Romboutsia lituseburensis

(aka Clostridium lituseburense)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Romboutsia lituseburensis, (aka Clostridium lituseburense), is a Gram-positive, spore-forming, anaerobic, motile, rod-shaped bacterium. It has been detected in at least 7 gut microbiome compilation studies or metastudies. The DNA G+C content is 27%. Romboutsia lituseburensis is probably a common, although minor, coloniser of the gut. (Gerritsen2014; Rainey2011fBergey)



  • This organism has been recovered from human faeces and soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Gerritsen2014); (Rainey2011fBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • gelatin; meat;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • milk;
  • 💧
  • Bile tolerance:
  • Doesn't tolerate 20% bile
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; maltose; sucrose;
  • ±
  • Strain-dependent acid from carbs:
  • ribose;
  • Active enzymes:
  • lecithinase;
    • SPECIAL FEATURES (Gerritsen2014); (Rainey2011fBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • formate; acetate; propionate (minor); butyrate (minor); lactate (minor); isovalerate (minor); Hâ‚‚ (minor);
  • ✗
  • Metabolites not produced:
  • indole;

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Peptostreptococcaceae Genus:  Romboutsia Alt. name:  Clostridium lituseburense Gram stain:  + O2 Relation.:  anaerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and soil
    DNA G+C(%):  27
    Bile reaction(%):  20(neg)
    		Aesculin:  neg Gelatin:  + Starch:  neg Milk:  d Meat:  +
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Fucose:  neg D-Fucose:  neg Galactose:  neg Glucose:  + Mannose:  neg D-Lyxose:  neg Rhamnose:  neg Ribose:  d Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Sucrose:  + Trehalose:  neg Turanose:  neg Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Gluconate:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Me-α-D-Glc:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg Lecithinase:  + Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  + Acetate:  + Propionate:  minor(+) Butyrate:  minor(+) Lactate:  minor(+) Isovalerate:  minor(+) H2:  minor(+) Indole:  neg

    References

    SPECIFIC REFERENCES FOR ROMBOUTSIA LITUSEBURENSIS
  • Gerritsen2014 - Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat, and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.
  • DePalma2010 - Intestinal dysbiosis and reduced immunoglobulin-coated bacteria associated with coeliac disease in children
  • Kerckhoffs2009 - Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients
  • Takahashi2016 - Reduced Abundance of Butyrate-Producing Bacteria Species in the Fecal Microbial Community in Crohn's Disease
  • Luna2016 - Distinct Microbiome-Neuroimmune Signatures Correlate With Functional Abdominal Pain in Children With Autism Spectrum Disorder.
  • Rainey2011fBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Clostridiaceae, Genus I. Clostridium - Cluster XI
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ROMBOUTSIA LITUSEBURENSIS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • ...............................
    • GENERAL REFERENCES FOR ROMBOUTSIA LITUSEBURENSIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021