Rothia dentocariosa

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Rothia dentocariosa is a Gram-positive, non-spore-forming, aerobic, non-motile, filamentous bacterium. It has been detected in at least 13 gut microbiome compilation studies or metastudies. Rothia dentocariosa is probably a common, although minor, coloniser of the gut. (Daneshvar2004)



  • This organism has been recovered from clinical sources (pleural fluid, infection, blood, sputum, bucca - CCUG) and human faeces. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Daneshvar2004);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin;
  • ±
  • Strain-dependent hydrolysis or digestion:
  • gelatin;
  • 🧂
  • Salt tolerance:
  • doesn't tolerate 6% salt;
  • 🌡
  • Temperature tolerance:
  • grows weakly at 25℃; grows at 35℃; doesn't grow at 42℃;
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; maltose; sucrose; trehalose; glycerol; α-methyl glucoside;
  • Active enzymes:
  • Ala arylamidase; Ala-Phe-Pro arylamidase; α-glucosidase; Gly arylamidase; Leu arylamidase; Leu-Gly arylamidase; pyrrolidine arylamidase;
    • SPECIAL FEATURES (Daneshvar2004);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; lactate (major); succinate (minor); H₂S (most strains);
  • ✗
  • Metabolites not produced:
  • indole;
  • VP test:
  • activity is variable
  • ✗
  • Haemolysis:
  • absent
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • ✓
  • Pigments:
  • produced
    • RESPONSE TO ANTIBIOTICS (Goldstein2000);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; penicillin G;
  • Macrolides:
  • azithromycin; erythromycin;
  • Tetracyclines:
  • minocycline; tetracycline;
  • Quinolines:
  • levofloxacin; moxifloxacin;
  • Vancomycins:
  • vancomycin;

  • Taylor, G. R., Kropp, K. D., & Molina, T. C. (1985). Nine-year microflora study of an isolator-maintained immunodeficient child. Applied and Environmental Microbiology, 50(6), 1349–1356.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Micrococcales Family:  Micrococcaceae Genus:  Rothia Gram stain:  + O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Filamentous Pigment:  +
    Health:  Unknown
    Source:  clinical sources (pleural fluid, infection, blood, sputum, bucca - CCUG) and human faeces
    Lower T(℃):  25(w)
    Mid T(℃):  35(+)
    High T(℃):  42(neg)
    NaCl >6%:  6(neg)
    		Aesculin:  + Urea:  neg Gelatin:  d Starch:  neg Casein:  neg Hippurate:  neg Milk:  neg Tween:  40(neg) 80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg L-Arabinose:  neg Fructose:  + Glucose:  + Mannose:  + Ribose:  neg D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  + Melezitose:  neg Sucrose:  + Trehalose:  + Turanose:  vr Dextrin:  neg Glycogen:  neg Inulin:  neg Starch:  neg D-Arabitol:  neg Glycerol:  + Mannitol:  neg Sorbitol:  neg Xylitol:  neg Me-α-D-Glc:  + NAc-α-GA:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Acetate:  neg Lactate:  neg Pyruvate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  vr Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  + β-Glucosidase:  vr β-Glucuronidase:  neg α-Mannosidase:  neg β-Mannosidase:  neg ArgDH:  neg GluDC:  neg LysDC:  neg OrnDC:  neg AlanineAA:  + AlaPheProAA:  + GluGluAA:  neg GlyAA:  + LeuAA:  + LeuGlyAA:  + PyrrolidAA:  + AlkalineP:  neg AcidP:  neg Esterase(C4):  vr EstLip(C8):  vr Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Lactate:  Major(+) Succinate:  minor(+) H2S:  d(+) Indole:  neg Pigment:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(MIC50): 0.25, MIC90: 1, RNG: (0.016-64)
    penicillin:  Var(MIC50): 1, MIC90: >32, RNG: (0.004-32)
    penicillin_G:  S(MIC50): ≤0.015, MIC90: -, RNG: (≤0.015–1)
    azithromycin:  S(MIC50): 0.125, MIC90: -, RNG: (≤0.015–4)
    erythromycin:  S(MIC50): 0.06, MIC90: -, RNG: (≤0.015–1)
    levofloxacin:  S(MIC50): 0.25, MIC90: 2, RNG: (0.006-2)
    moxifloxacin:  S(MIC50): 0.125, MIC90: 0.25, RNG: (0.016-0.5)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  Var(MIC50): 1, MIC90: 8, RNG: (0.064->32)
    minocycline:  S(MIC50): 0.06, MIC90: -, RNG: (≤0.015–0.125)
    tetracycline:  S(MIC50): 0.125, MIC90: -, RNG: (0.03–4)
    vancomycin:  S(MIC50): 0.25, MIC90: -, RNG: (≤0.015–>8)
    clindamycin:  Var(MIC50): 2, MIC90: >256, RNG: (0.016->256)

    References

    SPECIFIC REFERENCES FOR ROTHIA DENTOCARIOSA
  • Daneshvar2004 - Identification of Some Charcoal-Black-Pigmented CDC Fermentative Coryneform Group 4 Isolates as Rothia dentocariosa and Some as Corynebacterium aurimucosum : Proposal of Rothia dentocariosa emend. Georg and Brown 1967, Corynebacterium aurimucosum emend. Yassin et al. 2002, and Corynebacterium nigricans Shukla et al. 2003 pro synon. Corynebacterium aurimucosum.
  • Sokol2019 - Intestinal dysbiosis in inflammatory bowel disease associated with primary immunodeficiency
  • Goldstein2000 - Comparative in vitro activities of GAR-936 against aerobic and anaerobic animal and human bite wound pathogens.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR ROTHIA DENTOCARIOSA
  • Bik2006 - Molecular analysis of the bacterial microbiota in the human stomach.
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • De2020 - Metagenomic analysis of gut microbiome and resistome of diarrheal fecal samples from Kolkata, India, reveals the core and variable microbiota including signatures of microbial dark matter.
  • Dubourg2013 - The gut microbiota of a patient with resistant tuberculosis is more comprehensively studied by culturomics than by metagenomics.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Li2019b - Disordered intestinal microbes are associated with the activity of Systemic Lupus Erythematosus
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • PerezBrocal2015 - Metagenomic Analysis of Crohn's Disease Patients Identifies Changes in the Virome and Microbiome Related to Disease Status and Therapy, and Detects Potential Interactions and Biomarkers
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Taylor1985 - Nine-year microflora study of an isolator-maintained immunodeficient child.
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
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    • Added: April 08, 2021