Vibrio fluvialis

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Vibrio fluvialis is a Gram-negative, non-spore-forming, facultatively anaerobic, motile, rod-shaped bacterium. It has been detected in at least 2 gut microbiome compilation studies or metastudies. The DNA G+C content is 49.3-50.6%. Vibrio fluvialis is probably a rare gut coloniser. (Brayton1986; Brenner1983; Lee1981; Farmer2005gBergey)



  • This organism has been recovered from human faeces and clinical sources (wound, diarrhoea - CCUG). The risk classification (www.baua.de) for this organism is 2, i.e., risk of individual infection, but low risk of spread (notes: zoonotic pathogen). It is an opportunistic pathogen. A possible gut commensal.
    • QUIRKS
  • Data combined for biovars I and II, plus other strains.
    • GENERAL CHARACTERISTICS (Brayton1986); (Brenner1983); (Lee1981); (Farmer2005gBergey);
    Character Response
  • Substrates hydrolysed or digested:
  • arginine; casein; chitin; DNA; gelatin; starch; tyrosine;
  • 🧂
  • Salt tolerance:
  • tolerates 1% salt; tolerates 10% salt;
  • 🌡
  • Temperature tolerance:
  • doesn't grow at 5℃; grows at 37℃; doesn't grow at 43℃;
  • H+
  • Acid from carbohydrates usually produced:
  • arabinose; L-arabinose; galactose; glucose; amygdalin; maltose; sucrose; trehalose; D-arabitol; mannitol;
  • ±
  • Strain-dependent acid from carbs:
  • mannose; cellubiose;
  • ⥅
  • Substrates assimilated or utilised:
  • arabinose; L-arabinose; ethanol; fructose; galactose; gluconate; glucose; glycerol; maltose; mannitol; mannose; salicin; sucrose; trehalose; alanine; D-alanine; γ-aminobutyrate; arginine; aspartate; L-citrulline; glycine; histidine; proline; serine; threonine; tyrosine; acetate; citrate; glutarate; 3-hydroxybutyrate; 3-hydroxybenzoate; 2-ketoglutarate; DL-lactate; malate; L-malate; propionate; pyruvate; succinate; L-asparagine; putrescine;
  • ±
  • Strain-dependent substrate utilisation:
  • cellubiose; δ-aminovalerate; D-glucuronate;
  • Active enzymes:
  • arginine dihydrolase; DNase; β-galactosidase; lecithinase; lipase; oxidase;
    • SPECIAL FEATURES (Brayton1986); (Brenner1983); (Lee1981); (Farmer2005gBergey);
    Character Response
  • ✓
  • Metabolites produced:
  • indole (strain-dependent);
  • ✗
  • Metabolites not produced:
  • H₂S;
  • ✓
  • Methyl red test:
  • active
  • ✗
  • VP test:
  • not active
  • ±
  • ONPG test:
  • strain-dependent and weak
  • ±
  • KCN growth:
  • strain-dependent
  • ✓
  • Haemolysis:
  • present
  • ✓
  • Nitrate:
  • reduced
  • ✓
  • NO3➔NO2:
  • reduced
  • ✓
  • Thiosulfate:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Brayton1986); (Brenner1983); (Lee1981); (Farmer2005gBergey);
    Class Active Resistant
  • Penicillins:
  • ampicillin; carbenicillin; penicillin; penicillin G;
  • Quinolines:
  • nalidixic-acid;
  • Aminoglycosides:
  • gentamicin; kanamycin;
  • Heterocycles:
  • trimethoprim;
  • Miscellaneous antibiotics:
  • colistin;
  • novobiocin;

  • Lee, J. V., Shread, P., Furniss, A. L., & Bryant, T. N. (1981). Taxonomy and description of Vibrio fluvialis sp. nov. (synonym group F vibrios, group EF6). The Journal of Applied Bacteriology, 50(1), 73–94.

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Proteobacteria Class:  Gammaproteobacteria Order:  Vibrionales Family:  Vibrionaceae Genus:  Vibrio Gram stain:  neg O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Swimming Morphology:  Rod
    Health:  Unknown
    Source:  human faeces and clinical sources (wound, diarrhoea - CCUG)
    DNA G+C(%):  49.3-50.6
    Low T(℃):  5(neg)
    Mid T(℃):  37(+)
    High T(℃):  43(neg)
    NaCl 0.5-2%:  1(+)
    NaCl >6%:  10(+)
    		Aesculin:  neg Urea:  neg Gelatin:  + Starch:  + Casein:  + Arginine:  + Alginate:  neg Chitin:  + DNA:  + Tyrosine:  + Hippurate:  neg Tween:  80(+)
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    Arabinose:  + L-Arabinose:  + Galactose:  + Glucose:  + Mannose:  d Rhamnose:  neg Xylose:  neg Cellubiose:  d(neg) Lactose:  neg Maltose:  + Melibiose:  neg Sucrose:  + Trehalose:  + Amygdalin:  + Adonitol:  neg D-Arabitol:  d(+) L-Arabitol:  neg Dulcitol:  neg Erythritol:  neg Glycerol:  neg Inositol:  neg Mannitol:  + Sorbitol:  neg 5-Ketogluconate:  neg Me-α-D-Glc:  neg Salicin:  neg
    SUBSTRATE ASSIMILATION & UTILISATION
    Monosaccharide util/assim Oligosaccharide util/assim Other carboh. util/assim Amino acid util/assim Organic acid util/assim
    Arabinose:  + L-Arabinose:  + Fructose:  + Galactose:  + Glucose:  + Mannose:  + Rhamnose:  neg Sorbose:  neg Xylose:  neg Cellubiose:  d Lactose:  neg Maltose:  + Melezitose:  neg Melibiose:  neg Raffinose:  neg Sucrose:  + Trehalose:  + Adonitol:  neg Dulcitol:  neg Erythritol:  neg Ethanol:  + Gluconate:  + Glycerol:  + Inositol:  neg Mannitol:  + Salicin:  + Sorbitol:  neg Ala:  + D-Ala:  + β-Ala:  neg 4-Aminobutyrate:  + 5-Aminovalerate:  d(neg) Arg:  + Asp:  + Betaine:  neg L-Citrulline:  + Glu:  neg Gly:  + His:  + Ile:  neg Leu:  neg Lys:  neg Met:  neg Phe:  neg Pro:  + Sarcosine:  neg Ser:  + Thr:  + Trp:  neg Tyr:  + Val:  neg Acetate:  + Benzoate:  neg Butyrate:  neg Citrate:  + Formate:  neg Glutarate:  + Heptanoate:  neg 3-Hydroxybutyrate:  + 3-Hydroxybenzoate:  + 4-Hydroxybenzoate:  neg DL-Lactate:  + Malate:  + L-Malate:  + Malonate:  neg Mucate:  neg Nonanoate:  neg Propionate:  + Pyruvate:  + Succinate:  + Valerate:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Oxidase:  + Urease:  neg Ac-β-glcamnd:  neg α-Galactosidase:  neg β-Galactosidase:  + α-Glucosidase:  neg β-Glucuronidase:  neg ArgDH:  + LysDC:  neg OrnDC:  neg Phe deaminase:  neg DNAse:  + Lecithinase:  + Lipase:  +
    METABOLITES - PRODUCTION & USE
    Fuel Usable Metabolites Metabolites Released Special Products Compounds Produced

    		H2S:  neg Indole:  (d)
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  R(16->16)
    carbenicil:  R(32->256)
    penicillin:  R(10U)
    penicillin_G:  Res
    cephalothin:  Var(MIC50): 30; disc), MIC90: Var(30; disc
    gentamicin:  S(10; disc)
    kanamycin:  S(30; disc)
    streptomycin:  Var(MIC50): 10; disc), MIC90: Var(10; disc
    nalidixic-acid:  S(30; disc)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    tetracycline:  Var(MIC50): 30; disc), MIC90: Var(30; disc
    chloramphenicol:  Var(MIC50): 30; disc), MIC90: Var(30; disc
    sulfadiazine:  Var(MIC50): 250; disc), MIC90: Var(250; disc
    trimethoprim:  S(1.25; disc)
    novobiocin:  R(5; disc)
    colistin:  S(10; disc)
    polymyxin_B:  RNG: (2-8)
    vibriostat_129:  RNG: (20-160)

    References

    SPECIFIC REFERENCES FOR VIBRIO FLUVIALIS
  • Brayton1986 - Vibrio cincinnatiensis sp. nov., a new human pathogen.
  • Brenner1983 - Vibrio furnissii (formerly aerogenic biogroup of Vibrio fluvialis), a new species isolated from human feces and the environment.
  • Lee1981 - Taxonomy and Description of Vibrio fluvialis sp. nov. (Synonym Group F Vibrios, Group EF6).
  • Farmer2005gBergey - Bergey's manual of systematic bacteriology. Vol. 2, The Gammaproteobacteria Part B. Family Vibrionaceae, Genus I. Vibrio
  • ...............................
    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR VIBRIO FLUVIALIS
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
  • RajilicStojanovic2014 - The first 1000 cultured species of the human gastrointestinal microbiota.
  • ...............................
    • GENERAL REFERENCES FOR VIBRIO FLUVIALIS
  • CCUG - Culture Collection University of Gothenburg - Entire Collection
    • Added: April 08, 2021