Slackia heliotrinireducens

(aka Peptostreptococcus heliotrinreducens)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Slackia heliotrinireducens, (aka Peptostreptococcus heliotrinreducens), is a Gram-positive, non-spore-forming, strictly anaerobic, non-motile, coccus bacterium. It has been detected in at least 5 gut microbiome compilation studies or metastudies. The DNA G+C content is 36.1%. Slackia heliotrinireducens is probably a rare gut coloniser. (Wade1999; Lanigan1976; Ezaki1986)



  • This organism has been recovered from ovine rumen. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Wade1999); (Lanigan1976); (Ezaki1986);
    Character Response
  • Substrates hydrolysed or digested:
  • arginine;
  • 🧂
  • Salt tolerance:
  • tolerates 0-1.0% salt;
  • 💧
  • Bile tolerance:
  • Sensitive
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.4; doesn't tolerate pH 8.2; Grows optimally at pH 6.5-7.0.
  • 🌡
  • Temperature tolerance:
  • grows at 30℃; grows at 46℃; Grows optimally at 40℃.
  • ⥅
  • Substrates assimilated or utilised:
  • formate; fumarate;
  • Active enzymes:
  • Ala arylamidase; arginine dihydrolase; Gly arylamidase; His arylamidase; Leu arylamidase; Phe arylamidase; Pro arylamidase; Ser arylamidase; Tyr arylamidase; Val arylamidase;
    • SPECIAL FEATURES (Wade1999); (Lanigan1976); (Ezaki1986);
    Character Response
  • ✓
  • Metabolites produced:
  • acetate; propionate; butyrate; isovalerate (trace); ammonia; CO₂; H₂;
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • Nitrite:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Lanigan1976); (Goldstein2000a);
    Class Active Resistant
  • Penicillins:
  • amoxicillin-clavulanic acid; ampicillin-sulbactam; imipenem; penicillin; penicillin G; piperacillin-tazobactam; ticarcillin-clavulanic acid;
  • Cephalosporins:
  • cefoxitin;
  • Macrolides:
  • erythromycin;
  • Tetracyclines:
  • tetracycline;
  • Heterocycles:
  • chloramphenicol; metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Coriobacteriia Order:  Eggerthellales Family:  Eggerthellaceae Genus:  Slackia Alt. name:  Peptostreptococcus heliotrinreducens Gram stain:  + O2 Relation.:  strictly anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus
    Health:  Unknown
    Source:  ovine rumen
    DNA G+C(%):  36.1
    Opt. T:  40℃
    Lower T(℃):  30(+)
    High T(℃):  46(+)
    NaCl 0.5-2%:  0-1.0(+)
    Opt. pH:  6.5-7.0
    pH 4.2-5.9:  5.4(neg)
    pH >8:  8.2(neg)
    Bile reaction(%):  S
    		Urea:  neg Gelatin:  neg Starch:  neg Arginine:  + Milk:  neg Meat:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Glu:  neg Gly:  neg His:  neg Ser:  neg Thr:  neg Formate:  + Fumarate:  + Lactate:  neg Malate:  neg Pyruvate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  neg Urease:  neg G6PDH6PGDH:  neg α-Arab:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  + Chymotrypsin:  neg GluDC:  neg Trypsin:  neg AlanineAA:  + ArgAA:  neg CystineAA:  vr GluGluAA:  neg GlyAA:  + HisAA:  + LeuAA:  + LeuGlyAA:  neg ProAA:  + PheAA:  + PyrogluAA:  neg SerAA:  + TyrAA:  + ValAA:  + AlkalineP:  neg AcidP:  neg Esterase(C4):  neg EstLip(C8):  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Acetate:  + Propionate:  + Butyrate:  + Isovalerate:  trace(+) H2S:  neg Ammonia:  + CO2:  + H2:  + Indole:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Augmentin:  S(0.125)
    amp-sulb:  S(MIC50): 0.06, MIC90: 0.5, RNG: (0.06–8)
    penicillin:  S(0.19)
    penicillin_G:  S(10U)
    piper-taz:  S(MIC50): 0.25, MIC90: 2, RNG: (0.06–32)
    tica-clav:  S(MIC50): 0.5, MIC90: 16, RNG: (0.06–128)
    imipenem:  S(MIC50): 0.03, MIC90: 0.5, RNG: (0.015–0.5)
    cefoxitin:  S(MIC50): 1, MIC90: 8, RNG: (0.06–8)
    erythromycin:  S(<0.016)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  S(0.25)
    vancomycin:  S(MIC50): 0.25, MIC90: 1, RNG: (0.06–1)
    chloramphenicol:  S(1.5)
    metronidazole:  S(MIC50): 0.5, MIC90: 2, RNG: (0.06->32)
    clindamycin:  S(MIC50): 0.06, MIC90: 2, RNG: (0.06–16)

    References

    SPECIFIC REFERENCES FOR SLACKIA HELIOTRINIREDUCENS
  • Wade1999 - The family Coriobacteriaceae: reclassification of Eubacterium exiguum (Poco etal. 1996) and Peptostreptococcus heliotrinreducens (Lanigan 1976) as Slackia exigua gen. nov., comb. nov. and Slackia heliotrinireducens gen. nov., comb. nov., and Eubacterium lentum (Prevot 1938) as Eggerthella lenta gen. nov., comb. nov.
  • Lanigan1976 - Peptococcus heliotrinreducans, sp. nov., a cytochrome-producing anaerobe which metabolizes pyrrolizidine alkaloids.
  • Goldstein2000a - Comparative In vitro activities of ertapenem (MK-0826) against 1,001 anaerobes isolated from human intra-abdominal infections.
  • Ezaki1986 - Transfer of Peptococcus heliotrinreducens corrig. to the Genus Peptostreptococcus: Peptostreptococcus heliotrinreducens Lanigan 1983 comb. nov.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR SLACKIA HELIOTRINIREDUCENS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • De2020 - Metagenomic analysis of gut microbiome and resistome of diarrheal fecal samples from Kolkata, India, reveals the core and variable microbiota including signatures of microbial dark matter.
  • New2022 - Collective effects of human genomic variation on microbiome function.
  • Qin2012 - Metagenome-wide association study of gut microbiota in type 2 diabetes
  • Zeller2014 - Potential of fecal microbiota for early-stage detection of colorectal cancer
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    • Added: January 19, 2022