Winkia neuii

(aka Actinomyces neuii)

Bacteria
General | Carbohydrate O/F | Substrate utilisation | Enzymes | Metabolites | Antibiotics

Overview

  • Winkia neuii, (aka Actinomyces neuii), is a Gram-positive, non-spore-forming, facultatively anaerobic, non-motile, diphtheroidal bacterium. It has been detected in at least 3 gut microbiome compilation studies or metastudies. The DNA G+C content is 55-58%. Winkia neuii is probably a rare gut coloniser. (Funke1994)



  • This organism has been recovered from human faeces and clinical sources (blood, wound, abscess, vagina, urine - CCUG). It is an opportunistic pathogen. A possible gut commensal.
    • GENERAL CHARACTERISTICS (Funke1994);
    Character Response
  • H+
  • Acid from carbohydrates usually produced:
  • fructose; galactose; glucose; mannose; ribose; xylose; lactose; maltose; D-lyxose; melezitose; melibiose; raffinose; sucrose; trehalose; D-arabitol; erythritol; glycerol; inositol; mannitol; 5-ketogluconate;
  • ±
  • Strain-dependent acid from carbs:
  • fucose; D-fucose; D-tagatose; glycogen; inulin; cellubiose; gentiobiose; adonitol; L-arabitol; dulcitol; xylitol; gluconate; α-methyl glucoside; methyl mannoside; N-Ac glucosamine;
  • Active enzymes:
  • catalase; α-galactosidase; β-galactosidase; α-glucosidase; Leu arylamidase; α-mannosidase;
    • SPECIAL FEATURES
    Character Response
  • Haemolysis:
  • alpha
  • ✓
  • NO3➔NO2:
  • reduced
    • RESPONSE TO ANTIBIOTICS (Tyrrell2012); (Goldstein2006c); (Goldstein2000);
    Class Active Resistant
  • Penicillins:
  • ampicillin; imipenem; meropenem; penicillin G; piperacillin-tazobactam;
  • Macrolides:
  • azithromycin; erythromycin;
  • Tetracyclines:
  • doxycycline; minocycline; tetracycline; tigecycline;
  • Quinolines:
  • levofloxacin; moxifloxacin;
  • Heterocycles:
  • metronidazole;
  • Vancomycins:
  • vancomycin;
  • Miscellaneous antibiotics:
  • clindamycin; linezolid;

    • Details

    GENERAL
    Lineage Physiology General Growth Tolerances Hydrol./digest./degr.
    Phylum:  Actinobacteria Class:  Actinomycetia Order:  Actinomycetales Family:  Actinomycetaceae Genus:  Winkia Alt. name:  Actinomyces neuii Gram stain:  + O2 Relation.:  facultatively anaerobic Spore:  No spore Motility:  Sessile Morphology:  Diphtheroidal
    Health:  Unknown
    Source:  human faeces and clinical sources (blood, wound, abscess, vagina, urine - CCUG)
    DNA G+C(%):  55-58
    		Aesculin:  neg Urea:  neg Gelatin:  neg
    CARBOHYDRATE ACID FORMATION
    Monosaccharide O/F Oligosaccharide O/F Polysaccharide O/F Polyol O/F Other O/F
    D-Arabinose:  neg Fructose:  + Fucose:  d D-Fucose:  d Galactose:  + Glucose:  + Mannose:  + D-Lyxose:  + Rhamnose:  neg Ribose:  + Sorbose:  neg D-Tagatose:  d Xylose:  + L-Xylose:  neg Cellubiose:  d Gentiobiose:  d Lactose:  + Maltose:  + Melezitose:  + Melibiose:  + Sucrose:  + Trehalose:  + Glycogen:  d Inulin:  d Adonitol:  d D-Arabitol:  + L-Arabitol:  d Dulcitol:  d Erythritol:  + Glycerol:  + Inositol:  + Mannitol:  + Xylitol:  d Gluconate:  d 2-Ketogluconate:  neg 5-Ketogluconate:  + Me-α-D-Glc:  d Me-α-D-Mann:  d NAc-α-GA:  d Salicin:  neg
    ENZYME ACTIVITY
    Enzymes: General Enzymes: Carbohydrate Enzymes: Protein Enzymes: Arylamidases Enzymes: Esters/fats
    Catalase:  + Urease:  neg α-Galactosidase:  + β-Galactosidase:  + α-Glucosidase:  + β-Glucosidase:  neg β-Glucuronidase:  neg α-Mannosidase:  + Chymotrypsin:  neg Trypsin:  neg LeuAA:  + ValAA:  neg AlkalineP:  neg
    ANTIBIOTICS ℞
    Penicillins & Penems (μg/mL) Cephalosporins (μg/mL) Aminoglycosides (μg/mL) Macrolides (μg/mL) Quinolones (μg/mL)
    ampicillin:  S(MIC50): 0.125, MIC90: 0.25, RNG: (0.03–1)
    penicillin_G:  S(MIC50): 0.03, MIC90: 0.25, RNG: (≤0.015–0.5)
    piper-taz:  S(MIC50): 0.25, MIC90: 1, RNG: (0.03–2)
    imipenem:  S(MIC50): 0.06, MIC90: 0.125, RNG: (0.015–0.5)
    meropenem:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.015–0.5)
    azithromycin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.03–>32)
    erythromycin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.03–.128)
    linezolid:  S(MIC50): 0.5, MIC90: 1, RNG: (0.125–1)
    levofloxacin:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.125–1)
    moxifloxacin:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.06–1)
    Tetracyclines (μg/mL) Vancomycin Class (μg/mL) Polypep/ketides (μg/mL) Heterocycles (μg/mL) Other (μg/mL)
    doxycycline:  S(MIC50): 0.25, MIC90: 0.5, RNG: (0.06–1)
    minocycline:  S(MIC50): 0.125, MIC90: 0.25, RNG: (0.06–1)
    tetracycline:  S(MIC50): 1, MIC90: 2, RNG: (0.5–4)
    tigecycline:  S(MIC50): 0.125, MIC90: 0.5, RNG: (0.03–1)
    vancomycin:  S(MIC50): 0.5, MIC90: 1, RNG: (0.25–1.0)
    metronidazole:  R(MIC50): >16, MIC90: >16, RNG: (1–>16)
    clindamycin:  S(MIC50): 0.06, MIC90: 0.25, RNG: (0.03->32)
    daptomycin:  Var(MIC50): 2, MIC90: 32, RNG: (0.03->32)

    References

    SPECIFIC REFERENCES FOR WINKIA NEUII
  • Nouioui2018 - Genome-Based Taxonomic Classification of the Phylum Actinobacteria.
  • Tyrrell2012 - In vitro activity of TD-1792, a multivalent glycopeptide-cephalosporin antibiotic, against 377 strains of anaerobic bacteria and 34 strains of Corynebacterium species.
  • Goldstein2006c - Comparative in vitro susceptibilities of 396 unusual anaerobic strains to tigecycline and eight other antimicrobial agents.
  • Goldstein2000 - Comparative in vitro activities of GAR-936 against aerobic and anaerobic animal and human bite wound pathogens.
  • Funke1994 - Assignment of human-derived CDC group 1 coryneform bacteria and CDC group 1-like coryneform bacteria to the genus Actinomyces as Actinomyces neuii subsp. neuii sp. nov., subsp. nov., and Actinomyces neuii subsp. anitratus subsp. nov.
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    • GUT MICROBIOME COMPILATIONS AND METASTUDIES FOR WINKIA NEUII
  • Almeida2019 - A new genomic blueprint of the human gut microbiota.
  • Forster2019 - A human gut bacterial genome and culture collection for improved metagenomic analyses.
  • Lagier2016 - Culture of previously uncultured members of the human gut microbiota by culturomics.
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    • Added: December 15, 2021