Pu•biome

Notes

A novel nitrogen-fixing fermentative bacterium, designated as YA01T, was isolated from Nakabusa hot springs in Japan. The short-rod cells of strain YA01T were Gram-positive and non-sporulating. Phylogenetic trees of the 16S rRNA gene sequence and concatenated sequences of 40 single-copy ribosomal genes revealed that strain YA01T belonged to the genus <a class="namesforlife" title="Caldicellulosiruptor - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4480" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor</a> and was closely related to <a class="namesforlife" title="Caldicellulosiruptor hydrothermalis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13259" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor hydrothermalis</a> 108T, <a class="namesforlife" title="Caldicellulosiruptor bescii - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.20088" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor bescii</a> DSM 6725T and <a class="namesforlife" title="Caldicellulosiruptor kronotskyensis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13260" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor kronotskyensis</a> 2002T. The 16S rRNA gene sequence of strain YA01T shares less than 98.1 % identity to the known <a class="namesforlife" title="Caldicellulosiruptor - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4480" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor</a> species. The G+C content of the genomic DNA was 34.8&thinsp;mol%. Strain YA01T shares low genome-wide average nucleotide identity (90.31&ndash;91.10 %), average amino acid identity (91.45&ndash;92.10 %) and &lt;70 % digital DNA&ndash;DNA hybridization value (41.8&ndash;44.2 %) with the three related species of the genus <a class="namesforlife" title="Caldicellulosiruptor - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4480" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor</a> . Strain YA01T grew at 50&ndash;78&thinsp;&deg;C (optimum, 70&thinsp;&deg;C) and at pH 5.0&ndash;9.5 (optimum, pH 6.5). Strain YA01T mainly produced acetate by consuming d(+)-glucose as a carbon source. The main cellular fatty acids were iso-C17 : 0 (35.7 %), C16 : 0 (33.3 %), DMA16 : 0 (6.6 %) and iso-C15 : 0 (5.9 %). Based on its distinct phylogenetic position, biochemical and physiological characteristics, and the major cellular fatty acids, strain YA01T is considered to represent a novel species of the genus <a class="namesforlife" title="Caldicellulosiruptor - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4480" target="xrefwindow" rel="namesforlife-name">Caldicellulosiruptor</a> for which the name Caldicellulosiruptor diazotrophicus sp. nov. is proposed (type strain YA01T=DSM 112098T=JCM 34253T).

Update: Chen2021