Sutcliffiella cohnii

(aka Bacillus cohnii)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Sutcliffiella cohnii, (aka Bacillus cohnii), is a Gram-positive, spore-forming, aerobic, rod-shaped bacterium. The DNA G+C content is 33.5-35%. Sutcliffiella cohnii is likely to be transient and not a long-term gut coloniser. (Logan2011Bergey; Spanka1993)



  • This organism has been recovered from soil and faeces (Logan2011Bergey). The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. Likely to be transient and not a long-term gut commensal. Robust growth can have positive consequences for gut health.
    • QUIRKS
  • Poor growth at pH7 so unlikely to be a gut coloniser.
    • GENERAL CHARACTERISTICS (Logan2011Bergey); (Spanka1993);
    Character Response
  • Substrates hydrolysed or digested:
  • casein; gelatin; hippurate; starch;
  • 🧂
  • Salt tolerance:
  • tolerates 2.0% salt; tolerates 5.0% salt; doesn't tolerate 10% salt;
  • pH
  • Acidity tolerance:
  • doesn't tolerate pH 5.0; doesn't tolerate pH 6.0-7.0; tolerates pH >9.0;
  • 🌡
  • Temperature tolerance:
  • grows at 6(neg), 10℃; grows at 40℃; doesn't grow at 50℃;
  • H+
  • Acid from carbohydrates usually produced:
  • pullulan;
  • ⥅
  • Substrates assimilated or utilised:
  • hippurate;
  • Active enzymes:
  • catalase; β-galactosidase; oxidase;
    • SPECIAL FEATURES (Logan2011Bergey);
    Character Response
  • ✗
  • VP test:
  • not active
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • NOTES

    Able to hydrolyse 4-Methylumbelliferone glucuronide.  This artificial compound is usually used to detect the presence of the GUS enzyme found in E. coli.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Bacillales Family:  Bacillaceae Genus:  Sutcliffiella Alt. name:  Bacillus cohnii Gram stain:  + O2 Relation.:  aerobic Spore:  Endospore Morphology:  Rod
    Health:   Positive
    Source:  soil and faeces (Logan2011Bergey)
    DNA G+C(%):  33.5-35
    Low T(℃):  6(neg), 10(+)
    Mid T(℃):  40(+)
    High T(℃):  50(neg)
    NaCl 0.5-2%:  2.0(+)
    NaCl 3-5%:  5.0(+)
    NaCl >6%:  10(neg)
    pH 4.2-5.9:  5.0(neg)
    pH 6.0-8.0:  6.0-7.0(neg)
    pH >8:  >9.0(+)
    		Urea:  neg Gelatin:  + Starch:  + Casein:  + Hippurate:  + Tween:  60(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    L-Arabinose:  neg Glucose:  neg Mannose:  neg Glycogen:  neg Starch:  neg Mannitol:  neg Me-Xyloside:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Asp:  neg Hippurate:  +
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + β-Galactosidase:  + Phe deaminase:  neg

    References

    SPECIFIC REFERENCES FOR SUTCLIFFIELLA COHNII
  • Logan2011Bergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Bacillaceae Genus I. Bacillus
  • Spanka1993 - Bacillus cohnii sp. nov., a New, Obligately Alkaliphilic, Oval-Spore-Forming Bacillus Species with Ornithine and Aspartic Acid Instead of Diaminopimelic Acid in the Cell Wall.
  • ...............................
    • GENERAL REFERENCES FOR SUTCLIFFIELLA COHNII
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
    • Added: April 22, 2021