Ruoffia tabacinasalis

(aka Facklamia tabacinasalis)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Ruoffia tabacinasalis, (aka Facklamia tabacinasalis), is a Gram-positive, non-spore-forming, facultatively anaerobic, coccus bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 40%. Ruoffia tabacinasalis is probably a rare gut coloniser. (Collins1999a; Pfleiderer2013)



  • This organism has been recovered from human faeces, and tobacco. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Is a known gut commensal.
    • GENERAL CHARACTERISTICS (Collins1999a); (Pfleiderer2013);
    Character Response
  • 🌡
  • Temperature tolerance:
  • grows at 30℃;
  • Active enzymes:
  • α-galactosidase;
    • SPECIAL FEATURES (Collins1999a);
    Character Response
  • ✗
  • VP test:
  • not active
  • Haemolysis:
  • alpha
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS (LaClaire2000);
    Class Active Resistant
  • Penicillins:
  • amoxicillin; meropenem; penicillin;
  • Macrolides:
  • quinupristin-dalfopristin;
  • Quinolines:
  • levofloxacin; trovafloxacin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • chloramphenicol;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;

  • Pfleiderer, A., Lagier, J.-C., Armougom, F., Robert, C., Vialettes, B., & Raoult, D. (2013). Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample. European Journal of Clinical Microbiology & Infectious Diseases: Official Publication of the European Society of Clinical Microbiology, 32(11), 1471–1481.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Lactobacillales Family:  Aerococcaceae Genus:  Ruoffia Alt. name:  Facklamia tabacinasalis Gram stain:  + O2 Relation.:  facultatively anaerobic Spore:  No spore Morphology:  Coccus Pigment:  neg
    Health:  Unknown
    Source:  human faeces, and tobacco
    DNA G+C(%):  40
    Lower T(℃):  30(+)
    		Hippurate:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Maltose:  neg Sucrose:  neg Trehalose:  neg D-Arabitol:  neg Mannitol:  neg Sorbitol:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  neg Urease:  neg α-Arab:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  + β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  neg Trypsin:  neg AlaPheProAA:  neg CystineAA:  neg ProAA:  neg ValAA:  neg AlkalineP:  neg AcidP:  neg Esterase(C4):  neg EstLip(C8):  w Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    amoxicillin:  SensRNG: (0.03-0.25)
    penicillin:  SensRNG: (0.03-0.5)
    meropenem:  SensRNG: (0.06-2)
    cefotaxime:  Var(MIC50): 0.06-4), MIC90: Var(0.06-4
    cefuroxime:  Var(MIC50): 0.12-8), MIC90: Var(0.12-8
    erythromycin:  Var(MIC50): 0.06-16), MIC90: Var(0.06-16
    quin-dalf:  SensRNG: (1-4)
    levofloxacin:  SensRNG: (0.5-4)
    trovafloxacin:  SensRNG: (0.25-0.5)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    vancomycin:  SensRNG: (0.12-1)
    rifampicin:  SensRNG: (1-4)
    chloramphenicol:  SensRNG: (2-8)
    SXT:  Var(MIC50): 0.12-8), MIC90: Var(0.12-8
    clindamycin:  SensRNG: (0.06-2)

    References

    SPECIFIC REFERENCES FOR RUOFFIA TABACINASALIS
  • Collins1999a - Facklamia tabacinasalis sp. nov., from powdered tobacco.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • LaClaire2000 - Antimicrobial susceptibilities and clinical sources of Facklamia species.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR RUOFFIA TABACINASALIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • Pfleiderer2013 - Culturomics identified 11 new bacterial species from a single anorexia nervosa stool sample.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
  • ...............................
    • GENERAL REFERENCES FOR RUOFFIA TABACINASALIS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021