Roseburia faecis

(aka Agathobacter faecis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Roseburia faecis, (aka Agathobacter faecis), is a Gram-variable, strictly anaerobic, motile, rod-shaped - curved bacterium. It has been detected in at least 16 gut microbiome compilation studies or metastudies. The DNA G+C content is 42.0%. Roseburia faecis is often a widespread coloniser of gut. (Duncan2006)



  • This organism has been recovered from human faeces. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal. Robust growth can have positive consequences for gut health.
    • QUIRKS
  • In 99% of Europeans (unseenbio.com).
    • GENERAL CHARACTERISTICS (Duncan2006);
    Character Response
  • 🌡
  • Temperature tolerance:
  • Grows optimally at 37℃.
  • ⥅
  • Substrates assimilated or utilised:
  • cellubiose; fructose; glucose; maltose; melibiose; pectin; raffinose; sorbitol; starch; xylose; acetate;
  • Active enzymes:
  • α-galactosidase; β-galactosidase; α-glucosidase;
  • ±
  • Strain-dependent active enzymes:
  • β-glucosidase;
    • SPECIAL FEATURES (Duncan2006);
    Character Response
  • ✓
  • Metabolites produced:
  • formate; butyrate (major); lactate (minor);
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • Nitrate:
  • not reduced
  • NOTES

    (fae′cis. L. gen. n. referring to faecal origin).

    Gram-variable, motile, slightly curved rods. Cells measure approximately 0.5×1.5–5 μm. Optimum growth temperature is 37 °C. Strictly anaerobic. Good growth occurs on M2GSC agar at 37 °C and after incubation for 48 h forms creamy white translucent colonies with entire edges, approximately 1–3 mm in diameter. Chemo-organotrophic. Utilizes fructose, glucose, maltose, cellobiose, raffinose, xylose, sorbitol, melibiose and amylopectin starch as energy sources for growth. Weak growth occurs with inulin or oat spelt xylan as energy source. Arabinose, sucrose and glycerol are not utilized for growth. Butyrate and formate are major products and lactate a minor product from glucose (0.2 %), with net consumption of acetate present in the medium. Catalase-negative. The G+C content of the DNA of the type strain is 42.0 mol%.

    Isolated from human faeces in Aberdeen, Scotland. The type strain is M72/1T (=DSM 16840T=NCIMB 14031T).

  • Duncan, S. H., Aminov, R. I., Scott, K. P., Louis, P., Stanton, T. B., & Flint, H. J. (2006). Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces. International Journal of Systematic and Evolutionary Microbiology, 56(Pt 10), 2437–2441.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Lachnospiraceae Genus:  Roseburia Alt. name:  Agathobacter faecis Gram stain:  vr O2 Relation.:  strictly anaerobic Motility:  Swimming Morphology:  Rod - curved
    Health:   Positive
    Source:  human faeces
    DNA G+C(%):  42.0
    Opt. T:  37℃
    		Urea:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Mannose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg Fructose:  + Glucose:  + Xylose:  + Cellubiose:  + Maltose:  + Melibiose:  + Raffinose:  + Sucrose:  neg Glycerol:  neg Inulin:  w Pectin:  + Sorbitol:  + Starch:  + Xylan:  w Acetate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  d β-Glucuronidase:  neg ArgDH:  neg GluDC:  neg AlanineAA:  neg GluGluAA:  neg GlyAA:  neg LeuAA:  neg LeuGlyAA:  neg PyrrolidAA:  neg AlkalineP:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    Fibre, Resistant Starch, Acetate, Glucose, Galactose, Mannitol

    None/Unknown

    Branched-Chain AA, Cobalamin, Folate, Biotin, Riboflavin, Acetate, Lactate

    None/Unknown

    		 Formate:  + Butyrate:  Major(+) Lactate:  minor(+) Indole:  neg

    References

    SPECIFIC REFERENCES FOR ROSEBURIA FAECIS
  • Duncan2006 - Proposal of Roseburia faecis sp. nov., Roseburia hominis sp. nov. and Roseburia inulinivorans sp. nov., based on isolates from human faeces.
  • Borgo2017 - Microbiota in anorexia nervosa: The triangle between bacterial species, metabolites and psychological tests
  • Breban2017 - Faecal microbiota study reveals specific dysbiosis in spondyloarthritis
  • DeAngelis2013 - Fecal microbiota and metabolome of children with autism and pervasive developmental disorder not otherwise specified
  • Gargari2018 - Evidence of dysbiosis in the intestinal microbial ecosystem of children and adolescents with primary hyperlipidemia and the potential role of regular hazelnut intake
  • Gryp2020 - Isolation and Quantification of Uremic Toxin Precursor-Generating Gut Bacteria in Chronic Kidney Disease Patients
  • Hedin2015 - Siblings of patients with Crohn's disease exhibit a biologically relevant dysbiosis in mucosal microbial metacommunities
  • Huang2019 - Analysis of microbiota in elderly patients with Acute Cerebral Infarction
  • Jackson2016 - Signatures of early frailty in the gut microbiota
  • KowalskaDuplaga2019 - Differences in the intestinal microbiome of healthy children and patients with newly diagnosed Crohn's disease
  • Panelli2020 - Comparative Study of Salivary, Duodenal, and Fecal Microbiota Composition Across Adult Celiac Disease
  • Santiago2016 - Alteration of the serum microbiome composition in cirrhotic patients with ascites
  • Shen2018 - Analysis of gut microbiota diversity and auxiliary diagnosis as a biomarker in patients with schizophrenia: A cross-sectional study
  • Zheng2020a - Specific gut microbiome signature predicts the early-stage lung cancer
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ROSEBURIA FAECIS
  • Browne2016 - Culturing of 'unculturable' human microbiota reveals novel taxa and extensive sporulation.
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Jeong2021 - The effect of taxonomic classification by full-length 16S rRNA sequencing with a synthetic long-read technology
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Li2019b - Disordered intestinal microbes are associated with the activity of Systemic Lupus Erythematosus
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • Minerbi2019 - Altered microbiome composition in individuals with fibromyalgia
  • PerezBrocal2015 - Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects Potential Interactions and Biomarkers
  • PerisBondia2011 - The active human gut microbiota differs from the total microbiota.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Walker2011 - High-throughput clone library analysis of the mucosa-associated microbiota reveals dysbiosis and differences between inflamed and non-inflamed regions of the intestine in inflammatory bowel disease.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • Zou2019 - 1,520 reference genomes from cultivated human gut bacteria enable functional microbiome analyses.
  • Zupancic2012 - Analysis of the Gut Microbiota in the Old Order Amish and Its Relation to the Metabolic Syndrome.
  • deGoffau2013 - Fecal microbiota composition differs between children with β-cell autoimmunity and those without.
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    • GENERAL REFERENCES FOR ROSEBURIA FAECIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 05, 2021