Tsukamurella pulmonis

(aka Tsukamurella spongiae)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Tsukamurella pulmonis, (aka Tsukamurella spongiae), is a Gram-positive, non-spore-forming, aerobic, non-motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 70.4%. Tsukamurella pulmonis is likely to be transient and not a long-term gut coloniser. (Teng2016; Yassin1996; Olson2007)



  • This organism has been recovered from human faeces and clinical sources (sputum, blood, wound, eye - CCUG). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread. It is an opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • GENERAL CHARACTERISTICS (Teng2016); (Yassin1996); (Olson2007);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; urea;
  • 🧂
  • Salt tolerance:
  • tolerates 5% salt;
  • 🌡
  • Temperature tolerance:
  • grows at 24℃; grows at 37℃; doesn't grow at 45℃; Grows optimally at 25-37℃.
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; sucrose; trehalose; mannitol; sorbitol;
  • ⥅
  • Substrates assimilated or utilised:
  • N-acetylglucosamine; arabinose; L-arabinose; d_arabitol; aesculin; fructose; fucose; D-fucose; galactose; gluconate; glucose; glycerol; mannitol; rhamnose; sorbitol; sucrose; trehalose; D-turanose; xylose; alanine; proline; acetate; 1,2-propanediol; 2,3-butanediol; acetamide;
  • ±
  • Strain-dependent substrate utilisation:
  • cellubiose; dulcitol; erythritol;
  • Active enzymes:
  • catalase; β-galactosidase; β-glucosidase; urease;
    • SPECIAL FEATURES
    Character Response
  • Lysozyme:
  • growth observed
  • ✗
  • Nitrate:
  • not reduced
    • RESPONSE TO ANTIBIOTICS (Yassin1996);
    Class Active Resistant
  • Aminoglycosides:
  • streptomycin;
  • Polypep/ketides:
  • rifampicin;
  • Heterocycles:
  • isoniazid;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Corynebacteriales Family:  Tsukamurellaceae Genus:  Tsukamurella Alt. name:  Tsukamurella spongiae Gram stain:  + O2 Relation.:  aerobic Spore:  No spore Motility:  Sessile Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and clinical sources (sputum, blood, wound, eye - CCUG)
    DNA G+C(%):  70.4
    Opt. T:  25-37℃
    Lower T(℃):  24(+)
    Mid T(℃):  37(+)
    High T(℃):  45(neg)
    NaCl 3-5%:  5(+)
    		Aesculin:  + Urea:  + Gelatin:  neg Casein:  neg Tyrosine:  neg Tween:  80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg Fructose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  neg Xylose:  neg Cellubiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Sucrose:  + Trehalose:  + Inulin:  neg Adonitol:  neg Inositol:  neg Mannitol:  + Sorbitol:  +
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  + Fructose:  + Fucose:  + D-Fucose:  + Galactose:  + Glucose:  + Mannose:  neg Rhamnose:  + Ribose:  neg Xylose:  + Cellubiose:  d Lactose:  neg Maltose:  neg Melezitose:  neg Raffinose:  neg Sucrose:  + D-Turanose:  + Trehalose:  + N_Acetyl_glucosamine:  + Adonitol:  neg D-Arabitol:  + Arbutin:  neg Dulcitol:  d Erythritol:  d Aesculin:  + Gluconate:  + Glycerol:  + Inositol:  neg Mannitol:  + Me-α-D-Glc:  neg Methanol:  neg Salicin:  neg Sorbitol:  + Xylitol:  neg Ala:  + Pro:  + Ser:  neg Acetate:  + Adipate:  neg Benzoate:  neg Citrate:  neg 3-Hydroxybenzoate:  neg 4-Hydroxybenzoate:  neg 2-Ketogluconate:  neg Lactate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  + Urease:  + β-Galactosidase:  + β-Glucosidase:  +
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    streptomycin:  Res
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    rifampicin:  Res
    isoniazid:  Res

    References

    SPECIFIC REFERENCES FOR TSUKAMURELLA PULMONIS
  • Nouioui2018 - Genome-Based Taxonomic Classification of the Phylum Actinobacteria.
  • Teng2016 - Phylogenomic Analyses and Reclassification of Species within the Genus Tsukamurella: Insights to Species Definition in the Post-genomic Era.
  • Yassin1996 - Tsukamurella pulmonis sp. nov.
  • Olson2007 - Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marine sponge.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR TSUKAMURELLA PULMONIS
  • Yang2020 - Species-Level Analysis of Human Gut Microbiota With Metataxonomics.
  • Yang2020a - Establishing high-accuracy biomarkers for colorectal cancer by comparing fecal microbiomes in patients with healthy families
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    • Added: December 15, 2021