Pu•biome

Notes

An obligately anaerobic, Gram-stain-positive and spore-forming strain, SNUG30386T was isolated from a faecal sample of a healthy Korean subject. The strain formed a round ivory-coloured colony and cells were chained rods with tapered ends, approximately 2.0&ndash;2.5&times;0.6&ndash;0.8 &mu;m in size. The taxonomic analysis indicated that strain SNUG30386T was within the family <a class="namesforlife" title="Lachnospiraceae - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4118" target="xrefwindow" rel="namesforlife-name">Lachnospiraceae</a> . According to the 16S rRNA gene sequence similarity, the closest species to strain SNUG30386T was <a class="namesforlife" title="Clostridium symbiosum - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.4028" target="xrefwindow" rel="namesforlife-name">Clostridium symbiosum</a> (95.6 %), followed by <a class="namesforlife" title="Enterocloster asparagiformis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.36004" target="xrefwindow" rel="namesforlife-name">Enterocloster asparagiformis</a> (94.8 %), <a class="namesforlife" title="Enterocloster clostridioformis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.36002" target="xrefwindow" rel="namesforlife-name">Enterocloster clostridioformis</a> (94.8 %) and <a class="namesforlife" title="Enterocloster lavalensis - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.36007" target="xrefwindow" rel="namesforlife-name">Enterocloster lavalensis</a> (94.6 %). The evolutionary tree based on 16S rRNA gene sequences demonstrated that strain SNUG30386T had split apart at a unique branch point far from other close relatives. Its DNA G+C content was 48.3 mol% calculated from the whole genome sequence. The major cellular fatty acids were C16 : 0 and C14 : 0. Compared to those of the closely related species, strain SNUG30386T showed distinct biochemical activities such as being unable to utilize most of carbon sources except d-glucose and l-arabinose. As a result, based on its unique phylogenetic clade and taxonomic characteristics, we conclude that strain SNUG30386T represents a novel species within the genus <a class="namesforlife" title="Clostridium - Click to open Names for Life widget" href="https://doi.org/10.1601/nm.3878" target="xrefwindow" rel="namesforlife-name">Clostridium</a> , for which the name Clostridium fessum sp. nov. is proposed. The type strain of the novel species is SNUG30386T (=KCTC 15633T= JCM 32258T).

Update: Seo2021