6(neg)
    		Aesculin:  neg Gelatin:  + Starch:  neg Casein:  + Tyrosine:  + Milk:  curdle
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Sucrose:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  d Glucose:  + Sucrose:  neg Trehalose:  + Adonitol:  d Ethanol:  d Inositol:  + Sorbitol:  + β-Ala:  + Arg:  + Val:  + 2-Ketogluconate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + ArgDH:  + Lipase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  w Pigment:  fluorescent

    References

    SPECIFIC REFERENCES FOR PSEUDOMONAS TOLAASII
  • Cutri1984 - Characteristics of pathogenic non-fluorescent (smooth) and non-pathogenic fluorescent (rough) forms of Pseudomonas tolaasii and Pseudomonas gingeri.
  • Lelliott1966 - A Determinative Scheme for the Fluorescent Plant Pathogenic Pseudomonads.
  • Paine1919 - Studies In Bacteriosis II.: A Brown Blotch Disease of Cultivated Mushrooms.
  • Stanier1966 - The Aerobic Pseudomonads a Taxonomic Study.
  • Palleroni2005bBergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Pseudomonadaceae, Genus I. Pseudomonas
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR PSEUDOMONAS TOLAASII
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • King2019 - Baseline human gut microbiota profile in healthy people and standard reporting template.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • ...............................
    • Added: February 08, 2021