Staphylococcus kloosii

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Staphylococcus kloosii is a Gram-positive, non-spore-forming, aerobic, non-motile, coccus bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 31-32.3%. Staphylococcus kloosii is probably a rare gut coloniser. (Schleifer1984a; Schleifer2011Bergey)



  • This organism has been recovered from human faeces, and animals. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Schleifer1984a); (Schleifer2011Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • urea;
  • 🧂
  • Salt tolerance:
  • tolerates 10(w)% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 25℃; grows at 42℃;
  • H+
  • Acid from carbohydrates usually produced:
  • D-arabinose; fructose; galactose; ribose; melezitose;
  • ±
  • Strain-dependent acid from carbs:
  • arabinose; maltose; trehalose; mannitol;
  • ⥅
  • Substrates assimilated or utilised:
  • fructose; galactose; glucose; glycerol; maltose; mannitol; ribose; trehalose;
  • ±
  • Strain-dependent substrate utilisation:
  • L-arabinose; gentiobiose; lactose; raffinose; xylitol;
  • Active enzymes:
  • alkaline phosphatase; acid phosphatase; catalase; esterase C4; esterase lipase C8; β-galactosidase; α-glucosidase; lipase; pyrrolidine arylamidase; urease;
    • SPECIAL FEATURES (Schleifer1984a); (Schleifer2011Bergey);
    Character Response
  • ±
  • VP test:
  • active for a few strains
  • ±
  • Haemolysis:
  • strain dependent
  • ✗
  • Nitrate:
  • not reduced
  • Pigments:
  • variable
    • RESPONSE TO ANTIBIOTICS (Schleifer1984a);
    Class Active Resistant
  • Penicillins:
  • penicillin G;
  • Macrolides:
  • erythromycin;
  • Heterocycles:
  • sulfamethoxazole;
  • Miscellaneous antibiotics:
  • lincomycin;
  • novobiocin;

  • Macfarlane, S., Furrie, E., Cummings, J. H., & Macfarlane, G. T. (2004). Chemotaxonomic analysis of bacterial populations colonizing the rectal mucosa in patients with ulcerative colitis. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 38(12), 1690–1699.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Staphylococcaceae Genus:  Staphylococcus Gram stain:  + O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus Pigment:  variable
    Health:  Unknown
    Source:  human faeces, and animals
    DNA G+C(%):  31-32.3
    Lower T(℃):  25(+)
    High T(℃):  42(+)
    NaCl >6%:  10(w(+))
    		Aesculin:  vr Urea:  d(+) Gelatin:  neg Arginine:  neg Tween:  80(neg)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  d D-Arabinose:  d(+) Fructose:  + Galactose:  w(+) Glucose:  vr Mannose:  neg Ribose:  + Xylose:  neg Cellubiose:  neg Lactose:  vr Maltose:  d Melezitose:  w(+) Sucrose:  neg Trehalose:  d Turanose:  neg Glycogen:  neg Mannitol:  d Xylitol:  neg Me-α-D-Glc:  neg NAc-α-GA:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  d Fructose:  + D-Fucose:  neg Galactose:  + Glucose:  + Mannose:  neg Rhamnose:  neg Ribose:  + Xylose:  neg Cellubiose:  neg Gentiobiose:  d Lactose:  d Maltose:  + Melezitose:  w Melibiose:  neg Raffinose:  d Sucrose:  neg D-Turanose:  w Trehalose:  + Amygdalin:  neg Arbutin:  neg Glycerol:  + Mannitol:  + Sorbitol:  neg Xylitol:  d
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  + Coagulase:  neg Fibrinolysin:  neg HS nuclease:  neg Hyaluridonase:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg α-Mannosidase:  neg ArgDH:  neg OrnDC:  neg LeuAA:  neg PyrrolidAA:  + AlkalineP:  + AcidP:  + DNAse:  neg Esterase(C4):  + EstLip(C8):  + Lipase:  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Pigment:  variable
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    penicillin_G:  SensRNG: (0.05-0.2)
    erythromycin:  SensRNG: (0.1-0.4)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    sulfamethoxazole:  SensRNG: (2-32)
    lincomycin:  SensRNG: (0.4-0.4)
    novobiocin:  R(16-64)

    References

    SPECIFIC REFERENCES FOR STAPHYLOCOCCUS KLOOSII
  • Schleifer1984a - Staphylococcus arlettae sp. nov., S. equorum sp. nov. and S. kloosii sp. nov.: Three New Coagulase-Negative, Novobiocin-Resistant Species from Animals.
  • Schleifer2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Staphylococcaceae, Genus I. Staphylococcus
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR STAPHYLOCOCCUS KLOOSII
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • MacFarlane2004 - Chemotaxonomic analysis of bacterial populations colonizing the rectal mucosa in patients with ulcerative colitis.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • Woodmansey2004 - Comparison of compositions and metabolic activities of fecal microbiotas in young adults and in antibiotic-treated and non-antibiotic-treated elderly subjects.
  • ...............................
    • GENERAL REFERENCES FOR STAPHYLOCOCCUS KLOOSII
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021