Variovorax boronicumulans

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Variovorax boronicumulans is a Gram-negative, non-spore-forming, motile, rod-shaped bacterium. It has been detected in at least 1 gut microbiome compilation study or metastudy. The DNA G+C content is 71.2%. Variovorax boronicumulans is probably a rare gut coloniser. (Miwa2008)



  • This organism has been recovered from human faeces, and soil. The risk classification (www.baua.de) for this organism is 1, i.e., low risk of infection and spread. Pathogenicity status unknown, or very unlikely to be pathogenic. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Miwa2008);
    Character Response
  • 🧂
  • Salt tolerance:
  • tolerates 1% salt;
  • pH
  • Acidity tolerance:
  • tolerates pH 5; tolerates pH 6.0-8.0; tolerates pH 9; Grows optimally at pH 7.
  • 🌡
  • Temperature tolerance:
  • grows at 4℃; grows at 37℃; doesn't grow at 45℃; Grows optimally at 30℃.
  • ⥅
  • Substrates assimilated or utilised:
  • citrate;
  • Active enzymes:
  • alkaline phosphatase; catalase; esterase lipase C8; Leu arylamidase; oxidase;
    • SPECIAL FEATURES (Miwa2008);
    Character Response
  • ✗
  • Metabolites not produced:
  • Hâ‚‚S; indole;
  • ✓
  • VP test:
  • active
  • ✗
  • Nitrate:
  • not reduced

  • A non-spore-forming, Gram-negative, motile, rod-shaped, boron-accumulating bacterium isolated from soil was characterized by polyphasic taxonomy. The strain, designated BAM-48(T), was positive for catalase and oxidase activities and grew at 4-37 degrees C, 0-1% NaCl and pH 5-9. Comparative analysis of the 16S rRNA gene sequence demonstrated that the novel isolate BAM-48(T) was closely related to Variovorax paradoxus IAM 12373(T) (99.3% sequence similarity), Variovorax soli GH9-3(T) (98.3%) and Variovorax dokdonensis DS-43(T) (97.0%). DNA-DNA hybridization values between strain BAM-48(T) and V. paradoxus NBRC 15149(T), V. soli KACC 11579(T) and V. dokdonensis KCTC 12544(T) were only 49.1, 25.4 and 24.6%, respectively. The major respiratory quinone was ubiquinone 8 (Q-8). The DNA G+C content was 71.2 mol%. Strain BAM-48(T) contained C(16:0) (36.1%), C(16:1)omega7c (21.4%) and C(17:0) cyclo (19.0%) as the major fatty acids. Based on phenotypic, chemotaxonomic and phylogenetic data, strain BAM-48(T) should be classified as a member of a novel species in the genus Variovorax, for which the name Variovorax boronicumulans sp. nov. is proposed

  • McLaughlin, S. D., Walker, A. W., Churcher, C., Clark, S. K., Tekkis, P. P., Johnson, M. W., Parkhill, J., Ciclitira, P. J., Dougan, G., Nicholls, R. J., & Petrovska, L. (2010). The bacteriology of pouchitis: a molecular phylogenetic analysis using 16S rRNA gene cloning and sequencing. Annals of Surgery, 252(1), 90–98.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Betaproteobacteria Order:  Burkholderiales Family:  Comamonadaceae Genus:  Variovorax Gram stain:  neg Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces, and soil
    DNA G+C(%):  71.2
    Opt. T:  30℃
    Low T(℃):  4(+)
    Mid T(℃):  37(+)
    High T(℃):  45(neg)
    NaCl 0.5-2%:  1(+)
    Opt. pH:  7
    pH 4.2-5.9:  5(+)
    pH 6.0-8.0:  6.0-8.0(+)
    pH >8:  9(+)
    		Aesculin:  neg Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Glucose:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    L-Arabinose:  w Glucose:  neg Mannose:  neg Rhamnose:  neg Maltose:  neg Melibiose:  neg Sucrose:  neg N_Acetyl_glucosamine:  neg Amygdalin:  neg Gluconate:  neg Inositol:  neg Mannitol:  w Sorbitol:  neg Trp:  neg Adipate:  neg Caprate:  w Citrate:  + Malate:  w Phenylacetate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Catalase:  + Urease:  neg Ac-β-glcamnd:  neg α-Fucosidase:  neg α-Galactosidase:  neg β-Galactosidase:  neg α-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  neg ArgDH:  neg Chymotrypsin:  neg OrnDC:  neg Trypsin:  neg TrpAA:  neg CystineAA:  neg LeuAA:  + ValAA:  neg AlkalineP:  + AcidP:  w Esterase(C4):  neg EstLip(C8):  + Lipase(C14):  neg
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  neg

    References

    SPECIFIC REFERENCES FOR VARIOVORAX BORONICUMULANS
  • Miwa2008 - Variovorax boronicumulans sp. nov., a boron-accumulating bacterium isolated from soil.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR VARIOVORAX BORONICUMULANS
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
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    • Added: April 08, 2021