Pu•biome

Notes

A novel Gram-stain-positive, strictly anaerobic, elliptical, non-motile and non-flagellated bacterium, designed LZLJ-2T, was isolated from the mud in a fermentation cellar used for the production of Chinese Luzhou-flavour Baijiu. Growth occurred at 28&ndash;45&thinsp;&deg;C (optimum, 37&thinsp;&deg;C), at pH 6.0&ndash;7.0 (optimum, pH 6.0) and with concentrations of NaCl up to 2 % (w/v; optimum, 0 %). On the basis of 16S rRNA gene sequence similarity, strain LZLJ-2T belonged to the genus <a class="namesforlife" title="Thermophilibacter - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40197" target="xrefwindow" rel="namesforlife-name">Thermophilibacter</a> and was most closely related to <a class="namesforlife" title="Thermophilibacter mediterraneus - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40199" target="xrefwindow" rel="namesforlife-name">Thermophilibacter mediterraneus</a> Marseille-P3256T (similarity 96.9 %), <a class="namesforlife" title="Olsenella gallinarum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40333" target="xrefwindow" rel="namesforlife-name">Olsenella gallinarum</a> ClaCZ62T (similarity 96.6 %) and <a class="namesforlife" title="Thermophilibacter provencensis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40198" target="xrefwindow" rel="namesforlife-name">Thermophilibacter provencensis</a> Marseille-P2912T (similarity 96.4 %). In addition, strain LZLJ-2T had high similarity to the genus <a class="namesforlife" title="Olsenella - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5755" target="xrefwindow" rel="namesforlife-name">Olsenella</a> , including <a class="namesforlife" title="Olsenella profusa - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5757" target="xrefwindow" rel="namesforlife-name">Olsenella profusa</a> DSM 13989T (similarity 94.9 %), <a class="namesforlife" title="Olsenella umbonata - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.21284" target="xrefwindow" rel="namesforlife-name">Olsenella umbonata</a> DSM 22620T (similarity 94.9 %), <a class="namesforlife" title="Olsenella uli - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5756" target="xrefwindow" rel="namesforlife-name">Olsenella uli</a> ATCC 49627T (similarity 94.22 %), <a class="namesforlife" title="Tractidigestivibacter scatoligenes - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40203" target="xrefwindow" rel="namesforlife-name">Tractidigestivibacter scatoligenes</a> DSM 28304T (similarity 93.9 %) and <a class="namesforlife" title="Paratractidigestivibacter faecalis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40205" target="xrefwindow" rel="namesforlife-name">Paratractidigestivibacter faecalis</a> KCTC 15699T (similarity 93.25 %). Comparative genome analysis showed that orthoANI values between strain LZLJ-2T and <a class="namesforlife" title="Thermophilibacter mediterraneus - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40199" target="xrefwindow" rel="namesforlife-name">Thermophilibacter mediterraneus</a> Marseille-P3256T, <a class="namesforlife" title="Olsenella gallinarum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40333" target="xrefwindow" rel="namesforlife-name">Olsenella gallinarum</a> ClaCZ62T, <a class="namesforlife" title="Thermophilibacter provencensis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40198" target="xrefwindow" rel="namesforlife-name">Thermophilibacter provencensis</a> Marseille-P2912T, <a class="namesforlife" title="Olsenella profusa - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5757" target="xrefwindow" rel="namesforlife-name">Olsenella profusa</a> DSM 13989T, <a class="namesforlife" title="Olsenella umbonata - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.21284" target="xrefwindow" rel="namesforlife-name">Olsenella umbonata</a> DSM 22620T, <a class="namesforlife" title="Olsenella uli - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.5756" target="xrefwindow" rel="namesforlife-name">Olsenella uli</a> ATCC 49627T, <a class="namesforlife" title="Tractidigestivibacter scatoligenes - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40203" target="xrefwindow" rel="namesforlife-name">Tractidigestivibacter scatoligenes</a> DSM 28304T and <a class="namesforlife" title="Paratractidigestivibacter faecalis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40205" target="xrefwindow" rel="namesforlife-name">Paratractidigestivibacter faecalis</a> KCTC 15699T were 78.68, 78.99, 78.29, 73.40, 74.00, 74.30, 75.08 and 77.23 %, and the genome-to-genome distance values were respectively 22.3, 22.5, 22.4, 19.6, 20.5, 19.7, 20.5 and 21.5 %. The genomic DNA G+C content of strain LZLJ-2T was 65.21&thinsp;mol%. The predominant cellular fatty acids (&gt;10 %) of strain LZLJ-2T were C18 : 1 cis 9 (33.7 %), C14 : 0 (22.0 %) and C18 : 1 cis 9 DMA (13.5 %). d-Glucose, sucrose, mannose, maltose, lactose (weak), salicin, glycerol (weak), cellobiose and trehalose (weak) could be used by strain LZLJ-2T as sole carbon sources. Enzyme activity results showed positive reactions with valine arylamidase, leucine arylamidase, crystine arylamidase, acid phosphatase, alkaline phosphatase, esterase (C4) (weakly positive), naphthol-AS-BI-phosphohydrolase, &alpha;-glucosidase and &beta;-glucosidase. The major end products of glucose fermentation were lactic acid and acetic acid. It produced skatole from indole acetic acid, and produced p-cresol from modified peptone&ndash;yeast extract medium with glucose. Based on the 16S rRNA gene trees as well as the genome core gene tree, it is suggested that <a class="namesforlife" title="Olsenella gallinarum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40333" target="xrefwindow" rel="namesforlife-name">Olsenella gallinarum</a> are transferred to genus <a class="namesforlife" title="Thermophilibacter - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40197" target="xrefwindow" rel="namesforlife-name">Thermophilibacter</a> as Thermophilibacter gallinarum comb. nov. Based on phenotypic, genotypic and phylogenetic data, strain LZLJ-2T is considered to represent a novel species of the genus <a class="namesforlife" title="Thermophilibacter - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.40197" target="xrefwindow" rel="namesforlife-name">Thermophilibacter</a> , for which the name Thermophilibacter immobilis sp. nov. is proposed. The type strain is LZLJ-2T (=KCTC 25162T=JCM 34224T).

Update: Lu2021a