Ureibacillus thermosphaericus

(aka Bacillus thermosphaericus)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Ureibacillus thermosphaericus, (aka Bacillus thermosphaericus), is a Gram-negative, spore-forming, strictly aerobic, motile, bacterium. It has been detected in at least 4 gut microbiome compilation studies or metastudies. The DNA G+C content is 36-37%. Ureibacillus thermosphaericus is unlikely to be a gut coloniser. (Fortina2001; Fritze2011aBergey; Andersson1995)



  • This organism has been recovered from human faeces (Hoyles2012), clinical sources (cerebrospinal fluid, blood - CCUG), air sample, and landfill waste. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. It is an opportunistic pathogen. Likely to be transient and not a long-term gut commensal.
    • QUIRKS
  • Obligate aerobe and high salt required for optimum growth, so likely a gut non-coloniser.
    • GENERAL CHARACTERISTICS (Fortina2001); (Fritze2011aBergey); (Andersson1995);
    Character Response
  • Substrates hydrolysed or digested:
  • aesculin; urea;
  • 🧂
  • Salt tolerance:
  • tolerates 5% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 7; tolerates pH 9;
  • 🌡
  • Temperature tolerance:
  • grows at 33℃; grows at 64℃; Grows optimally at 60℃.
  • ⥅
  • Substrates assimilated or utilised:
  • aesculin; malate;
  • Active enzymes:
  • esterase C4; esterase lipase C8;
    • SPECIAL FEATURES (Fritze2011aBergey); (Andersson1995);
    Character Response
  • ✗
  • Metabolites not produced:
  • indole;
  • ✗
  • VP test:
  • not active
  • ✗
  • Nitrate:
  • not reduced

  • Lagier, J.-C., Armougom, F., Million, M., Hugon, P., Pagnier, I., Robert, C., Bittar, F., Fournous, G., Gimenez, G., Maraninchi, M., Trape, J.-F., Koonin, E. V., La Scola, B., & Raoult, D. (2012). Microbial culturomics: paradigm shift in the human gut microbiome study. Clinical Microbiology and Infection: The Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(12), 1185–1193.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Firmicutes Class:  Bacilli Order:  Caryophanales Family:  Caryophanaceae Genus:  Ureibacillus Alt. name:  Bacillus thermosphaericus Gram stain:  neg O2 Relation.:  strictly aerobic Spore:  Endospore Motility:  Swimming
    Health:  Unknown
    Source:  human faeces (Hoyles2012), clinical sources (cerebrospinal fluid, blood - CCUG), air sample, and landfill waste
    DNA G+C(%):  36-37
    Opt. T:  60℃
    Mid T(℃):  33(+)
    High T(℃):  64(+)
    NaCl 3-5%:  5(+)
    pH 6.0-8.0:  7(+)
    pH >8:  9(+)
    		Aesculin:  + Urea:  + Gelatin:  neg Starch:  neg Casein:  neg Arginine:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  neg D-Arabinose:  neg L-Arabinose:  neg Fructose:  neg D-Fucose:  neg Galactose:  neg Glucose:  neg Mannose:  neg D-Lyxose:  neg Rhamnose:  neg Ribose:  neg Sorbose:  neg D-Tagatose:  neg Xylose:  neg L-Xylose:  neg Cellubiose:  neg Gentiobiose:  neg Lactose:  neg Maltose:  neg Melezitose:  neg Melibiose:  neg Sucrose:  neg Trehalose:  neg Turanose:  neg Amygdalin:  neg Glycogen:  neg Inulin:  neg Starch:  neg Adonitol:  neg D-Arabitol:  neg L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Inositol:  neg Mannitol:  neg Sorbitol:  neg Xylitol:  neg Arbutin:  neg Gluconate:  neg 2-Ketogluconate:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Me-α-D-Mann:  neg Me-Xyloside:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  neg Glucose:  neg Mannose:  neg Maltose:  neg N_Acetyl_glucosamine:  neg Aesculin:  + Gluconate:  neg Mannitol:  neg Adipate:  neg Caprate:  neg Citrate:  neg Malate:  + Phenylacetate:  neg D-Tartrate:  neg L-Tartrate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg γ-Glu transf.:  neg OrnDC:  neg Phe deaminase:  neg LeuAA:  neg AlkalineP:  neg AcidP:  neg Esterase(C4):  + EstLip(C8):  + Lipase:  neg Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		Indole:  neg

    References

    SPECIFIC REFERENCES FOR UREIBACILLUS THERMOSPHAERICUS
  • Fortina2001 - Ureibacillus gen. nov., a new genus to accommodate Bacillus thermosphaericus (Andersson et al. 1995), emendation of Ureibacillus thermosphaericus and description of Ureibacillus terrenus sp. nov.
  • Fritze2011aBergey - Bergey's manual of systematic bacteriology. Vol. 3, The Firmicutes. Family Planococcaceae, Genus IX. Ureibacillus
  • Andersson1995 - Bacillus thermosphaericus sp. nov. a New Thermophilic Ureolytic.
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR UREIBACILLUS THERMOSPHAERICUS
  • Byrd2020 - Stability and dynamics of the human gut microbiome and its association with systemic immune traits.
  • Hoyles2012 - Recognition of greater diversity of Bacillus species and related bacteria in human faeces.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR UREIBACILLUS THERMOSPHAERICUS
  • Ludwig2009 - Revised road map to the phylum Firmicutes.
  • Hoyles2012 - Recognition of greater diversity of Bacillus species and related bacteria in human faeces.
    • Added: April 08, 2021