Ruminiclostridium josui

(aka Clostridium josui)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Ruminiclostridium josui, (aka Clostridium josui), is a Gram-positive, spore-forming, strictly anaerobic, non-motile, rod-shaped - curved bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 40%. Ruminiclostridium josui is probably a rare gut coloniser. (Sukhumavasi1988)



  • This organism has been recovered from compost, Thailand. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • QUIRKS
  • Strongly degrades and consumes cellulose.
    • GENERAL CHARACTERISTICS (Sukhumavasi1988);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; cellulose;
  • 💧
  • Bile tolerance:
  • Doesn't tolerate bile
  • pH
  • Acidity tolerance:
  • Grows optimally at pH 7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 60℃; Grows optimally at 45℃.
  • H+
  • Acid from carbohydrates usually produced:
  • cellulose; maltose;
  • ⥅
  • Substrates assimilated or utilised:
  • arabinose; cellubiose; cellulose; glucose; maltose; xylan; xylose;
  • ±
  • Strain-dependent substrate utilisation:
  • lactose; melibiose;
    • SPECIAL FEATURES (Sukhumavasi1988);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate (trace); butyrate (trace); ethanol; COâ‚‚; Hâ‚‚;
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • NO3➔NO2:
  • not reduced

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Clostridia Order:  Eubacteriales Family:  Clostridiaceae Genus:  Ruminiclostridium Alt. name:  Clostridium josui Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  Endospore Motility:  Sessile Morphology:  Rod - curved
    Health:  Unknown
    Source:  compost, Thailand
    DNA G+C(%):  40
    Opt. T:  45℃
    Lower T(℃):  25(+)
    High T(℃):  60(+)
    Opt. pH:  7.0
    Bile reaction(%):  neg
    		Aesculin:  + Gelatin:  neg Starch:  neg Casein:  neg Milk:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Fructose:  neg Galactose:  neg Mannose:  neg Lactose:  w Maltose:  + Melibiose:  w Sucrose:  neg Cellulose:  + Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + Fructose:  neg Galactose:  neg Glucose:  + Rhamnose:  neg Xylose:  + Cellubiose:  + Lactose:  d(neg) Maltose:  + Melezitose:  neg Melibiose:  d(neg) Raffinose:  neg Sucrose:  neg Trehalose:  neg Adonitol:  neg Amygdalin:  neg Cellulose:  + Dextrin:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Inulin:  neg Mannitol:  neg Pectin:  neg Salicin:  neg Sorbitol:  neg Starch:  neg Xylan:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Lecithinase:  neg Lipase:  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Propionate:  trace(+) Butyrate:  trace(+) Ethanol:  + CO2:  + H2:  + Indole:  neg

    References

    SPECIFIC REFERENCES FOR RUMINICLOSTRIDIUM JOSUI
  • Sukhumavasi1988 - Clostridium josui sp. nov., a Cellulolytic, Moderate Thermophilic Species from Thai Compost
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR RUMINICLOSTRIDIUM JOSUI
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • McLaughlin2010 - The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing.
  • ...............................
    • Added: February 09, 2022