Pu•biome

Notes

Four obligatory anaerobic, Gram-stain-positive, non-motile and rod-shaped organisms (HF-1365T, HF-1362, HF-1101T and HF-4214) were isolated from faecal samples of healthy Chinese subjects. Results of 16S rRNA gene sequence analyses showed that these isolates belong to the genera <a class="namesforlife" title="Enorma - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.24643" target="xrefwindow" rel="namesforlife-name">Enorma</a> (strains HF-1365T and HF-1362) and <a class="namesforlife" title="Eggerthella - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5753" target="xrefwindow" rel="namesforlife-name">Eggerthella</a> (strains HF-1101T and HF-4214), closest to <a class="namesforlife" title="Enorma massiliensis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.24644" target="xrefwindow" rel="namesforlife-name">Enorma massiliensis</a> (both 98.6 %) and <a class="namesforlife" title="Eggerthella sinensis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.10314" target="xrefwindow" rel="namesforlife-name">Eggerthella sinensis</a> (98.0 and 97.8 %), respectively. The whole genome sequences of strains HF-1365T and HF-1101T were 2.3 and 4.2&thinsp;Mb in size with 61.7 and 66.2&thinsp;mol% DNA G+C content, respectively. The average nucleotide identity and digital DNA&ndash;DNA hybridization values indicated that strains HF-1365T and HF-1101T represent novel species in the genera <a class="namesforlife" title="Enorma - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.24643" target="xrefwindow" rel="namesforlife-name">Enorma</a> and <a class="namesforlife" title="Eggerthella - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5753" target="xrefwindow" rel="namesforlife-name">Eggerthella</a> . Major fatty acid constituents (&gt;10 %) of strains HF-1365T and HF-1362 were C12 : 0 (24.7 and 23.9 %), C14 : 0 (21.9 and 20.6 %) and summed feature 1 (C15 : 1iso H/C13 : 0 3OH; 12.8 and 10.8 %); those of strains HF-1101T and HF-4214 were C18 : 1 &thinsp;&omega;9c (32.4 and 33.1 %) and C16 : 0 (13.9 and 14.0 %). Strain HF-1365T had phospholipid, glycolipid, lipid and phosphoglycolipid without any known quinones, while strain HF-1101T had diphosphatidylglycerol as the major polar lipid and MK-7 (80.7 %) as the predominant quinone. On the basis of their phylogenetic and phenotypic characteristics, strains HF-1365T and HF-1101T represent two distinct species, respectively, in the genera <a class="namesforlife" title="Enorma - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.24643" target="xrefwindow" rel="namesforlife-name">Enorma</a> and <a class="namesforlife" title="Eggerthella - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5753" target="xrefwindow" rel="namesforlife-name">Eggerthella</a> , for which the names Enorma shizhengliae sp. nov. (type strain HF-1365T=CGMCC 1.17435T=GDMCC 1.1705T=JCM 33601T) and Eggerthella guodeyinii sp. nov. (type strain HF-1101T=CGMCC 1.17436T=GDMCC 1.1668T=JCM 33773T) are proposed.

Update: Ge2021a