Pu•biome

Notes

Four unknown strains, characterized as Gram-stain-negative, strictly anaerobic, non-motile and rod-shaped, were isolated from fresh faeces of healthy humans in PR China. Pairwise sequence comparisons of the 16S rRNA genes showed that these isolates were separated into two clusters. Cluster I (strains HF-5141T and HF-106) was most closely related to <a class="namesforlife" title="Bacteroides xylanisolvens - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13240" target="xrefwindow" rel="namesforlife-name">Bacteroides xylanisolvens</a> XB1AT (98.0&ndash;98.3 %&thinsp;similarity) and <a class="namesforlife" title="Bacteroides ovatus - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.7973" target="xrefwindow" rel="namesforlife-name">Bacteroides ovatus</a> ATCC 8483T (97.3&ndash;97.5 %), whereas cluster II (strains HF-5287T and HF-5300) exhibited a similarity range of 96.8&ndash;97.0 % to <a class="namesforlife" title="Bacteroides finegoldii - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.10054" target="xrefwindow" rel="namesforlife-name">Bacteroides finegoldii</a> JCM 13345T, 96.7&ndash;96.9 % to <a class="namesforlife" title="Bacteroides faecis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.20230" target="xrefwindow" rel="namesforlife-name">Bacteroides faecis</a> MAJ27T and 96.4&ndash;96.5 % to <a class="namesforlife" title="Bacteroides xylanisolvens - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13240" target="xrefwindow" rel="namesforlife-name">Bacteroides xylanisolvens</a> XB1AT. The DNA G+C contents of type strains HF-5141T and HF-5287T were 41.5 and 42.6&thinsp;mol%, respectively. These strains had anteiso-C15 : 0 as the major cellular fatty acid, MK-9 and MK-11 as the predominant respiratory quinones, and phosphatidylethanolamine, aminophospholipids and phospholipids as major polar lipids, which is typical for members of the genus <a class="namesforlife" title="Bacteroides - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.7931" target="xrefwindow" rel="namesforlife-name">Bacteroides</a> . However, the average nucleotide identity and digital DNA&ndash;DNA hybridization values, accompanied by different phenotypic and biochemical characteristics, distinguished them from their corresponding closest relatives as well as from other recognized members of the genus <a class="namesforlife" title="Bacteroides - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.7931" target="xrefwindow" rel="namesforlife-name">Bacteroides</a> . Therefore, strains HF-5141T and HF-5287T represent two novel species in the genus <a class="namesforlife" title="Bacteroides - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.7931" target="xrefwindow" rel="namesforlife-name">Bacteroides</a> , for which the names Bacteroides luhongzhouii sp. nov. and Bacteroides zhangwenhongii sp. nov. are proposed, with HF-5141T (=CGMCC 1.16787T=GDMCC 1.1591T=JCM 33480T) and HF-5287T (=CGMCC 1.16724T=GDMCC 1.1590T=JCM 33481T) as type strains.

Update: Ge2021