Pu•biome

Notes

A Gram-stain-negative bacterium, designated strain Marseille-Q3039T, was isolated from subgingival dental plaque of a woman with gingivitis in Marseille, France. Strain Marseille-Q3039T was found to be an anaerobic, motile and spore-forming crescent-shaped bacterium that grew at 25&ndash;41.5&thinsp;&deg;C (optimum, 37&thinsp;&deg;C), pH 5.5&ndash;8.5 (optimum, pH 7.5) and salinity of 5.0&thinsp;g l&minus;1 NaCl. The results of 16S rRNA gene sequence analysis revealed that strain Marseille-Q3039T was closely related to <a class="namesforlife" title="Selenomonas infelix - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4427" target="xrefwindow" rel="namesforlife-name">Selenomonas infelix</a> ATCC 43532T (98.42 % similarity), <a class="namesforlife" title="Selenomonas dianae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4425" target="xrefwindow" rel="namesforlife-name">Selenomonas dianae</a> ATCC 43527T (97.25 %) and Centipedia periodontii DSM 2778T (97.19 %). The orthologous average nucleotide identity and digital DNA&ndash;DNA hybridization relatedness between strain Q3039T and its closest phylogenetic neighbours were respectively 84.57 and 28.2 % for <a class="namesforlife" title="S. infelix - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4427" target="xrefwindow" rel="namesforlife-name">S. infelix</a> ATCC 43532T and 83.93 and 27.2 % for <a class="namesforlife" title="C. periodontii - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4392" target="xrefwindow" rel="namesforlife-name">C. periodontii</a> DSM 2778T. The major fatty acids were identified as C13 : 0 (27.7 %), C15 : 0 (24.4 %) and specific C13 : 0 3-OH (12.3 %). Genome sequencing revealed a genome size of 2 351 779&thinsp;bp and a G+C content of 57.2 mol%. On the basis of the results from phenotypic, chemotaxonomic, genomic and phylogenetic analyses and data, we concluded that strain Marseille-Q3039T represents a novel species of the genus <a class="namesforlife" title="Selenomonas - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4421" target="xrefwindow" rel="namesforlife-name">Selenomonas</a> , for which the name Selenomonas timonae sp. nov. is proposed (=CSUR Q3039=CECT 30128).

Update: Antezack2021