General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview


  • Rummeliibacillus pycnus, (aka Bacillus pycnus), is a Gram-positive, spore-forming, strictly aerobic, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 35%. Rummeliibacillus pycnus is likely to be transient and not a long-term gut coloniser. (Vaishampayan2009; Nakamura2002)



  • This organism has been recovered from soil, Japan. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal.

  • QUIRKS
  • Obligate aerobe; unlikely to be a gut coloniser.

  • GENERAL CHARACTERISTICS (Vaishampayan2009); (Nakamura2002);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 5% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 10℃; grows at 45℃; Grows optimally at 28-30℃.
  • H+
  • Acid from carbohydrates usually produced:
  • glucose;
  • Substrates assimilated or utilised:
  • 3-hydroxybutyrate; pyruvate;
  • Active enzymes:
  • catalase;

  • SPECIAL FEATURES (Vaishampayan2009); (Nakamura2002);
    Character Response
  • Metabolites produced:
  • indole;
  • Metabolites not produced:
  • Hâ‚‚S;
  • VP test:
  • not active
  • Lysozyme:
  • inhibited (0.001%)
  • NO3➔NO2:
  • not reduced
  • Pigments:
  • not produced

  • RESPONSE TO ANTIBIOTICS (Nakamura2002);
    Class Active Resistant
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Aminoglycosides:
  • streptomycin; tobramycin;
  • Heterocycles:
  • chloramphenicol;

  • Details


    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Planococcaceae Genus:  Rummeliibacillus Alt. name:  Bacillus pycnus Gram stain:  + O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming Morphology:  Rod Pigment:  neg
    Health:  Unknown
    Source:  soil, Japan
    DNA G+C(%):  35
    Opt. T:  28-30℃
    Low T(℃):  10(+)
    High T(℃):  45(+)
    NaCl 3-5%:  5(+)
    Urea:  neg Gelatin:  neg Starch:  neg Casein:  neg Tyrosine:  neg Tween:  40(neg) 80(neg)

    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg Galactose:  neg Glucose:  + Mannose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Mannitol:  neg

    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Ala:  neg Citrate:  neg 3-Hydroxybutyrate:  + Propionate:  neg Pyruvate:  +

    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  +

    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    H2S:  neg Indole:  + Pigment:  neg

    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    streptomycin:  Sens
    tobramycin:  Sens
    erythromycin:  Sens
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  Sens
    chloramphenicol:  Sens

    References


    SPECIFIC REFERENCES FOR RUMMELIIBACILLUS PYCNUS
  • Vaishampayan2009 - Description of Rummeliibacillus stabekisii gen. nov., sp. nov. and reclassification of Bacillus pycnus Nakamura et al. 2002 as Rummeliibacillus pycnus comb. nov.
  • Nakamura2002 - Bacillus pycnus sp. nov. and Bacillus neidei sp. nov., round-spored bacteria from soil.
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  • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR RUMMELIIBACILLUS PYCNUS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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