Pu•biome

Notes

A novel Gram-positive, non-motile, non-flagellated, strictly anaerobic, non-spore-forming and dumbbell-shaped, coccoid- or chain-shaped bacterium, designated strain LZLJ-3T, was isolated from a mud fermentation cellar which has been used for the production of Chinese strong-flavour liquor for over 100&thinsp;years. Strain LZLJ-3T grew at 20&ndash;40&thinsp;&deg;C (optimum, 37&thinsp;&deg;C), at pH 6.0&ndash;8.0 (optimum, pH 8.0) and with NaCl concentrations up to 1 % (w/v; optimum, 0 %). Phylogenetic trees established based on 16S rRNA gene sequences showed that strain LZLJ-3T belonged to the genus <a class="namesforlife" title="Blautia - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13065" target="xrefwindow" rel="namesforlife-name">Blautia</a> of the family Lachnospiraceae, with the highest sequence similarity to <a class="namesforlife" title="Blautia stercoris - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.22921" target="xrefwindow" rel="namesforlife-name">Blautia stercoris</a> GAM6-1T (91.7 %) and <a class="namesforlife" title="Blautia faecicola - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.36842" target="xrefwindow" rel="namesforlife-name">Blautia faecicola</a> KGMB01111T (91.7 %). Comparative genome analysis showed that the orthologous average nucleotide identity (OrthoANI) and genome-to-genome distance (GGD) values between strain LZLJ-3T and <a class="namesforlife" title="B. stercoris - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.22921" target="xrefwindow" rel="namesforlife-name">B. stercoris</a> GAM6-1T were respectively 69.1 and 22.9 %; the OrthoANI and GGD values between strain LZLJ-3T and <a class="namesforlife" title="B. faecicola - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.36842" target="xrefwindow" rel="namesforlife-name">B. faecicola</a> KGMB01111T were respectively 70.86 and 36 % . The DNA G+C content of strain LZLJ-3T genome was 42.1&thinsp;mol%. The predominant celluar fatty acids (&gt;10 %) of strain LZLJ-3T were C16 : 0 FAME (27.9 %), C14 : 0 FAME (17.6 %) and C16 : 0 DMA (13.0 %). Arabinose, glucose and maltose could be utilized by strain LZLJ-3T as sole carbon sources for growth, with weak utilization of raffinose and l-fucose. API ZYM analysis gave positive reactions with &alpha;-galactosidase, &beta;-galactosidase, &alpha;-glucosidase and &beta;-glucosidase. The major end product of glucose fermentation was acetic acid. Based on the results of phenotypic, genotypic and phylogenetic analyses, strain LZLJ-3T is considered to represent a novel species of <a class="namesforlife" title="Blautia - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.13065" target="xrefwindow" rel="namesforlife-name">Blautia</a> , for which the name Blautia liquoris sp. nov. is proposed. The type strain is LZLJ-3T (=KCTC 25163T=CGMCC 1.5299T=JCM 34225T).

Update: Lu2021