Staphylococcus saccharolyticus

(aka Peptococcus saccharolyticus)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Staphylococcus saccharolyticus, (aka Peptococcus saccharolyticus), is a Gram-positive, non-spore-forming, anaerobic, non-motile, coccus bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 33-34%. Staphylococcus saccharolyticus is probably a rare gut coloniser. (Ezaki1983; KilpperBalz1981; Schleifer2011Bergey)



  • This organism has been recovered from human faeces, clinical sources (blood, bone marrow, infections - CCUG) and skin. The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Ezaki1983); (KilpperBalz1981); (Schleifer2011Bergey);
    Character Response
  • Substrates hydrolysed or digested:
  • arginine;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 15℃; grows at 40-45℃; Grows optimally at 30-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; glucose; mannose; glycerol;
  • Active enzymes:
  • acid phosphatase; catalase;
    • SPECIAL FEATURES (Ezaki1983); (KilpperBalz1981); (Schleifer2011Bergey);
    Character Response
  • ✓
  • Metabolites produced:
  • formate (minor); acetate; lactate (minor); ethanol; ammonia; CO₂;
  • ✗
  • Metabolites not produced:
  • H₂S; indole;
  • ✓
  • VP test:
  • active
  • Lysozyme:
  • growth observed
  • ✗
  • Haemolysis:
  • absent
  • ✓
  • Nitrate:
  • reduced
  • ✗
  • Pigments:
  • not produced
    • RESPONSE TO ANTIBIOTICS (KilpperBalz1981);
    Class Active Resistant
  • Miscellaneous antibiotics:
  • novobiocin;

  • Benno, Y., Sawada, K., & Mitsuoka, T. (1984). The intestinal microflora of infants: composition of fecal flora in breast-fed and bottle-fed infants. Microbiology and Immunology, 28(9), 975–986.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Staphylococcaceae Genus:  Staphylococcus Alt. name:  Peptococcus saccharolyticus Gram stain:  + O2 Relation.:  anaerobic Spore:  No spore Motility:  Sessile Morphology:  Coccus Pigment:  neg
    Health:  Unknown
    Source:  human faeces, clinical sources (blood, bone marrow, infections - CCUG) and skin
    DNA G+C(%):  33-34
    Opt. T:  30-37℃
    Low T(℃):  15(neg)
    High T(℃):  40-45(+)
    		Aesculin:  neg Urea:  vr Gelatin:  neg Starch:  neg Casein:  neg Arginine:  + Hippurate:  neg Meat:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  + Galactose:  neg Glucose:  + Mannose:  + Rhamnose:  neg Ribose:  neg D-Tagatose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Dextrin:  neg Glycogen:  neg Adonitol:  neg D-Arabitol:  neg Glycerol:  + Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Me-α-D-Glc:  neg NAc-α-GA:  neg Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  neg Catalase:  + Urease:  vr Coagulase:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg β-Mannosidase:  neg ArgDH:  vr GluDC:  neg OrnDC:  neg AlanineAA:  neg AlaPheProAA:  neg GluGluAA:  neg GlyAA:  neg LeuAA:  neg LeuGlyAA:  neg PyrrolidAA:  neg AlkalineP:  vr AcidP:  + Esterase(C4):  vr EstLip(C8):  vr Lipase(C14):  vr
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Formate:  minor(+) Acetate:  + Lactate:  minor(+) Ethanol:  + H2S:  neg Ammonia:  + CO2:  + Indole:  neg Pigment:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    novobiocin:  Sens

    References

    SPECIFIC REFERENCES FOR STAPHYLOCOCCUS SACCHAROLYTICUS
  • Brueggemann2019 - Staphylococcus saccharolyticus Isolated From Blood Cultures and Prosthetic Joint Infections Exhibits Excessive Genome Decay.
  • Ezaki1983 - Transfer of Peptococcus indolicus, Peptococcus asaccharolyticus, Peptococcus prevotii, and Peptococcus magnus to the Genus Peptostreptococcus and Proposal of Peptostreptococcus tetradius sp. nov.
  • KilpperBalz1981 - Transfer of Peptococcus saccharolyticus Foubert and Douglas to the genus Staphylococcus: Staphylococcus saccharolyticus (Foubert and Douglas) comb. nov.
  • Schleifer2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Staphylococcaceae, Genus I. Staphylococcus
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR STAPHYLOCOCCUS SACCHAROLYTICUS
  • Benno1984 - The intestinal microflora of infants: composition of fecal flora in breast-fed and bottle-fed infants.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR STAPHYLOCOCCUS SACCHAROLYTICUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 08, 2021